LOCUS       sequence-405720-        9952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An integrating plasmid containing both the inducible enotetSW4 
            promoter and the coding sequence of the TetR repressor under the 
            control of the constitutive CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Lyu XH, Yang YS, Pan ZQ, Ning SK, Suo F, Du LL
  TITLE     An improved tetracycline-inducible expression system for fission 
            yeast.
  JOURNAL   J Cell Sci. 2024 Nov 1;137(21):jcs263404. doi: 10.1242/jcs.263404. 
            Epub 2024 Nov 1.
  PUBMED    39318285
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-11-1"; volume: "137(21):jcs263404. doi"; pages: "
            10.1242/jcs.263404. Epub 2024 Nov 1"
COMMENT     Sequence Label: pDB5322
FEATURES             Location/Qualifiers
     source          1..9952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2707..3162)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2844..2865)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3056..3075
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3288..3310
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3302..3319
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3303..3319
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3329..3348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3329..3347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(3357..3980)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     3888..3907
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4061..4085)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4082..4285)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4111..4131)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4286..4665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    4973..4991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5088..5116)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5711..5731
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      5799..5986
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5854..5878)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6055..7630
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        6055..6398
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6399..7427
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMRLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVGASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      7433..7630
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7692..7712)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7692..7710)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7731..7747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7731..7747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7744..7766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7755..7771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7779..7785,7786..7803,7804..7809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7824..7845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7962..7979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8133..8721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8213..8232)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8892..9683,9684..9752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9515..9534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9753..9857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9925..9943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggttaa
     3361 gatccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc aaggccgaat
     3421 aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg taataatggc
     3481 ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg ctcttgatct
     3541 tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa tgcattctct
     3601 agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc atactgtttt
     3661 tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag taaagcacat
     3721 ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc taaagggcaa
     3781 aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa agcccgctta
     3841 ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc gagtttacgg
     3901 gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt aatcacttta
     3961 cttttatcta atctagacat atgaattcgg ggccgcggag gctggatcgg tcccggtgtc
     4021 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     4081 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4141 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     4201 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     4261 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     4321 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4381 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4441 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4501 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     4561 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     4621 accccgtaat tgattactat taataactag tcaataatca atgtcaacat ggcggtaatg
     4681 ttggacatga gccaatataa atgtacatat tatgatatgg atacaacgta tgcaatgggc
     4741 caagctcctc gaccccccgg gccccatctc ttgccccttc taagctcgct ggactcgcac
     4801 attggctacg gtggctcgcg tcgctcgctt ggtcgctcct tcaacgtacc ctaacgttcc
     4861 ggttaacgat tcttaaagaa gcgaccaatt tgttccaccg gcttctgtgg gctcaggggc
     4921 atgtcatgga agattgagag gatcggcgct tcctccaggt atatatagag tgtccctatc
     4981 agtgatagag acttgtttca gtaagaatca attagtattc tacagtaaac atcgcgggcc
     5041 gctcgggccc ggctagcatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     5101 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     5161 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     5221 ttccatggcc aacacttgtc actactttgg gttatggtct aatgtgcttt gctagatacc
     5281 cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     5341 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     5401 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     5461 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     5521 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     5581 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     5641 taccagacaa ccattacctg tcctatcaat ctaagctttc gaaagatccc aacgaaaaga
     5701 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     5761 aactatacaa aagatcttaa cgcgccactt ctaaataagc gaatttctta tgatttatga
     5821 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     5881 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     5941 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
     6001 ataacagggt aatatgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     6061 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     6121 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     6181 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcggacctg cgagcaggga
     6241 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     6301 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     6361 ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaaaaag cctgaactca
     6421 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     6481 ggctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     6541 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     6601 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
     6661 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     6721 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     6781 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     6841 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     6901 acgacaccgt cggtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     6961 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     7021 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     7081 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     7141 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     7201 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     7261 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     7321 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     7381 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataatca gtactgacaa
     7441 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     7501 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     7561 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     7621 cgctatactg ctgtcgattc gatactaacg ccgccatcca cctgcaggta atcacgtgcc
     7681 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     7741 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7801 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7861 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7921 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7981 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8041 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8101 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8161 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8221 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8281 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8341 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8401 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8461 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8521 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8581 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8641 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8701 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8761 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8821 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8881 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8941 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9001 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9061 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9121 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9181 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9241 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9301 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9361 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9421 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9481 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9541 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9601 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9661 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9721 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9781 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9841 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9901 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
