LOCUS       sequence-405816-       14954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuron-specific expression vector of dCas9-Set2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14954)
  AUTHORS   Xu SJ, Lombroso SI, Fischer DK, Carpenter MD, Marchione DM, Hamilton
            PJ, Lim CJ, Neve RL, Garcia BA, Wimmer ME, Pierce RC, Heller EA
  TITLE     Chromatin-mediated alternative splicing regulates cocaine-reward 
            behavior.
  JOURNAL   Neuron. 2021 Sep 15;109(18):2943-2966.e8. doi: 
            10.1016/j.neuron.2021.08.008. Epub 2021 Sep 3.
  PUBMED    34480866
REFERENCE   2  (bases 1 to 14954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.08.008"; journalName: "Neuron"; date: 
            "2021-09-15- 15"; volume: "109"; issue: "18"; pages: "2943-2966.e8"
COMMENT     Sequence Label: hSyn-GW-dCas9-SET2-IRES2-mCherry
FEATURES             Location/Qualifiers
     source          1..14954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(30..98,99..890)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(248..267)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1061..1649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1550..1569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1803..1820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1835..1975
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1901..1920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2061..2083
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    3331..3452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3586..4033
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    4139..4163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4187..4252
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4259..4279
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4289..4315,4316..4318,4319..6805,6806..6808,
                     6809..8392)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8447..8467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(10701..10725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(10741..11316,11317..11319,11320..11328)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10909..10926)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11136..11155
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11329..12039
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11474..11492)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11742..11761
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    12182..12770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12235..12255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12653..12664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12853..12974
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    13294..13428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14793..14811)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(14879..14954,1..29)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
       61 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      121 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      181 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      241 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
      301 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      361 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      421 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      481 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
      541 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
      601 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
      661 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      721 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      781 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
      841 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
      901 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
      961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1201 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     1741 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     1801 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     1861 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     1921 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     1981 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2041 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2101 gaaacgcgcg aggcagcatg aattattcca ttatgcacga ccccgccccg acgccggcac
     2161 gccgggggcc cgtggccgcg gcccgttggt cgaacccccg gccccgccca tccgcgccat
     2221 ctgccatggg cggggcgcga gggcgggtgg gtccgcgccc cgccccgcat ggcatctcat
     2281 taccgcccga tccggcggtt tccgcttccg ttccgcatgc taacgaggaa caggcagggg
     2341 gcggggcccg ggccccgact tcccggttcg gcggtaatga gatacgagcc ccgcgcgccc
     2401 gttggccgtc cccgggcccc ccggtcccgc ccgccggacg ccgggaccaa cgggacggcg
     2461 ggcggcccaa gggccgcccg ccttgccgcc cccccattgg ccggcgggcg ggaccgcccc
     2521 aagggggcgg ggccgccggg taaaagaagt gagaacgcga agcgttcgca cttcgtccca
     2581 atatatatat attattaggg cgaagtgcga gcactggcgc cgtgcccgac tccgcgccgg
     2641 ccccgggggc gggcccgggc ggcggggggc gggtctctcc ggcgcacata aaggcccggc
     2701 gcgaccgacg cccgcagacg gcgccggcca cgaacgacgg gagcggctgc ggagcacgcg
     2761 gaccgggagc gggagtcgca gagggccgtc ggagcggacg gcgtcggcat cgcgacgccc
     2821 cggctcggga tcgggatcgc atcggaaagg gacacgcgga cgcggggggg aaagacccgc
     2881 ccaccccacc cacgaaacac aggggacgca ccccgggggc ctccgacgac agaaacccac
     2941 cggtccgcct tttttgcacg ggtaagcacc ttgggtgggc ggaggagggg gggacgcggg
     3001 ggcggaggag gggggacgcg ggggcggagg aggggggacg cgggggcggn aggagggggg
     3061 nacgcggggg cggaggnagg ggggnacgcg ggggcggnag gagggggctc acccgcgttc
     3121 gtgccttccc gcaggaggaa cgtcctcgtc gataagcttg catgcctgca ggtcgactct
     3181 agatgatcag atcggtaccg gatccgaatt cgatatcctc gagcaacgcc gatggcagcc
     3241 tggcgggggg taccggatcc gaattcgata tcctcgagct aggctaggcc ttctagagtc
     3301 gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     3361 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     3421 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     3481 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     3541 gtaaaatcga tttaattaaa gtatctgcag agggccctgc gtatgagtgc aagtgggttt
     3601 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
     3661 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
     3721 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
     3781 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
     3841 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
     3901 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
     3961 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
     4021 cctgagagcg cagctgtgct cctgggcacc gcgcagtccg cccccgcggc tcctggccag
     4081 accaccccta ggaccccctg ccccaagtcg cagcctctag aagatctgtt taaacatcac
     4141 aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatggatt ataaagacca
     4201 cgatggcgac tacaaggacc acgacatcga ttataaggac gacgacgaca agatggcccc
     4261 caagaagaag agaaaggtcg gaagaggcat ggacaagaaa tactccattg gcctcgctat
     4321 cggcacaaac tccgtgggat gggccgtgat taccgatgaa tacaaggtgc cctccaagaa
     4381 attcaaagtg ctcggcaaca cagataggca ctccatcaag aagaacctga tcggagccct
     4441 gctctttgat tccggcgaaa ccgccgaggc taccaggctg aagagaacag ccagaaggag
     4501 atacacaagg agaaagaata ggatttgcta tctccaggag atcttcagca acgagatggc
     4561 caaggtggat gatagcttct ttcataggct ggaggaaagc ttcctggtgg aagaggacaa
     4621 gaagcacgaa agacacccca tttttggcaa tattgtggac gaggtggctt atcacgagaa
     4681 gtatcctacc atttaccacc tcaggaagaa gctggtggat agcacagaca aggctgatct
     4741 gagactcatc tacctcgccc tcgctcatat gatcaaattc agaggccact tcctcatcga
     4801 aggcgacctc aaccccgaca actccgatgt ggataaactc tttatccagc tcgtccaaac
     4861 ctacaaccag ctcttcgagg aaaatcccat caacgctagc ggcgtggatg ccaaagctat
     4921 cctcagcgcc agactcagca aatccaggag gctggagaat ctcatcgccc aactgcctgg
     4981 agagaagaag aatggcctct tcggcaacct catcgccctc tccctcggcc tcacacctaa
     5041 tttcaagtcc aatttcgatc tcgccgaaga tgctaagctc cagctgtcca aggacaccta
     5101 cgacgacgac ctcgacaacc tgctcgccca aattggagat cagtacgccg acctgtttct
     5161 ggccgctaag aatctgtccg acgccattct gctgagcgac attctgagag tcaacacaga
     5221 gattacaaag gctcccctca gcgcttccat gattaaaagg tacgatgaac atcaccagga
     5281 tctcaccctg ctcaaggccc tcgtgaggca gcagctccct gaaaagtata aagagatctt
     5341 tttcgaccag agcaagaatg gctacgccgg atacattgac ggaggcgctt cccaggagga
     5401 gttctacaaa ttcatcaagc ccatcctgga aaagatggat ggcaccgagg aactcctggt
     5461 caaactcaac agggaagacc tcctgagaaa acagagaacc ttcgataacg gctccattcc
     5521 ccatcagatt catctgggcg agctgcacgc tatcctcagg aggcaagagg acttctaccc
     5581 cttcctgaaa gacaataggg aaaagatcga aaaaatcctc acattcagga ttccttatta
     5641 cgtgggacct ctggccagag gcaatagcag gtttgcctgg atgaccagaa agagcgagga
     5701 gaccattacc ccttggaact tcgaggaagt cgtggataaa ggcgcctccg cccagtcctt
     5761 cattgagaga atgaccaact tcgataaaaa cctgcctaac gagaaggtgc tccccaagca
     5821 ttccctgctg tatgagtact ttacagtgta caatgaactg acaaaagtga aatatgtgac
     5881 cgaaggcatg aggaagcccg cctttctcag cggagagcag aagaaagcca tcgtggatct
     5941 gctcttcaag acaaatagaa aagtgaccgt caagcagctg aaggaagact acttcaagaa
     6001 aatcgagtgt ttcgactccg tggaaatctc cggcgtggag gacagattta acgccagcct
     6061 gggcacctat catgacctgc tcaagatcat caaagacaaa gatttcctcg acaacgaaga
     6121 gaacgaggat attctggagg atatcgtgct caccctgaca ctctttgaag acagggagat
     6181 gatcgaggag aggctcaaaa cctacgccca tctgttcgac gacaaggtca tgaagcaact
     6241 gaagaggagg aggtatacag gatggggcag actgagcagg aagctgatta acggcatcag
     6301 ggataagcag tccggcaaga caatcctcga ttttctgaag agcgatggat tcgctaacag
     6361 aaatttcatg cagctcattc acgatgacag cctgaccttc aaggaggaca ttcagaaggc
     6421 tcaggtgtcc ggccaaggag acagcctcca cgagcatatc gctaatctcg ccggatcccc
     6481 tgccattaaa aaaggaatcc tccagaccgt gaaggtggtc gatgagctcg tgaaggtgat
     6541 gggcaggcac aaacccgaaa acatcgtgat cgagatggct agggagaatc aaaccaccca
     6601 aaaaggccag aagaacagca gggaaagaat gaagaggatc gaggagggca tcaaggagct
     6661 gggaagccag attctgaaag aacaccctgt ggagaatacc cagctgcaga atgaaaagct
     6721 gtacctctac tacctgcaga acggcaggga tatgtacgtc gaccaggagc tcgacatcaa
     6781 taggctgtcc gactatgacg tcgacgccat tgtcccccaa agcttcctca aggatgacag
     6841 catcgataac aaggtcctca caaggtccga taaaaacaga ggaaagtccg ataatgtgcc
     6901 ctccgaagaa gtggtgaaaa aaatgaaaaa ctactggaga cagctgctga acgccaagct
     6961 gattacacag agaaaattcg acaatctgac caaggccgaa agaggcggcc tctccgaact
     7021 ggacaaggcc ggatttatca agaggcagct cgtcgaaacc aggcagatca caaaacacgt
     7081 ggctcagatc ctcgattcca ggatgaatac caaatatgac gaaaatgata aactcatcag
     7141 ggaagtgaag gtgatcaccc tcaaaagcaa actggtcagc gacttcagga aggatttcca
     7201 gttctacaaa gtgagagaaa tcaacaacta ccatcacgcc catgatgcct acctcaacgc
     7261 cgtcgtcggc accgccctca tcaagaagta tcccaaactg gagtccgagt ttgtctacgg
     7321 agattataag gtgtacgacg tgaggaagat gatcgctaag agcgaacagg aaatcggcaa
     7381 ggccacagcc aaatactttt tttacagcaa tattatgaac ttctttaaga ccgagatcac
     7441 cctggccaat ggagaaatta ggaagaggcc cctgatcgag acaaacggcg agaccggaga
     7501 gatcgtctgg gataagggca gagattttgc cacagtcagg aaggtcctct ccatgcctca
     7561 ggtcaacatc gtcaaaaaga ccgaagtcca gacaggcggc tttagcaagg agtccatcct
     7621 gcctaagagg aacagcgaca aactcatcgc tagaaaaaag gactgggatc ccaagaaata
     7681 cggcggattt gacagcccta cagtcgctta ttccgtgctg gtcgtcgcta aagtggagaa
     7741 gggaaagtcc aaaaagctca agagcgtcaa ggagctgctg ggcatcacca tcatggaaag
     7801 atccagcttc gagaaaaacc ctatcgactt cctcgaagcc aagggctaca aagaagtgaa
     7861 gaaggatctg attatcaagc tgcccaagta ttccctcttt gagctcgaga atggaaggaa
     7921 gaggatgctc gccagcgctg gagaactgca aaagggcaac gagctggccc tgccttccaa
     7981 atacgtgaac tttctgtacc tcgcctccca ctatgagaaa ctcaagggaa gccccgagga
     8041 taacgaacag aagcagctct tcgtggaaca gcacaagcac tatctcgacg agattattga
     8101 gcaaattagc gagttcagca agagggtcat cctggctgat gctaacctcg acaaagtgct
     8161 gagcgcctac aataagcaca gggataagcc cattagggag caggccgaga atatcatcca
     8221 tctgttcacc ctcacaaatc tgggcgctcc cgccgctttc aagtacttcg acaccacaat
     8281 tgataggaag agatacacca gcacaaagga ggtgctggat gctaccctca tccaccagag
     8341 cattacaggc ctgtacgaaa ccagaattga cctgtcccaa ctcggcggcg attccgctgg
     8401 cggcggaggc tccggcggcg gcggctccgg aggcggaggc tccggaccca agaaaaagag
     8461 gaaggtcgct gccgctggca gcatgtccaa gaaccagagc gtgagcgcca gcgaggatga
     8521 gaaggaaatc ctcaacaaca acgccgaggg ccataaacct cagagactgt tcgatcaaga
     8581 gcccgacctg accgaggaag ccctgaccaa attcgaaaac ctggacgact gcatctacgc
     8641 caacaagaga atcggcacat tcaagaataa cgattttatg gaatgcgact gctatgaaga
     8701 gttctccgac ggcgtgaacc acgcctgtga cgaagactcc gactgcatca acagactcac
     8761 cctgattgag tgtgtgaatg acctgtgcag ctcctgcggc aacgactgcc agaaccaaag
     8821 gtttcagaag aagcagtacg cccccatcgc catcttcaag accaaacaca agggctacgg
     8881 agtgagggct gaacaggaca tcgaggccaa ccaattcatt tacgagtaca aaggcgaggt
     8941 gatcgaggag atggagttta gggataggct cattgactac gatcagaggc actttaaaca
     9001 cttctatttt atgatgctcc agaacggaga gttcattgat gctacaatca aaggaagcct
     9061 ggctaggttt tgtaaccaca gctgttcccc taacgcctac gtcaacaagt gggtggtcaa
     9121 ggacaagctg agaatgggca tcttcgctca gaggaaaatc ctgaaaggcg aggagatcac
     9181 attcgactac aacgtggata gatacggcgc ccaagcccag aagtgctact gcgaggaacc
     9241 caattgtatt ggcttcctgg gaggaaagac ccagaccgac gccgccagcc tcctccctca
     9301 gaatatcgct gatgccctgg gagtgaccgt gtccatggag aagaagtggc tcaagctgaa
     9361 gaagctgtcc ggagagccca tcatcaaaaa tgaaaacgaa aacattaaca tcgaattcct
     9421 gcagagcctg gaggtgcagc ccatcgactc ccccgtggat gtgacaaaga tcatgagcgt
     9481 gctgctccag caggacaaca agatcatcgc cagcaagctg ctgaagaggc tcttcacaat
     9541 cgatgacgat tccctgaggc atcaggccat caaactccat ggatacacct gttttagcaa
     9601 aatgctcaag ctgtttatca cagagcagcc ccaggtcgat ggcaaaggca atgagaccga
     9661 ggaagacgat atcaagttca tcaagggcat tctcgacttc ctgctcgaac tgcccaaaac
     9721 caccagaaat ggcatcgaga gcagccagat cgataatgtc gtgaagaccc tgcctgccaa
     9781 gttccccttt ctgaagccca actgcgacga gctgctggag aaatggtcca agtttgaaac
     9841 ctacaagagg atcacaaaga aggacatcaa cgtggccgcc tccaagatga tcgacctcag
     9901 gagggtgagg ctccctcctg gctgggagat catccacgag aacggcaggc ccctctacta
     9961 caatgccgag cagaagacca aactgcacta tcctccctcc ggcagctcca aggtgttcag
    10021 ctccagatcc aacacccagg tcaactcccc tagctcctcc ggaatcccca agacacccgg
    10081 cgctctcgac tccaagaagc ataagctcag cgatgaggaa tacgagagga agaagcagaa
    10141 gaggctcgag tacgagagga tcgccctgga aagggctaag caggaagagc tggagagcct
    10201 gaagcagaag ctgaaactcg agaacgagag aaagagcgtg ctggaggata tcattgccga
    10261 agccaataag cagaaggagc tgcagaagga ggaagctaag aagctggtgg aagccaagga
    10321 agccaagaga ctcaagagga agaccgtcag ccagagccag agactcgagc acaactggaa
    10381 taaatttttc gctagcttcg tccccaacct gatcaaaaag aaccctcagt ccaagcagtt
    10441 tgaccacgaa aatatcaagc agtgtgccaa ggacatcgtg aagatcctca caaccaaaga
    10501 gctgaaaaag gatagcagca gagcccctcc tgatgacctg accaaaggaa agaggcataa
    10561 ggtgaaggag tttatcaatt cctatatgga caaaatcatt ctcaaaaaga agcaaaaaaa
    10621 ggccctcgct ctcagcagcg ccagcaccag aatgagcagc ccccccccta gcacctcctc
    10681 ctgaaagggt gggcgcgccg acccagcttt cttgtacaaa gtggtttaaa ctggtacgcg
    10741 cccctctccc gtcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
    10801 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
    10861 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
    10921 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
    10981 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
    11041 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
    11101 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
    11161 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
    11221 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
    11281 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag
    11341 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
    11401 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
    11461 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
    11521 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
    11581 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
    11641 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
    11701 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
    11761 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
    11821 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
    11881 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
    11941 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
    12001 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
    12061 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    12121 cctgaacctg aaacataaaa tgaatgcaat tgtttcccgt tatttgcact ctgttcctgt
    12181 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
    12241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
    12301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
    12361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
    12421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
    12481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
    12541 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
    12601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
    12661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
    12721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgtttcgcc
    12781 tcggcgtccg gtccgtgttg cttggtcttc acctgtgcag acttgcgaac catggattca
    12841 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12901 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12961 atcaatgtat cttaagacta gtctgagcgc cggtcgctac cattaccagt tggtctggtg
    13021 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
    13081 tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt
    13141 ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat
    13201 cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca
    13261 accgctgttt ggtctgcttt ctgacaaact cggaacttgt ttattgcagc ttataatggt
    13321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    13381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctc tgacctgaga
    13441 ttggcggcac tgaggtagag atgcccgaac ccccccgagg gagcgcggga cgcggccggg
    13501 gagggctggg gccggggagg gctggggccg gggagggctg gggccgggga gggctggggc
    13561 cggggagggc tggggccggg ggagggctgg ggctggggag gggcggtggt gtgtagcagg
    13621 agcggtgtgt tgcgccgggg tacgtctgga ggagcgggag gtgcgcggtg acgtgtggat
    13681 gaggaacagg agttgttgcg cggtgagttg tcgctgtgag ttgtgttgtt gggcaggtgt
    13741 ggtggatgac gtgacgtgtg acgtgcggag tgcgccgtgc tctgttggtt tcacctgtgg
    13801 cagcccgggc cccccgcggg cgcgcgcgcg cgcrraaarg gckggsgscg gksmgggctg
    13861 gggysgggga gggctggggc cggggrgggc tggggccggg gagggctggg gctggggagg
    13921 ggcggtggtg tgtagcagga gcggtgtgtt gcgccggggt acgtctggag gagcgggagg
    13981 tgcgcggtga cgtgtggatg aggaacagga gttgttgcgc ggtgagttgt cgctgtgagt
    14041 tgtgttgttg ggcaggtgtg gtggatgacg tgacgtgtga cgtgcggagt gcgccgtgct
    14101 ctgttggttt cacctgtggc agcccgggcc ccccgcgggc gcgcgcgcgc gcaaaaaagg
    14161 cgggcggcgg tccgggcggc gtgcgcgcgc gcggcgggcg tggggggcgg ggccgcggga
    14221 gcggggggrg gangcggggg ggggaggcgg gggggggrgc gggggggggg gnsggggggk
    14281 ggnngcggng gggggggggc gggggggggg gnggggggng ggggggcggn gggngggggg
    14341 gsgggggggg sgggsggggg gggggggggn gggnggggng gnnggggggg gggggggkgg
    14401 gggggggggg gggggggggg nggggggggg gggrggggsg ggggggaggg ngngggggag
    14461 gnagcggggg gaggagcggc cagaccccga aaacgggccc cccccaaaac acaccccccg
    14521 ggggtcgcgc gcggcccttt aaagcggcgg cggcgggcag cccgggcccc ccgcggccga
    14581 gactagcgag ttagacaggc aagcactact cgcctctgca cgcacatgct tgcctgtcaa
    14641 actctaccac cccggcacgc tctctgtctc catggcccgc cgccgccgcc atcgcggccc
    14701 ccgccgcccc cggccgcccg ggcccacggg cgccgtccca accgcacagt cccaggtaac
    14761 gactctagga attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
    14821 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
    14881 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    14941 ataaccctga taaa
//
