LOCUS       sequence-406174-       13953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Talin-1 protein  in MAC-Tag-C backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Turkki P, Chowdhury I, Ohman T, Azizi L, Varjosalo M, Hytonen VP
  TITLE     Tensin-2 interactomics reveals interaction with GAPDH and a 
            phosphorylation-mediated regulatory role in glycolysis.
  JOURNAL   Sci Rep. 2024 Aug 27;14(1):19862. doi: 10.1038/s41598-024-65787-7.
  PUBMED    39191795
REFERENCE   2  (bases 1 to 13953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-08-27"; volume: "14(1):19862. doi"; pages: " 
            10.1038/s41598-024-65787-7"
COMMENT     Sequence Label: Talin-1 WT
FEATURES             Location/Qualifiers
     source          1..13953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    252..373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(263..282)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     317..336
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(503..1525)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(503..1525)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(1533..1580)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1712..1731
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1864..2088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2077..2094
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2138..2746,2747..2749,2750..3097))
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(3098..3124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(3098..3124)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3155..3178)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3215..3238)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    3285..3309
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10939..10963)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11106..11130)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    11140..11158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11161..11179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(11181..11384)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11210..11230)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11385..11764
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     11936..11955
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        12030..12134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12135..12203,12204..12995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12353..12372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13166..13754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13655..13674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13908..13925
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta
      241 tacagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      301 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      361 caataaacaa gttggggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca
      421 tccagccggc gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg
      481 aatcgaaatc tcgtagtacg tgctattcct ttgccctcgg acgagtgctg gggcgtcggt
      541 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
      601 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
      661 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
      721 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
      781 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
      841 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
      901 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
      961 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     1021 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     1081 aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     1141 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     1201 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     1261 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     1321 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     1381 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     1441 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     1501 acgtcgcggt gagttcaggc tttttggcca aggaagttcc tatactttct agagaatagg
     1561 aacttcggaa taggaacttc taggtacgtg aaccatcacc ctaatcaagt tttttggggt
     1621 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1681 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1741 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1801 cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag
     1861 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
     1921 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
     1981 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
     2041 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
     2101 agcgggttta aacgggccct ctagactcga gtcatcactt ctcggcgctc cgcaggctga
     2161 tctcgccgcc catccagggc ttgatgatgc cgtcctgttc caggagcagg gccccctgct
     2221 tgtcgatgcc ccggctgatg ccgaagatct ctttgtcgcc gatgatcagt ttcacgggcc
     2281 ggttgatgaa gttgtccagc ttctcccatc tgctcaggta gggggccagg ccttcctgct
     2341 cgaacagttc cagggcggct ctcagctctc tgatcagcat ggcggccagg gtgttccggt
     2401 ccaggttgat gccggcttcc tgcagggtga tccagccctg gttgaccacg ctctcttcca
     2461 cccgccgcat ggccatgttg atgccagcgc cgatcacgat ctgggcggcg tcgcctgttt
     2521 tgccggtcag ttccaccagg atgccggcca gcttccggtc ctgcagatac aggtcgttgg
     2581 gccacttcac gcgcactttg tcggcgccca gcttccgcag cacctcggcc atcacgatgc
     2641 cgatgaccag gctcaggcca atagcggcgg ctgggccctg ttccagtctc cagaacatgc
     2701 tcaggtacag gttggcgccg aaggggctga accactttct gcccctgccg cctctgccgg
     2761 cctgctggta ctcggcgata caggcgtcgc cggacttcag ctcgccgatc cggtcgagca
     2821 gatactggtt ggtgctgtcg atcacgggca gcacggccac agatccgcca tccagctggc
     2881 ccaggatctg cttggcgttc agcagctgga tgggctcggg caggctgtag cccttgccgg
     2941 gcactgtgaa cacgtccacg ccccagtccc gcagggtctg gatgtgcttg ttgatggcgg
     3001 ctctgctcat gcccagtgtc tcgcccagct gctcgccaga gtggaactcg ccgttggcca
     3061 gcagggcgat cagcttcagg ggcacggtgt tgtccttggc gtagtcgggc acgtcgtagg
     3121 ggtagctggc catggtgctg cccagctcgg tgcctttctc aaactgaggg tgggaccagc
     3181 ttcctccgcc gcttccgccg ccagatcctc cgcccttctc gaactgggga tggctccacc
     3241 ccactttctt gtacaggctg gtgatgtcgg cggccttgat atcaaccact ttgtacaaga
     3301 aagctgggtc gtgctcgtct cgaagctctg aaggcaagaa cttgtactgc tgctgccgga
     3361 tctgggcgag ctttttccga gcctcttcca gctctcgttc cttccgaagc atctcttcct
     3421 gtgctgcgat aatctgggca atgcccccaa ccattttctc cttcaccacc accgtctcat
     3481 tctcctggtc ttcgaaggct gcagctttct gggccgcctt taccaggtta tctgaggccc
     3541 tcttcactgc attgccagca gcctggagcc gtttcattgc ctcagagtcc tgatctgctt
     3601 tgaccttgca agctaccagg agctgagctg tggaggcagc tacttgcttg gctgaggaaa
     3661 tgagtttctc ctggctagca tggccctgga cagctgcgtt agctgcttca cacagattgt
     3721 tggtggccgc agccaccata cgggcagcgg aaatgaggcc ctgcgaccac tgcccatcat
     3781 ccagtgcatt ggctggaatg gcgcccacct ttccttgagc caccagctcc ctctgggctg
     3841 ctgacgcagc ctttaccagt gcactggtgg ctgcagcgat ggacttggca gcttctagga
     3901 tttgttcctc aaagttcaag gactcatccg cctccttggg tttggccctg ggcttcagct
     3961 gctccagctt cttggctgca gcctcgatgg cggctgcggc tcccaagagc tcattctcag
     4021 caatgacagt agggtcctct gggtccaccc actctgttcc tttcatggct tcagcagcct
     4081 ggatgagctc agtcacagag ccagcaaccc gctttgagtg tccagttagc tgctgcttca
     4141 ggtctgggtt tggcttctga agggtcagca gcacgtggtc cagcagttcc aggtaaccat
     4201 tggcacactc ccggccatag tgcagggctc ggagccgtac atcaggcgcc acttctgggt
     4261 ggaaagctgc ttccttgcaa gcccgaagca tgtccgcaat agcccgtcgg ctcagattgg
     4321 ctgtggcaat gacatcttcc tgtcgacagg aattgccagc agcaacggct ttggctgttg
     4381 ccatagtaat acccttggtc attcggatga agtcttcagg ggtagaggtc ttggcaggtg
     4441 gctctgggga acagaagacc gccagttcct gacgtatgtg ctctgtggtt gcctctaggg
     4501 cccgtgtgcc tttggtggcc tcatcttcca cagccttcac tgtcttgagc aatgatgtca
     4561 cattggtcac catcaccttg gcagagttct tgagctgcca cactgcaggg tcatccccaa
     4621 ctttgcccgc tgcagccttc gtagcgctga tgaggtcacc cagggccttg gctacgtcct
     4681 ttactgcatt gatcagcacc acctgagttt cagggtcttc ggctcctagg ctggctgcac
     4741 cgagcttgac cacatcagcg aggcgggtaa tggtggccac ggaggactgg gcggcttgtg
     4801 ccaacttctc ctggctccca gctgcattct gcactaggac cttggtgtcc tccacaagaa
     4861 cctttgctgt ctttaagata ccctcccggt ggtcagcaaa agtttcagca ccctcacggt
     4921 taagtgtgcc agcagtagcg aacatgatgg tggtgtcgag gtcagcaatg ataccagaca
     4981 cagcactggc tgctgtaatg caggcctggg taccacgatt cccagcctgg agtgcagcca
     5041 gaacatggga gaccttctct gacactctgc gggcacactc tatgagctcc ttcttggtgt
     5101 agacatcact ggggctacac tgcaaggcac ctgccttggt gaccagagca gagcagccat
     5161 ggcccagctc ctgtactcgg tgtttgatgt gagcgcctat ctcttcattt tcagcagcca
     5221 cagctgcagg cttggcttgt gaggccagtc ggccatagtc actggtcagc tggttggcaa
     5281 gagggcctag ctcttcaggg ctggtgtttg acttggttac catctcctga acagtgacag
     5341 caatggcctt ggctgtcctc accatggttg tctggtaatc tacgaatgag ccttctgggt
     5401 cacccatagg tccttcatct agctggttga tggcctgagt gatagagtcc accatgccgc
     5461 caacgactcc tgcagcactg gctgcttcgt tgagggtcgt cgtcaggtct tctacggcct
     5521 ctgtcatcat ctgcacagcc tcctccaggg cttcctgggt gtgtgctgct tgcttggggt
     5581 taccaccagc ctccttggca gtatatagca actgcaaggc agactctgcc aacgttttag
     5641 tctggtccag aagtgccatc tgctgcgggt ggctcagggt cttagacgca gcacccactg
     5701 cagccagggt gagtggttca aagtactggg ccatttggga caccttgtgt cccagctggg
     5761 aggcttcagc ccgagcagcg ctggccagcg gctcaatgag atgagaaatt tcttgcactg
     5821 cagtgagcat ctgggtgtgc aaagcctctt gagagattcc ttcacgggga gcaagctgct
     5881 ggctgacagc ggcaagcgaa gcctgatcta ggtcccgcaa gcagctgttc agagccgcaa
     5941 tggctgtctc acactccagc tgccctgggg ctttgtccct catgcttgta ataagtttct
     6001 tgatggaatc tgagacagtc cgagagtggc cagctagcac agaccaacgt ggggggtctc
     6061 ggggattgac tgctaacgcc cgagctgtct ggatgagccc tccagcactc tccaacattg
     6121 tcttagcaga gattacaatg ggctccatgg ctgctctgcc ctcagggctg atctgggcag
     6181 ggacgctgga gaactcaggg ttggaggcaa aggcactcag gttatccaca gcttccagca
     6241 gaggggctgt ggctgctcgg cactgggcac ggttctcttc tgtgaagtcc ccatctagtg
     6301 ccttgatggt cttgacaaga ttggctgtac tgttggccac ctccttggct gactgtacaa
     6361 actggcgctt ggcagtggga ttggcagtcc tagcggaagc caggcgacag ctgttacaca
     6421 atgcagatgt gtgtttggct acaatagtgg ctgcagataa cacctgggcc tgggtacagc
     6481 caggctcccc cagactctga caggccatct gaattgcctg gtttgcacgg gcaaactgtg
     6541 tgggttccac cagtccttgc tgtccagctt ggctgttggg gtcagagaca ccaactagat
     6601 atgctgcctg tgcggctgcc tccgtgaagc cacagagagc cttggaggct gtggcaatgg
     6661 catctccaaa ctccggcaga tttccattct tggcattttg ggagatgcca gtcatggcct
     6721 cacctaggac cttggagttc tccatgacac tgtccaagca accgaagtag gacatgtcgt
     6781 tgatgggctg gactggattc tccaggagtt ctcggactgt ctctagctgc cgaagtgcat
     6841 tgtcacactc cttctggcca ggtgcctgct gggtgcacat ggtgatgagc tggttgatgc
     6901 tgtccgtcac tgcccgggca gccgcagcca gctgactctt gaggttggga gaagcagggt
     6961 ctgtggacaa ggccttagcg gcaaggagaa gtttgcttga agacatggat atgcccttca
     7021 ggttggacac cacctgggct cggtcctcct ggctcggggc ctgtccggcc atctccacac
     7081 cagcttccag gaaggtgctg aagtcctgtc caaatcggcc ggaggcccga gccaggtcct
     7141 gaggagttcc tcgggaggct tgcaccagtt ctgtggcggc ctgatttaac ccagctgcag
     7201 cttcattcaa tcggctctgt gcttcttgaa atgtccccgt gcttggagga agcaagtcac
     7261 tcaggagtcg cttgctggca tctccaacgg ctcgtagggc attatccacg tctctttggc
     7321 caggcaggca gctgacacag cggttcaggg cttgggtcac tgctttagct acctgagcaa
     7381 gcctttgctg gctttctggg tcccctgggt gtccagatgc tttttttgcc tcttcaatga
     7441 ggctgctggc cttgtccaga acgtcgctgg ctgtgtcaag cacaatggcc tgcactgcag
     7501 gatctgatgt cagcgcagcc acaccccgcg cagcctgggc tagtgacctt agtccaccag
     7561 ctacatcccg agctgcaata cctgcatagt tctcgttgcc ctgggcaatc tcccccagca
     7621 gcttggcgat ggcagagctc accgctttgg tgctgttgcc aagatcttgg gtacacttct
     7681 ccattgtttc cccaggtaag ggcttaagct tgccatctcg agctgctgct tttatttcct
     7741 gtaggtcttt ctctagattt tgtacaacac tcagtgcaga atccatctcc aaaggtccac
     7801 atgcctcctg agccttctga gcagcagtgc gcagctcggc cagggcagtg cctaggttct
     7861 ttgcgcactg actcagctgc atcgctgaag cctggtcctg aattgttggc actgaggctt
     7921 ttgccgctgc caccatcttc ccacctggct gcaggaagct ctggctggca gcaatgaggg
     7981 cgagctgagc actagggctg tcaggctgag cttgacttcc tcggacaccc tgcaccagca
     8041 acggaatctg ctctgccacg gccttacagc tctgcaccag taggggctgg gggcccgcag
     8101 aggccttggg ggcagaggct gcatgttggg ctgcagcaat ggtctgtgtt gcagaggctg
     8161 cagcttgctt ggctgcatgc tccaggcgct gcaccaactt cttcttgatg gcgttctgcg
     8221 cagctgcatt ggtggccatg cgaagcccct cagctgcttc acgcagtcgc tgctgctgtt
     8281 cctcactgtc agggtgggcg gctgctccct tggccgcctc caccatcttg gcggtggcat
     8341 cagcgaggat cttggcagca ctcaggagct ttctagagtt ctccagatca ctctccccct
     8401 cagcatcggc cttgatagca ttgaccaggt ctgaggtggc ttgggccagg atgcgggcct
     8461 ggcgcaccat ctccccagca tcacccatgg agctgaagat gttttcagtg acggtgagga
     8521 tggtatcagt ggcttggtca taccgaccgg caggcccagc tccggtggcg tgggccttca
     8581 cgtgctgcag cagctcattg agggcctggg tgacagccgt ggctgctgct cctacccctc
     8641 gaagcagctg tccatcttct gtagctgctt gcgaggcaga cacacagccc tccacagctt
     8701 tggccaccag tcgcccagcc tcgactagct gttcttggca cacaggtgag ctgattgtag
     8761 gtgctaccac cttggtacag gccaccagct gggaagtgga cagagcacat tgcgtagctg
     8821 cagcaatcac ttgggtctga agcccggaat cctctgtgcg ctgggccaca ctcttggcct
     8881 tgaggaccag ggcggctgca gcacttgcca ctgcgtttgc tagctgcatt agaacatcct
     8941 ggaagtgggg gtcagtgtca ctttccccaa tttgctgcaa cagctcccca ctggcctggc
     9001 ccacgttccc ggctgcctgc agcaggttct gacgaggctc agcgctggcg ggctgagcac
     9061 tgcgaagcag ttccgacaca gcccctgcaa ggccctttgc tgcttgcaga aggggccggc
     9121 cattgccgcc ttcatcctcc agcagggcag ccaatagctt caccccacgg gacatctccg
     9181 tcaggttgga agagatggtg gtgactgcac agcccactgc tgtatagtct gtctctgcag
     9241 gatctcctgc tgtcaggttt accacagagg cagtgccagc tgtgatggca tctacctggg
     9301 agtggatctc atgctttgat tcatccatct tgttcttacg ccaagctttg gaggcagcat
     9361 cctggccaag agggggtaga gtctcaaagt catccagagt ggcctgggca gcctgcacag
     9421 cctgcatgct ggagttaatg gttccagtga gcgcctgctg ggctgaagtc agaggtggca
     9481 tgtgtcctcg gtgcatctgg ccactggtga tctgctgctg ggcaggtggc atgctcccca
     9541 cctggaaatt ctcaggacca gaggctccag agcgcatgat ggctggcaga gccacagagc
     9601 cgtgctccac tttccccact cggttgtact gctgctgaag gactgttgac tttttgggag
     9661 acactgagtc ctccaacata gtagactctt catctccctc cagcccaaaa tggtccttgc
     9721 tttttttctt cttaaggatg atatctatgt agccagcaat gagctgtgcg atctgctcgc
     9781 cttctgtcgt ctgtactgag tagtagccat cctgatagtc tccaaagtcc agagtaaagc
     9841 tcttgggaga ggcagcccag cgtttgatgt tggtgaggct ccattcctga atcacctcct
     9901 tggtcttctc atccacacgc atcacacact ccttagtgat gcccaacagc ctgggcacca
     9961 gtttattctt ccccttcatc ttttccttga ctaggaagaa ggagacaccg taagtcttga
    10021 gggaacgggc cagcttcacg tagcgtacct tggcctcaat ttcactcatc tgcccacaat
    10081 tcttgtgtgc ctgaaagatc ttacgctctc ccttctgctt cacatactcc ttgggcagga
    10141 agtccttcag gtcaaggaag ccagccttgt gcttctgttc attgtgaggt ccaaactgga
    10201 tctggcactg gaagcctgca aattcacagg ccttgtcaaa ggagacagga tgggagccat
    10261 tcaggatgtc atctcgtgcc tgcacataga gaaggttcag ctgtacaggg tctcgggaat
    10321 ccacattctg gtctgagtag aagaactttc tccgcagcag caacgtctca tgctcttcca
    10381 ctccctgttc cctcagtgtc cgcccatggt ccagccagtt caactcatcg tctgtgtgca
    10441 acttctgctt tagcttctcc atcttctttt catctcgcag tagggtcttg tcttttctca
    10501 gggtccctgt cccctcatct ttcttttctt ccatcagctc tcgaaccagt gaatactcat
    10561 catggttggt gataccaatt cgggcacaaa tggtcatgag catgtctgtg acggtcttgg
    10621 agtcatccac catgatagtc ttcaccgttc cgtctaacat ccggatcttc aggggtctct
    10681 gcttttttct gtactccatg gtgtccccat ttcggagcat gtagtagtcc aaagctttcc
    10741 cagcctctag ccagatgcct tttttggggt catcatctga cagaaagagc ccaaagtcgt
    10801 tgggagggcc agccagggcc tctgggatcc gctcacgaat catgcggcag gcgtcgtaca
    10861 ccatggtaga tggctcaaat tgcatcgtct tcaccacatt cccaatgcta atcttcagcg
    10921 aaagcgcaac catggtggag cctgcttttt tgtacaaact tgttgatatc tgcagaattc
    10981 caccacactg gactagtgga tccgagctcg gtaccaagct taagtttaaa cgctagagtc
    11041 cggaggctgg atcggtcccg gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc
    11101 caggcgatct gacggttcac taaacgagct cgtcgacgat ctctatcact gatagggaga
    11161 tctctatcac tgatagggag agctctgctt atatagacct cccaccgtac acgcctaccg
    11221 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
    11281 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
    11341 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
    11401 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
    11461 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
    11521 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
    11581 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
    11641 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
    11701 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
    11761 tgtcaacgcg tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc
    11821 agattcttca tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg
    11881 cactactcag cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca
    11941 tcagagcaga ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga
    12001 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    12061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    12121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    12181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    12241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    12301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    12361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    12421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    12481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    12541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    12601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    12661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    12721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    12781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    12841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    12901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    12961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    13021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    13081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    13141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    13201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    13261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    13321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    13381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    13441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    13501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    13561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    13621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    13681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    13741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    13801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    13861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    13921 gcgaggaagc ggaagagcgc ccaatacgca aac
//
