LOCUS       sequence-406249-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
            C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, 
            Liu DR
  TITLE     Enhanced prime editing systems by manipulating cellular determinants
            of editing outcomes.
  JOURNAL   Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 
            10.1016/j.cell.2021.09.018.
  PUBMED    34653350
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.09"; journalName: "Cell"; date: "2021-10-28- 
            28"; volume: "184"; issue: "22"; pages: "5635-5652.e29"
COMMENT     Sequence Label: pCMV-PEmax
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1648..1668)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1717..1736)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1718..1736)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1778..1981)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1807..1827)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1982..2361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2533..2552
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2631..2735
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2736..2804,2805..3596)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2954..2973)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3767..4355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4256..4275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4509..4526
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4643..4664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4679..4684,4685..4702,4703..4709)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4717..4733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4722..4744
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4741..4757
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4741..4757
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    4828..5052
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5041..5058
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(5081..5098)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(5107..5133)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(5143..5163)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7264..7284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(7342..9795,1..1647))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gtccacgatg gcctttttct gctcgccgct caggaaggcg ggctttctca ttccctcggt
       61 cacgtatttc actttggtca gctcgttata cacggtgaag tactcgtaca gcaggctgtg
      121 cttgggcagc accttctcgt tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa
      181 gctctgggcg gaagcgccct tgtccaccac ttcctcgaag ttccaggggg tgatggtttc
      241 ctcgctcttt ctggtcatcc aggcgaatct gctgtttccc ctggccagag ggcccacgta
      301 gtaggggatg cggaaggtca ggatcttctc gatcttttcc cggttgtcct tcaggaatgg
      361 gtaaaaatct tcctgccgcc gcagaatggc gtgcagctct cccaggtgga tctggtgggg
      421 gatgctgccg ttgtcgaagg tccgctgctt ccgcagcagg tcctctctct tcagcttcac
      481 gagcagttcc tcggtgccgt ccatcttttc caggatgggc ttgatgaact tgtagaactc
      541 ttcctggctg gctccgccgt caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa
      601 aatctctttg tacttctcag gcagctgctg ccgcacgaga gctttcagca gggtcaggtc
      661 ctggtggtgc tcgtcgtatc tcttgatcat agaggcgctc aggggggcct tggtgatctc
      721 ggtgttcact ctcaggatgt cgctcagcag gatggcgtcg gacaggttct tggcggccag
      781 aaacaggtcg gcgtactggt cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta
      841 ggtgtccttg ctcagctgca gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt
      901 gggggtcagg cccaggctca gggcaatcag gtttccgaac aggccattct tcttctcgcc
      961 gggcagctgg gcgatcagat tttccagctt tctgctcttg ctcagtctgg cagacaggat
     1021 ggccttggcg tccacgccgc tggcgttgat ggggttttcc tcgaacagct ggttgtaggt
     1081 ctgcaccagc tggatgaaca gcttgtccac gtcgctgttg tcggggttca ggtcgccctc
     1141 gatcaggaag tggccccgga acttgatcat gtgggccagg gccagataga tcagccgcag
     1201 gtcggccttg tcggtgctgt ccaccagttt ctttctcagg tggtagatgg tggggtactt
     1261 ctcgtggtag gccacctcgt ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt
     1321 atcctcttcc accaggaagg actcttccag tctgtggaag aagctgtcgt ccaccttggc
     1381 catctcgttg ctgaagatct cttgcagata gcagatccgg ttcttccgtc tggtgtatct
     1441 tcttctggcg gttctcttca gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag
     1501 ggctccgatc aggttcttct tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt
     1561 cttgctgggc accttgtact cgtcggtgat cacggcccag cccacagagt tggtgccgat
     1621 gtccaggccg atgctgtact tcttgtcgac tttccgcttc ttctttggtg actcgaactc
     1681 gcttccgtcg gctgtccgtt tcatggtggc ggctctccct atagtgagtc gtattagcgg
     1741 ccgcggatct ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc
     1801 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     1861 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     1921 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     1981 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     2041 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     2101 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     2161 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     2221 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     2281 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     2341 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     2401 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     2461 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     2521 ggcttaacta tgcggcatca gagcagattg tactgagagt cgaccctagg ggatcgggag
     2581 atcgatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2641 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2701 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2761 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2821 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2881 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2941 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     3001 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3061 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3121 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3181 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3241 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3301 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3361 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3421 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3481 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3541 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3601 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3661 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3721 cgttccactg agtgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3781 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3841 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3901 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3961 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4021 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4081 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4141 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4201 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4261 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4321 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4381 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4441 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4501 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     4561 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4621 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccctaggctt
     4681 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4741 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatcgagc
     4801 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     4861 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     4921 gacacctact cagacaatgc gatgcaattt tctcatttta ttaggaaagg acagtgggag
     4981 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     5041 tagaaggcac agtcgaggct gatcagcggg tttaaactca atggtgatgg tgatgatgac
     5101 cggttagtcc agcttcactc tcttagcggc agggccagag ccgactttcc tcttcttctt
     5161 gggagactcg aattcgctgc cgtcggcggt tctttttgag ccgccagaag gggaggagtt
     5221 ttcaatcagc agagtggaag tgtcgggagt ttcagtaata gcagcctttc tggcggcctg
     5281 gtcggccatc cgattgcccc ttgcctcggc ggagtgtccc ttctggtgtc caggacagtg
     5341 gatgatgctc agccgcttgg gcagaaacag ggccttcagc agggccagga tctcatcctt
     5401 gttcttgatc tccttgccct cagaggtcag ccagccccgg cgcctgtaga tctctccgtg
     5461 gatgtgtgcg gtggcgaagg catatctgga gtctgtgtac acgttcagct tcttgccctc
     5521 ggccatcttc agggcctggg tcagggcgat cagctcggcc cgctgggcgg atgtgcctgc
     5581 tggcagggcc ttggcccaga tcacttctgt ctctgtggtc actgctgctc ctgccttcct
     5641 ctggccctcc tgcagcaggg agcttccatc ggtataccat gtgtgatcgg cgtcaggcag
     5701 gggctgatcg gtcaggtctg gccttgttcc gtgtgcctct gccaggatgt ccagacagtt
     5761 gtgctgcagg ccctcctctg gcagaggcag cagggtggct ggattcaggg ccaccacagg
     5821 gccaaactgc acgcgatcgg tgtccagcag cagggcctgg tagtgtgtca tccgggcgtt
     5881 agacagccag cgatctggag gctgcttcac cagggcctcc actgcgtgtg gtgccaggat
     5941 gaccagtggc tgtcccatgg tcagcttgcc ggcatccttt gtcagcacgg cgattgctgc
     6001 caccatcctc aggcatggtg gccatcctgc tgccactggg tccagcttct tggacagata
     6061 ggccacgggc cgtctccatg gtcccagctt ctgggtcagc acgcccttgg cgtagccctg
     6121 cttctcatcc acaaacagct cgaaaggctt ggtcaggtct ggcaggccca gggctggtgc
     6181 tgtcagcagg gcctgcttga tctcctgata ggccttctgc tggtcagggc cccaattaaa
     6241 cagggtgccc ggcttggtca gtgggtacag aggtgctgcc atctctgcga atcctgggat
     6301 gaacagcctg caaaatcctg ctttgcccag gaactccctc agctgtcttg gggtcttggg
     6361 tgttggctgg cccatcactg tctccttcct ggcctctgtc agccatctct ggccctcctt
     6421 cagcagatag cccaggtact tcacctgctt ctgacagatc tgggccttct tggcggatgc
     6481 cctatatccc aggtttccca gggtctgcag cagggcgcgt gttccctgct ggcaatccag
     6541 ctcgctggtg gcggccagca gcagatcgtc cacgtactgc agcaggatca gatctgggtg
     6601 ctggatccgg aaatctgcca ggtcgcggtg cagggcctcg ttaaacagtg ttgggctatt
     6661 cttgaagccc tgaggcaggc gtgtccaggt cagctggcca gagatgccca tctcagggtc
     6721 cctccactca aaggcgaaca gtggctggct ggtagggtgc agtctcagac aaaagaaggc
     6781 atccttcagg tccagcactg tataccactg gtgagaaggg ggcaggccgg acagcagatt
     6841 gtaagggttg ggcacggtgg ggtggatatc ctccaccctc ttgttcacct ctctcagatc
     6901 ctgcacgggt ctatagtcat tggtgcctgg cttcttcacg ggcagcagtg gtgtgttcca
     6961 ggggctctgg catggcacca ggatgccctg gtccagcagc ctctggatgt gaggcttgat
     7021 gcccagtctg gcctcctgag acatagggta ctgcttgatg ctcacgggtg tagaggtggc
     7081 cttcagaggg atgatcaggg gtgcctgccg cacggccagg cccattcctc ctgtctcggc
     7141 ccaggcctgg ggaaagtcgg acagccaggt ggagcccagg gacacgtcgg gttccttgct
     7201 tgtttcatgc agtctatact cgtcttcaat gttcagggtg cttccgccgc tagagcctcc
     7261 gctcaccttt cttttcttct tagggctctc gaattcagag ccgtcggcgg tccgcttgct
     7321 gccaccagag cttccgccgg agtcacctcc cagctgagac aggtcgatcc gtgtctcgta
     7381 caggccggtg atgctctggt ggatcagggt ggcgtccagc acctctttgg tgctggtgta
     7441 cctcttccgg tcgatggtgg tgtcaaagta cttgaaggcg gcaggggctc ccagattggt
     7501 cagggtaaac aggtggatga tattctcggc ctgctctctg atgggcttat cccggtgctt
     7561 gttgtaggcg gacagcactt tgtccagatt agcgtcggcc aggatcactc tcttggagaa
     7621 ctcgctgatc tgctcgatga tctcgtccag gtagtgcttg tgctgttcca caaacagctg
     7681 tttctgctca ttatcctcgg gggagccctt cagcttctca tagtggctgg ccaggtacag
     7741 gaagttcaca tatttggagg gcagggccag ttcgtttccc ttctgcagtt cgccggcaga
     7801 ggccagcatt ctcttccggc cgttttccag ctcgaacagg gagtacttag gcagcttgat
     7861 gatcaggtcc tttttcactt ctttgtagcc cttggcttcc agaaagtcga tgggattctt
     7921 ctcgaagctg cttctttcca tgatggtgat ccccagcagc tctttcacac tcttcagttt
     7981 cttggacttg cccttttcca ctttggccac caccagcaca gaataggcca cggtggggct
     8041 gtcgaagccg ccgtacttct tagggtccca gtccttcttt ctggcgatca gcttatcgct
     8101 gttcctcttg ggcaggatag actctttgct gaagccgcct gtctgcacct cggtcttttt
     8161 cacgatattc acttggggca tgctcagcac tttccgcacg gtggcaaaat cccggccctt
     8221 atcccacacg atctccccgg tttcgccgtt tgtctcgatc agaggccgct tccggatctc
     8281 gccgttggcc agggtaatct cggtcttgaa aaagttcatg atgttgctgt agaagaagta
     8341 cttggcggta gccttgccga tttcctgctc gctcttggcg atcatcttcc gcacgtcgta
     8401 caccttgtag tcgccgtaca cgaactcgct ttccagctta gggtactttt tgatcagggc
     8461 ggttcccacg acggcgttca ggtaggcgtc gtgggcgtgg tggtagttgt tgatctcgcg
     8521 cactttgtaa aactggaaat ccttccggaa atcggacacc agcttggact tcagggtgat
     8581 cactttcact tcccggatca gcttgtcatt ctcgtcgtac ttagtgttca tccgggagtc
     8641 caggatctgt gccacgtgct ttgtgatctg ccgggtttcc accagctgtc tcttgatgaa
     8701 gccggcctta tccagttcgc tcaggccgcc tctctcggcc ttggtcagat tgtcgaactt
     8761 tctctgggta atcagcttgg cgttcagcag ctgccgccag tagttcttca tcttcttcac
     8821 gacctcttcg gagggcacgt tgtcgctctt gccccggttc ttgtcgcttc tggtcagcac
     8881 cttgttgtcg atggagtcgt ccttcagaaa gctctgaggc acgatagcgt ccacatcgta
     8941 gtcggacagc cggttgatgt ccagttcctg gtccacgtac atatcccgcc cattctgcag
     9001 gtagtacagg tacagcttct cgttctgcag ctgggtgttt tccacggggt gttctttcag
     9061 gatctggctg cccagctctt tgatgccctc ttcgatccgc ttcattctct cgcggctgtt
     9121 cttctgtccc ttctgggtgg tctggttctc tctggccatt tcgatcacga tgttctcggg
     9181 cttgtgccgg cccatcactt tcacgagctc gtccaccacc ttcactgtct gcaggatgcc
     9241 cttcttaatg gcggggctgc cggccagatt ggcaatgtgc tcgtgcaggc tatcgccctg
     9301 gccggacacc tgggctttct ggatgtcctc tttaaaggtc aggctgtcgt cgtggatcag
     9361 ctgcatgaag tttctgttgg cgaagccgtc ggacttcagg aaatccagga ttgtcttgcc
     9421 ggactgcttg tcccggatgc cgttgatcag cttccggctc agcctgcccc agccggtgta
     9481 tctccgccgc ttcagctgct tcatcacttt gtcgtcgaac aggtgggcat aggttttcag
     9541 ccgttcctcg atcatctctc tgtcctcaaa cagtgtcagg gtcagcacga tatcttccag
     9601 aatgtcctcg ttttcctcat tgtccaggaa gtccttgtcc ttgataattt tcagcagatc
     9661 gtggtatgtg cccagggagg cgttgaaccg atcttccacg ccggagattt ccacggagtc
     9721 gaagcactcg attttcttga agtagtcctc tttcagctgc ttcacggtca ctttccggtt
     9781 ggtcttgaac agcag
//
