LOCUS       sequence-406445-        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector for transposome-mediated saturation mutagenesis and Tn-Seq in
            S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Lo H-Y, Long DR, Holmes EA, Penewit K, Hodgson T, Lewis JD, Waalkes 
            A, Salipante SJ
  TITLE     Transposon sequencing identifies genes impacting Staphylococcus 
            aureus invasion in a human macrophage model.
  JOURNAL   Infect Immun. 2023 Oct 17;91(10):e0022823. doi: 
            10.1128/iai.00228-23. Epub 2023 Sep 7.
  PUBMED    37676013
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2023-10-17"; volume: "91(10):e0022823. doi"; pages: 
            " 10.1128/iai.00228-23. Epub 2023 Sep 7"
COMMENT     Sequence Label: pAureus-TnCAM
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    10..28
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    943..964
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    999..1019
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    1093..1111
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 ggcgcccagc tgtctcttat acacatctgg gcccggatcc gtcgactcat gacctgcagg
       61 aaacactttt ttgtttactt ctcattttta attagttata attaactaaa taatagagca
      121 ttaaatatat ttaataaaac ttatttaatg caaaattatg actaacatat ctataataaa
      181 taaagattag atatcaatat attatcgggc aaatgtatcg agcaagatgc atgcatcaaa
      241 ttaggaggat gattatttat gaactttaat aaaattgatt tagacaattg gaagagaaaa
      301 gagatattta atcattattt gaaccaacaa acgactttta gtataaccac agaaattgat
      361 attagtgttt tataccgaaa cataaaacaa gaaggatata aattttaccc tgcatttatt
      421 ttcttagtga caagggtgat aaactcaaat acagctttta gaactggtta caatagcgac
      481 ggagagttag gttattggga taagttagag ccactttata caatttttga tggtgtatct
      541 aaaacattct ctggtatttg gactcctgta aagaatgact tcaaagagtt ttatgattta
      601 tacctttctg atgtagagaa atataatggt tcggggaaat tgtttcccaa aacacctata
      661 cctgaaaatg ctttttctct ttctattatt ccatggactt catttactgg gtttaactta
      721 aatatcaata ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat
      781 aaaggtaatt caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt
      841 tatcatgcag gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg
      901 cttttataat gataatcccg ggctcgaggt cgacgtttct tcgaattcgc ggccgcttct
      961 agagcgcaaa aaaccccgct tcggcggggt tttttcgcta ctagtagcgg ccgctgcagg
     1021 aagaaacagt cgacctgcag gcatgcaacg ttgccaacga ctacgcacta gccaacaaga
     1081 gcttcagggt tgagatgtgt ataagagaca gctgaagctt
//
