LOCUS       sequence-406491-        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent somatostatin (SST) 
            sensor GRAB_SST1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_SST1.0
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1035..1056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1162..1183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1423..1442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtgagc ccctgttccc agcctccacg
      121 cccagctgga acgcctcctc cccgggggct gcctctggag gcggtgacaa caggacgctg
      181 gtggggccgg cgccctcggc aggggcccgg gcggtgctgg tgcccgtgct gtacctgctg
      241 gtgtgtgcgg ccgggctggg cgggaacacg ctggtcatct acgtggtgct gcgcttcgcc
      301 aagatgaaga ccgtcaccaa catctacatt ctcaacctgg cagtggccga cgtcctgtac
      361 atgctggggc tgcctttcct ggccacgcag aacgccgcgt ccttctggcc cttcggcccc
      421 gtcctgtgcc gcctggtcat gacgctggac ggcgtcaacc agttcaccag tgtcttctgc
      481 ctgacagtca tgagcgtgga ccgctacctg gcagtggtgc acccgctgag ctcggcccgc
      541 tggcgccgcc cgcgtgtggc caagctggcg agcgccgcgg cctgggtcct gtctctgtgc
      601 atgtcgctgc cgctcctggt gttcgcggac gtgcaggagg gcggtacctg caacgccagc
      661 tggccggagc ccgtggggct gtggggcgcc gtcttcatca tctacacggc cgtgctgggc
      721 ttcttcgcgc cgctgctggt catctgcctg tgctacctgc tcatcgtggt gaaggtgagg
      781 gcggcgggcg tgcgcgtggg ctgcgcgctg gacctggagg agggaggaaa cgtctatatc
      841 aaggccgaca agcagaagaa cggcatcaag gcgaacttcc acatccgcca caacatcgag
      901 gacggcggcg tgcagctcgc ctaccactac cagcagaaca cccccatcgg cgacggcccc
      961 gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca aactttcgaa agaccccaac
     1021 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactattggc
     1081 atggacgagc tgtacaaggg cggtaccgga gggagcatgg tgagaaaggg cgaggagctg
     1141 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1201 agcgtgtccg gcgagggtga gggcgatgcc accgagggca agctgaccct gaagttcatc
     1261 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1321 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1381 atgcccgaag gctacatcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1441 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1501 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa caccggagca
     1561 gcagcacgct ggcgcgggcg gcagctcaag gtgacgtgga tggtgttggt ggtggtgctg
     1621 gtgtttgcgg gatgttggct gcccttcttc accgtcaaca tcgtcaacct ggccgtggcg
     1681 ctgccccagg agcccgcctc cgccggcctc tacttcttcg tggtcatcct ctcctacgcc
     1741 aacagctgtg ccaaccccgt cctctacggc ttcctctctg acaacttccg ccagagcttc
     1801 cagaaggttc tgtgcctccg caagggctct ggtgccaagg acgctgacgc cacggagccg
     1861 cgtccagaca ggatccggca gcagcaggag gccacgccgc ccgcgcaccg cgccgcagcc
     1921 aacgggctta tgcagaccag caagctgtaa
//
