LOCUS       sequence-406505-        2127 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent neurotensin (NTS) 
            sensor GRAB_NTS1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_NTS1.0
FEATURES             Location/Qualifiers
     source          1..2127
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1194..1215
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1321..1342)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1582..1601)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtcgcc tcaacagctc cgcgccggga
      121 accccgggca cgccggccgc cgaccccttc cagcgggcgc aggccggact ggaggaggcg
      181 ctgctggccc cgggcttcgg caacgcttcg ggcaacgcgt cggagcgcgt cctggcggca
      241 cccagcagcg agctggacgt gaacaccgac atctactcca aggtgctggt gaccgccgtg
      301 tacctggcgc tcttcgtggt gggcacggtg ggcaacacgg tgacggcgtt cacgctggcg
      361 cggaagaagt cgctgcagag cctgcagagc acggtgcatt accacctggg cagcctggcg
      421 ctgtccgacc tgctcaccct gctgctggcc atgcccgtgg agctgtacaa cttcatctgg
      481 gtgcaccacc cctgggcctt cggcgacgcc ggctgccgcg gctactactt cctgcgcgac
      541 gcctgcacct acgccacggc cctcaacgtg gccagcctga gtgtggagcg ctacctggcc
      601 atctgccacc ccttcaaggc caagaccctc atgtcccgaa gccgcaccaa gaagttcatc
      661 agcgccatct ggctcgcctc ggccctgctg gcggtgccta tgctgttcac catgggcgag
      721 cagaaccgca gcgccgacgg ccagcacgcc ggcggcctgg tgtgcacccc caccatccac
      781 actgccaccg tcaaggtcgt catacaggtc aacaccttca tgtccttcat attccccatg
      841 gtggtcatct cggtcctgaa caccatcatc gccaacaagc tgaccgtcat ggtacgccag
      901 gcggccgagc agctcaacgg cgcccctggc gagcccgcgc cggccgggcc gcgcgacacc
      961 gacgcgctgg acctggagga gggaggaaac gtctatatca aggccgacaa gcagaagaac
     1021 ggcatcaagg cggcgttcaa tatccgccac aacatcgagg acggcggcgt gcagctcgcc
     1081 taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1141 tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga tcacatggtc
     1201 ctgctggagt tcgtgaccgc cgccgggatc acttggggca tggacgagct gtacaagggc
     1261 ggtaccggag ggagcatggt gagaaagggc gaggagctgt tcaccggggt ggtgcccatc
     1321 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgggcgg cgagggtgag
     1381 ggcgatgcca ccgagggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     1441 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     1501 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacatccag
     1561 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     1621 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     1681 aacatcctgg ggcacaagct ggagtacaac accggagcag cagcacgctg gcgcgggcgg
     1741 gagcctaata gggtccaggc cctgcggcac ggcgtgcgcg tcctacgtgc agtggtcatc
     1801 gcctttgtgg tctgctggct gccctaccac gtgcggcgcc tcatgttctg ctacatctcg
     1861 gatgagcagt ggactccgtt cctctatgac ttctaccact acttctacat ggtgaccaac
     1921 gcactcttct acgtcagctc caccatcaac cccatcctgt acaacctcgt ctctgccaac
     1981 ttccgccaca tcttcctggc cacactggcc tgcctctgcc cggtgtggcg gcgcaggagg
     2041 aagaggccag ccttctcgag gaaggccgac agcgtgtcca gcaaccacac cctctccagc
     2101 aatgccaccc gcgagacgct gtactag
//
