LOCUS       sequence-406533-        2193 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent vasoactive intestinal 
            peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wang H, Qian T, Zhao Y, Zhuo Y, Wu C, Osakada T, Chen P, Chen Z, Ren
            H, Yan Y, Geng L, Fu S, Mei L, Li G, Wu L, Jiang Y, Qian W, Zhang L,
            Peng W, Xu M, Hu J, Jiang M, Chen L, Tang C, Zhu Y, Lin D, Zhou JN, 
            Li Y
  TITLE     A tool kit of highly selective and sensitive genetically encoded 
            neuropeptide sensors.
  JOURNAL   Science. 2023 Nov 17;382(6672):eabq8173. doi: 
            10.1126/science.abq8173. Epub 2023 Nov 17.
  PUBMED    37972184
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-11-17"; volume: "382(6672):eabq8173. doi"; pages: " 
            10.1126/science.abq8173. Epub 2023 Nov 17"
COMMENT     Sequence Label: pDisplay-GRAB_VIP1.0
FEATURES             Location/Qualifiers
     source          1..2193
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1293..1314
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1420..1441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1681..1700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtcgga cgctgctgcc tcccgcgctg
      121 ctgacctgct ggctgctcgc ccccgtgaac agcattcacc cagaatgccg atttcatctg
      181 gaaatacagg aggaagaaac aaaatgtgca gagcttctga ggtctcaaac agaaaaacac
      241 aaagcctgca gtggcgtctg ggacaacatc acgtgctggc ggcctgccaa tgtgggagag
      301 accgtcacgg tgccctgccc aaaagtcttc agcaattttt acagcaaagc aggaaacata
      361 agcaaaaact gtacgagtga cggatggtca gagacgttcc cagatttcgt cgatgcctgt
      421 ggctacagcg acccggagga tgagagcaag atcacgtttt atattctggt gaaggccatt
      481 tataccctgg gctacagtgt ctctctgatg tctcttgcaa caggaagcat aattctgtgc
      541 ctcttcagga agctgcactg caccaggaat tacatccacc tgaacctgtt cctgtccttc
      601 atcctgagag ccatctcagt gctggtcaag gacgacgttc tctactccag ctctggcacg
      661 ttgcactgcc ctgaccagcc atcctcctgg gtgggctgca agctgagcct ggtcttcctg
      721 cagtactgca tcatggccaa cttcttctgg ctgctggtgg aggggctcta cctccacacc
      781 ctcctggtgg ccatgctccc ccctagaagg tgcttcctgg cctacctcct gatcggatgg
      841 ggcctcccca ccgtctgcat cggtgcatgg actgcggcca ggctctactt agaagacacc
      901 ggttgctggg atacaaacga ccacagtgtg ccctggtggg tcatacgaat accgatttta
      961 atttccatca tcgtcaattt tgtccttttc attagtatta tacgaatttt gctgcagaag
     1021 ttaacatccc cacccgcgcc ggccgggccg cgcgacaccg acgcgctgga cctggaggag
     1081 ggaggaaacg tctatatcaa ggccgacaag cagaagaacg gcatcaaggc gaacttccac
     1141 atccgccaca acatcgagga cggcggcgtg cagctcgcct accactacca gcagaacacc
     1201 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcgt gcagtccaaa
     1261 ctttcgaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1321 gccgggatca ctctcggcat ggacgagctg tacaagggcg gtaccggagg gagcatggtg
     1381 agaaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1441 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
     1501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1561 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1621 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
     1681 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1801 gagtacaaca ccggagcagc agcacgctgg cgcgggtctc agtacaagag gctggccaag
     1861 tccacgctcc tgcttatccc gctgttcggc gtccactaca tggtgtttgc cgtgtttccc
     1921 atcagcatct cctccaaata ccagatactg tttgagctgt gcctcgggtc gttccagggc
     1981 ctggtggtgg ccgtcctcta ctgtttcctg aacagtgagg tgcagtgcga gctgaagcga
     2041 aaatggcgaa gccggtgccc gaccccgtcc gcgagccggg attacagggt ctgcggttcc
     2101 tccttctccc gcaacggctc ggagggcgcc ctgcagttcc accgcggctc ccgcgcccag
     2161 tccttcctgc aaacggagac ctcggtcatc tag
//
