LOCUS       sequence-406542-        1998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent norepinephrine (NE) 
            sensor GRAB_NE2m in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Feng J, Dong H, Lischinsky JE, Zhou J, Deng F, Zhuang C, Miao X, 
            Wang H, Li G, Cai R, Xie H, Cui G, Lin D, Li Y
  TITLE     Monitoring norepinephrine release in vivo using next-generation 
            GRAB(NE) sensors.
  JOURNAL   Neuron. 2024 Jun 19;112(12):1930-1942.e6. doi: 
            10.1016/j.neuron.2024.03.001. Epub 2024 Mar 27.
  PUBMED    38547869
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2024.03.001"; journalName: "Neuron"; date: 
            "2024-06-19- 19"; volume: "112"; issue: "12"; pages: "1930-1942.e6"
COMMENT     Sequence Label: pAAV-hsyn-NE2m
FEATURES             Location/Qualifiers
     source          1..1998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     protein_bind    64..88
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     1191..1212
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1318..1339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1579..1598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacacaagtt tgtacaaaaa agttggcacc accggtggct ccctgcagcc ggacgcgggc
      121 aacgcgagct ggaacgggac cgaggcgccg gggggcggcg cccgggccac cccttactcc
      181 ctgcaggtga cgctgacgct ggtgtgcctg gccggcctgc tcatgctgct caccgtgttc
      241 ggcaacgtgc tcgtcatcat cgccgtgttc acgagccgcg cgctcaaggc gccccaaaac
      301 ctcttcctgg tgtctctggc ctcggccgac atcctggtgg ccacgctcgt catccctttc
      361 tcgctggcca acgaggtcat gggctactgg tacttcggca aggcttggtg cgagatctac
      421 ctggcgctcg acgtgctctt ctgcacgtcg tccatcgtgc acctgtgcgc catcagcctg
      481 gaccgctact ggtccatcac acaggccaaa gagtacaacc tgaagcgcac gccgcgccgc
      541 atcaaggcca tcatcatcac cgtgtgggtc atctcggccg tcatctcctt cccgccgctc
      601 atctccatcg agaagaaggg cggcggcggc ggcccgcagc cggccgagcc gcgctgcgag
      661 atcaacgacc agaagtggta cgtcatctcg tcgtgcatcg gctccttctt cgctccctgc
      721 ctcatcatga tcctggtcta cgtgcgcatc taccagatcg ccaagcgtcg cacccgcgtg
      781 ccacccagcc gccggggtcc ggacgccgtc gccgcgccgc cggggggcac cgagcgcagg
      841 cccaacggtc tgggccccga gcgcagcgcg ggcccggggg gcgcagaggc cgaaccgctg
      901 cccacccagc tcaacggcgc ccctggcgag cccgcgccgg ccgggccgcg cgacaccgac
      961 gcgctggacc tggaggaggg aggaaacgtc tatatcaagg ccgacaagca gaagaacggc
     1021 atcaaggcga acttccacat ccgccacaac atcgaggacg gcggcgtgca gctcgcctac
     1081 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1141 ctgagcgtgc agtccaaact ttcgaaagac cccaacgaga agcgcgatca catggtcctg
     1201 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggcggt
     1261 accggaggga gcatggtgag aaagggcgag gagctgttca ccggggtggt gcccatcctg
     1321 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggtgagggc
     1381 gatgccaccg agggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1441 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1501 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta catccaggag
     1561 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1621 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1681 atcctggggc acaagctgga gtacaacacc ggagcagcag cacgctggcg cgggcggcag
     1741 aaccgcgaga agcgcttctt cttcgtgctg gccgtggtca tcggagtgtt cgtggtgtgc
     1801 tggttcccct tcttcttcac ctacacgctc acggccgtcg ggtgctccgt gccacgcacg
     1861 ctcttcaaat tcttcttctg gttcggctac tgcaacagct cgttgaaccc ggtcatctac
     1921 accatcttca accacgattt ccgccgcgcc ttcaagaaga tcctctgtcg gggggacagg
     1981 aagcggatcg tgttgtga
//
