LOCUS       sequence-406616-       10926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, COL3A1_CLU from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_COL3A1_CLU
FEATURES             Location/Qualifiers
     source          1..10926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(5687..6397,6398..6400,6401..6403))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5729..5750)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6078..6097
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6337..6358
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6400..6409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6485..6688)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6514..6534)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6689..6992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(7249..7293)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7478..7711
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8071..8188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8680..8805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8852..9109
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        9111..9490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9521..9625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9626..9694,9695..10486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9844..9863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10657..10926,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatt
     1801 cctctctgtg ttttttacga tactcctgca gtgctttttc ggccacggtt tccataaatt
     1861 tcgggttttt acggctaacc tcaaccggca cggtaacggt gatcgggtcg ctatcaaaca
     1921 gcttcaccac gacttccgtc acaccactcg gcacgtcgga gtcggacgtg tggctcgcta
     1981 ccgtagtaac acgcaggtaa tactgatcct ctccctgggt caggttggcc agacgactaa
     2041 cccaattaaa ctgttcattc agctgttcca acaggctgga ggtgttcagc attttccatt
     2101 ggtagctctt aagtagttca ttgtatttac gggtcagacg ttcggcgacc tgcaggcttt
     2161 catccagctc acgacgcagc ttggcctgtg acggattatt ggtgctgcaa tcaacgctca
     2221 gaatctcgcg gcacttatcg cattggtcct tcatgcgcag gcacccggtg ctgttatggc
     2281 ggatttcacg gcagacggtg cggtcgtcgt cgccctcacg aataaactcc gttggcggat
     2341 gctgaaacgc tgggctgtgg aagtggatat ccatagcttg ttgcgcttca tgaatcattt
     2401 ccagaaacgg ctgaaacatg gcgtgaaaat tcagcggttc ataagggctg aacggcatca
     2461 ggctacgaac aatacgggac ttcgggaaaa agaaatgcgg acggcggtgc ggcaagctga
     2521 acggcagata gtgatcgcca cggtctccct ttccacccgg cgagccgtca cgacccggca
     2581 aaccgtccga accagggtta ccgtcacggc ccggttcgcc cgccgtgccc gccaaacccg
     2641 gcaggccttg cgggcccggc ggaccgcgct ctccgctcag gccgttcgcg cccggcttcc
     2701 cagactctcc tttaacaccc tgtggtccag ggctaccacg cggacccggc atacccggag
     2761 gtcccgccag gccgcgagcc ccggtgatgc cggcaatacc taacggcccc ggcgcccccg
     2821 gtggaccctg ggcaccggga gatcccttct cacccggttg acccgcgtcg cctttcggac
     2881 cgcttacgcc cggcgaaccc ggggcaccgg tgtttcccgc tggtcctggg gggccatcct
     2941 tacccgggct accgcttggt ccaggtggac cagggttgcc gttagaaccc ggcggtcccg
     3001 gcaaaccacg cgcccctggg aaaccagccg cacccggacc acccggggaa ccgcgctcac
     3061 ccttcacacc ctgaggaccc ggcgggcccg ccgggccgga accacccggg ggaccggcga
     3121 cgcctggagg gccaccttcg cctttttcac cgggagcgcc acgctcaccc tttccgcctg
     3181 gctcaccatt ctgacccgga gcacccggga agcccgcagg tcccggcggg cccgtctctc
     3241 cgcgttcgcc cggtgaaccg cgcggcccgg cgatacccgg taaacccgga gcgccacctt
     3301 cgcctttgtc ccccggctga cccgcaggcc ccggcggacc gatgggaccg gtcggacccc
     3361 tgggaccgtc cttacccggc acgccatcag cccccggtcc gcccggctca cctttatcac
     3421 cttttgggcc cgggctgccc aacccaccac gttcgccagg cataccttgc aagcccggtg
     3481 taccagctgc gccaggcgga cctggcggac ccgctgcgcc cttgccacct tccggacccg
     3541 gaggccccgc gccgcctctc aaacccggcg caccagctaa gcccggcgga ccgcgctctc
     3601 ccggcgcacc cgcgtcgccc ttgccgcccg gcgcaccggg agcacctgcg tcgcccttcg
     3661 ggcccggctc gcccggcttc ccattctcgc ccggaggtcc accggtaccc ggcaagccct
     3721 gcaagccctg tggacccggc gggccggtgt cacctttgtc gccgccagga ccagttggac
     3781 ctggtggtcc ttgcggaccg gtttcgccgt tcttccccgg cggaccttgc ggtcccggac
     3841 cacccggacc gccgcgttca ccatttttac ccggggcgcc atcgttacct ttcgggcccg
     3901 ggaaacccat cacgccaggc tggccacgcg ggccgctcgg accgggtggg ccagggcgac
     3961 cggattcacc ctggctgcct ggaggacccg gctttccgtc cgaacccggg ccgcccggcg
     4021 atcccggcat tccacgcata ccgggaccac ccggaacgcc gtcacgtccc ggctcgcccg
     4081 ccgcaccacg tggaccggcg ggccccggtg caccgcgttc tccagcagga cccttctctc
     4141 ctggaatccc gttggggcca gccgggccgc ggaaacccgg tgcgccacgt tcacccgccg
     4201 ctcccggcag tccgttcgcg cccggctcac cgggactgcc atcctttcca tcttcccctt
     4261 tcgcgcccgg cacccccggg atgcctgcct caccccgttc accacgcgga cccggctcgc
     4321 ctttcgcgcc gttcttaccc ggctctcccg ccccgccccg caggcctggc gcaccattcg
     4381 cgcccgccgg accgggcggg ccacgagcac ccataagccc cggcgcgccc gggatgccgg
     4441 cagggcccat ctcgcccttc ccgcccgggc taccgttaat gcccggcggt cccggtggac
     4501 cctgtgcgcc ggcatgacct tgcggacctg gctcgccacg ttgcccgggc gcaccattag
     4561 aacccggcga acccgcggga ccaacctcac ctttagcccc cggagacccc gggaaacccg
     4621 cggtacccgg tggacctggc ggaccaggct gaccgtcgga accacgcgcg ccatcattgc
     4681 cgcgtgcacc tgccgcacca gggaggccag gacgtccccg ttcaccgggc gctcctctcg
     4741 gccccatagg gcccggtgcg ccgttctcac ccggaaggcc gttttcacct tttaagcccg
     4801 gcgcgccagt ctcacctttt tcaccgttgc gcccatcgaa accacggtgg cctttcatgc
     4861 ccgggaaacc cgggatacct gccgggcctt taatcccggg cgggccgggc agtcctctct
     4921 cacccggacg acctggacgg ccgctctccc catccttgcc ggcagggccg gaaggaccaa
     4981 tcgcccccgg cgggcctggc gggccgctcg ggcccgcttg gccgggctca cccggaggac
     5041 cctggtaacc cggagagccc ggggagcccg ggtgacctga ggtgccaggc ggacctggtg
     5101 gccccggcgg gcccgccgga cccggataac ctgcgagccc acccaccgcg acgccgcttt
     5161 tcacatcgta agagtcgtac tgcgggctat agttctgcgg gcccgtcgga caggattcgc
     5221 atatacccgg cggacccggc gacccagggc tacccggttg gccaggaata cccggatcgc
     5281 cattacgacc ggggatgcct ggcggtcccg ggtcgccctt ggggccctgc ggaccctgac
     5341 cgttcggcgg acgcgttggc gcggtcggcg gttgcgggca aaccgcgcag cactcaccaa
     5401 atgggatctc cgggttcgga caatccagct cttggtcatc gcagatgatg tcatcacaca
     5461 gcacggaccc gctgtcgcaa acacagatct gacacggctc cggtttccaa acatcgcgat
     5521 ctgcgtagct ttgacccagg tggctgcaac caccttcaac tgcttcttgc tgtgccagga
     5581 tgatggtcgg atgcagcaga gccagcagca accaacttcc tttttgtaca aatgacatca
     5641 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     5701 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     5761 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     5821 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     5881 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     5941 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     6001 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     6061 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     6121 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     6181 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     6241 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     6301 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     6361 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     6421 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     6481 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     6541 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     6601 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     6661 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     6721 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     6781 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     6841 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     6901 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     6961 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7021 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     7081 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     7141 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     7201 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     7261 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     7321 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     7381 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     7441 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     7501 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     7561 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     7621 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     7681 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     7741 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     7801 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     7861 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     7921 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     7981 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     8041 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     8101 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     8161 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
     8221 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
     8281 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
     8341 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     8401 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     8461 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     8521 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
     8581 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     8641 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     8701 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     8761 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     8821 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     8881 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     8941 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     9001 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     9061 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
     9121 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     9181 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     9241 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     9301 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     9361 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     9421 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     9481 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9781 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9841 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9901 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9961 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10021 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10081 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10141 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10201 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    10261 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10321 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10381 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10441 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10801 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10921 taccgg
//
