LOCUS       sequence-406618-        8433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, CREBBP_ATG10 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_CREBBP_ATG10
FEATURES             Location/Qualifiers
     source          1..8433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3194..3904,3905..3907,3908..3910))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3236..3257)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3585..3604
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3844..3865
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3907..3916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3992..4195)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4021..4041)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4196..4499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4756..4800)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4985..5218
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5578..5695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6187..6312
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6359..6616
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6618..6997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7028..7132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7133..7201,7202..7993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7351..7370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8164..8433,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 gtacgttacg ctcatcttgg cttgtagctt tcgcgtaaga cagaggaagg ttcaaaccga
     1861 cgacggggcc tacaatagat aaccatgatg tgatgtagtt tacattttta ttaatctttt
     1921 gagagttctt cagaacggga gtcataaatt cgttagtttt gcacggatga aggacaaaga
     1981 aaggttgccc caaaataggg tgttcctgct gggtaatggt atcccaaggg ccttgtaata
     2041 aacgcatctt atagcactca tgcacaccct cccagatgtc cttaagagtc aatgggcgcc
     2101 catccagaaa ggatgcacgg aaatataaaa cgggtacttg gtatgagcat gagtacagaa
     2161 catggtactc gtatttgatt acctctgagg ccgcagcagt ctcaattacc tcgcagtcgt
     2221 ctaaaggcag ttcaaacgcc tcctccatag gaagacacgt ttgtccatga gtcgaggcac
     2281 ccaggtgact cataacggac ccgttcttga tttgaaaatg gattttacac atgtacccgt
     2341 cggagcagtc cttagccatt cctccagctt gggcagtatt cagaccagca tgacctgtca
     2401 tttgaggaga tacctgagtt aaggtctctg ccaggacaga gcttgaggct ccttgcatgg
     2461 ctggtgttgg atatggcatg cctgccccac gtccgcgacc tgcagcaccc aatgacccat
     2521 tcataacttg ggcttggcct tgagatgcct gattgatcaa gctatgcccg gagttcgagt
     2581 ttaacagtcc aggatgtgtt tggttgaagt ttgcgttcac acaaatgccg ggaccagttt
     2641 gtgacgttgc tggcgaagac gtagccagtc caacctgctt ttgcgcctgt ggattaagtg
     2701 cttggctcgc cgcgggtgtt gggccggacg tactggctgc ctgctttggc agcgagggag
     2761 cactactgtc gccctgactt aaaggacttt ttcccattgc gctaaggcta gccatattcg
     2821 cagaattagg ttgtccttga gcctgccccc ctaagccttg ttggacgggc gaggacgcag
     2881 atacgttccc aatgccaggg ttaattgaag aaccactacc tccacgcagt aactcggata
     2941 actgtttgtg ttttgatgct gcgtcaggaa caaggttacc cgaatttaaa agccctaact
     3001 cgccgccatt tggaataagc tcgtcaggca gatcgttttc taagtcgaac aaagagccaa
     3061 aatcggttga atcgtttgcc gaaaaaccgg gcgaagacaa tttagcgcgt ttcgggtttg
     3121 gcggtccgtc aagtaagttc tctgccattc cccgggaagc ggccgcactt cccccggagc
     3181 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     3241 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3301 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     3361 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     3421 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     3481 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     3541 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     3601 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     3661 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     3721 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     3781 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     3841 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     3901 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     3961 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     4021 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     4081 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     4141 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     4201 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     4261 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     4321 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     4381 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     4441 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     4501 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     4561 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     4621 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     4681 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     4741 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     4801 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     4861 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     4921 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     4981 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     5041 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     5101 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     5161 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     5221 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     5281 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     5341 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
     5401 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
     5461 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     5521 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
     5581 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     5641 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     5701 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     5761 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
     5821 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
     5881 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     5941 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     6001 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
     6061 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     6121 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     6181 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     6241 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     6301 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     6361 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     6421 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     6481 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     6541 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     6601 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     6661 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     6721 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     6781 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     6841 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     6901 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     6961 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     7021 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7081 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7141 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7201 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7261 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7321 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7381 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7441 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     7501 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     7561 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     7621 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     7681 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     7741 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7801 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7861 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7921 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7981 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8041 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8101 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8161 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8221 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8281 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8341 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8401 tgctgccagt ggcgataagt cgtgtcttac cgg
//
