LOCUS       sequence-406756-       12992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with a E1686R mutation to 
            enhance expression and an exofacial double HA tag in domain II .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.1 HA pcDNA3
FEATURES             Location/Qualifiers
     source          1..12992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2703..2729
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2733..2759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7536..7565
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(8545..8563)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8546..8563)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8583..8600)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8589..8813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8859..9287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8946..8965)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9156..9177
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9296..9316)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9301..9630
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9481..9616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9543..9562
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9697..10491
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9751..9770)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10361..10380
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10665..10786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10702..10721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10756..10775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10835..10851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10835..10851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10848..10870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10859..10875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10883..10889,10890..10907,10908..10913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10928..10949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11066..11083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11237..11825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11317..11336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11996..12787,12788..12856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12619..12638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12857..12961)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
      961 ctggcggccg ctcgaggtct tgcagaatgg cccgctttgg agacgagatg ccgggccgct
     1021 acggcgcagg cggaggaggc tcagggccgg ccgccggggt ggtcgtgggc gccgcgggcg
     1081 gccgaggagc cgggggcagc cggcagggcg ggcagcccgg agcgcagagg atgtacaagc
     1141 agtcgatggc gcagagagcg cggaccatgg ccctctacaa ccccatccct gtccgccaga
     1201 actgcctcac ggtcaaccgc tccctgttcc tcttcagtga agacaacgtg gtgagaaaat
     1261 acgccaaaaa gatcacggaa tggcctccct tcgagtacat gatcctggcc accatcattg
     1321 ctaactgcat cgtcctggcc ctggagcagc acctccctga tgatgacaag acacccatgt
     1381 ccgagcggct ggatgacaca gaaccctatt tcattggcat cttctgtttt gaggctggaa
     1441 ttaagatcgt ggctcttggc tttgccttcc acaaaggctc ctacctgagg aatggctgga
     1501 acgtcatgga ctttgtcgtg gtgctaacag gcatcttggc cactgtcggg acggagtttg
     1561 atctacggac actgagggcg gttcgtgtgc tgcggccact caagctggtg tctggaatcc
     1621 caagtttaca agtcgtcctg aagtcaatca tgaaggcgat gatccctctg ctgcagatcg
     1681 gcctcctcct gttttttgca atccttattt ttgcaatcat agggttagaa ttttatatgg
     1741 gaaaatttca taccacctgc tttgaagagg ggacagacga catccagggt gagtcgccag
     1801 ctccgtgtgg gacagaggag cctgcccgca cctgccccaa cgggaccaaa tgtcagccgt
     1861 actgggaagg gcccaacaac ggcatcactc agttcgacaa catcctgttt gctgtgctca
     1921 ctgttttcca gtgcatcacc atggaaggct ggactgatct cctctacaat agcaacgatg
     1981 cctcagggaa cacttggaac tggttgtact tcatccccct catcatcatc ggctcctttt
     2041 ttatgctgaa ccttgtgctg ggtgtgctgt ctggggagtt tgccaaagaa agggaacgtg
     2101 tagagaaccg aagggctttt ctgaagctca gaagacaaca gcagattgaa cgtgagctca
     2161 atggatacat ggagtggatc tcgaaagcag aagaggtgat cctcgcggag gacgaaacag
     2221 acgtggagca gaggcaccct tttgatggag ctcttcggag agctactctg aagaaaagca
     2281 agacggacct gctcaaccct gaggaggcgg aggaccagct tgctgacatc gcgtctgtgg
     2341 ggtctccctt cgccagagcc agcatcaaaa gtgccaagct ggagaattcg acttttttcc
     2401 acaaaaagga gagaagaatg cgtttctaca tccgccgcat ggtcaaaact caggccttct
     2461 actggaccgt gctcagtctg gtagccctca acacgctgtg cgtcgccatt gtccactaca
     2521 accagcccga gtggctctcc gacttcctct actatgcaga attcattttc ttaggactct
     2581 ttatgtccga aatgtttata aaaatgtatg ggctcgggac acggccttac ttccactctt
     2641 ccttcaactg ctttgactgt ggggtcatca tcgggagcat ctttgaagtc atctgggccg
     2701 tctaccccta cgacgtcccc gactacgccg gctacccgta cgacgtcccg gactacgccg
     2761 gcgtcgacat caaaccgggt acatcctttg gaatcagcgt gttacgagct ctcaggttac
     2821 tgcgtatttt caaagtcaca aagtactggg catctctcag aaacctggtt gtctccctcc
     2881 tcaactccat gaaatccatc ataagtctgc tgttcctcct cttcctcttc attgtcgtct
     2941 ttgccctctt ggggatgcag ctgtttggtg gccagtttaa ttttgacgag gggactcctc
     3001 ccaccaactt cgacactttt ccagcagcaa taatgactgt gtttcagatc ctgactggcg
     3061 aggattggaa tgaggtcatg tatgatggga tcaagtctca ggggggcgtg cagggcggca
     3121 tggtgttctc catctacttc atcgtcctca ccctcttcgg gaactacacc ctgctgaacg
     3181 tgttcttggc tatcgcggtg gacaacctgg ccaacgccca ggaactcacc aaggatgaac
     3241 aagaagagga agaggcagcc aatcagaaac tggctctaca gaaagccaag gaggtggcag
     3301 aagtgagtcc cctgtctgca gccaacatgt ccatagctgt gaaggaacag cagaagaacc
     3361 agaagcctgc caagtcggtg tgggagcagc gcaccagcga gatgcgcaag cagaacctgc
     3421 tggctagccg cgaggcgctg tacggggacg cggctgagcg ctggcccacc acttacgcgc
     3481 gcccgctgcg gccggacgtg aagacgcact tggaccggcc gctcgtggtg gacccgcagg
     3541 agaaccgtaa caacaacacc aacaagagcc gtgcgccaga agcgctgcgc caaaccgcgc
     3601 ggccccgcga gagcgcgcgc gaccccgacg cgcggcgcgc ctggcccagc agccctgagc
     3661 gcgcccctgg acgagagggc ccgtatggcc gcgagagcga gccgcaacag cgcgagcacg
     3721 cgccaccccg cgagcacgta ccctgggacg cggatcctga gcgcgccaag gccggggacg
     3781 cgccccgccg ccacacgcac cggcctgtgg ccgagggcga gcctcgtcgc caccgcgcgc
     3841 gccgccggcc cggggacgaa ccggacgaca gaccggagcg caggccgcgt ccccgcgacg
     3901 ccactaggcc ggcccgcgct gcagacggcg aaggcgatga tggggagcgc aagcggcgac
     3961 accgacacgg gccgccggcc cacgatgaca gggagcgcag acaccggcgc agaaaagaga
     4021 gccagggctc tggggtcccc atgtctggtc ccaacctgtc caccaccagg ccaatccagc
     4081 aggatctggg ccgccaggac ctgccactgg ctgaggacct ggacaacatg aagaacaaca
     4141 agttggccac cggggagcct gccagtcccc acgacagcct gggccacagt ggccttcccc
     4201 ctagccctgc caagatcggg aacagcacca accctggtcc cgccttggcc accaatcccc
     4261 agaatgctgc cagccgcagg acgcccaaca acccgggcaa cccgtccaac cccggccccc
     4321 ccaagactcc cgagaacagc cttatcgtca ccaaccccag cagcacccag cccaactcag
     4381 caaagactgc caggaaaccc gagcacatgg cggtggagat ccccccggcc tgcccgcccc
     4441 tcaaccacac tgtggtccaa gtaaacaaaa acgccaaccc agacccactg ccaaagaaag
     4501 aggaagagaa gaaggaggaa gaggaggcag accccgggga ggatggccca aagcccatgc
     4561 cgccctacag ctccatgttc atcctctcca ccaccaaccc ccttcgccgg ctgtgccatt
     4621 acatcctgaa cctgcgctac ttcgagatgt gcatcctcat ggtcattgcc atgagtagca
     4681 tcgcgctggc cgccgaggac ccggtgcagc ccaacgcacc ccgcaacaac gtgctgcgat
     4741 attttgacta tgttttcaca ggagtgttta cctttgagat ggtgatcaag atgatcgacc
     4801 tgggcctcgt cctgcatcag ggggcctatt tccgtgacct gtggaacatt ctggacttca
     4861 tagtggtcag tggggccctg gtggcctttg ccttcactgg caatagcaaa ggaaaggaca
     4921 tcaacaccat caagtccctc cgagtcctcc gggtgctacg acctctaaag accatcaagc
     4981 ggctgcctaa gttgaaggcc gtatttgact gcgtggtgaa ctcgctcaag aacgtcttca
     5041 acatcctcat tgtctacatg ctcttcatgt tcatcttcgc cgtggtggcc gtgcagctct
     5101 tcaagggcaa attcttccac tgcacggacg agtccaagga gtttgagaga gactgtcgag
     5161 gcaaatacct cctttacgag aagaacgagg taaaggcgcg ggaccgcgag tggaagaaat
     5221 acgacttcca ctacgacaac gtgctctggg ccctgctcac gctctttacg gtgtccacgg
     5281 gagagggctg gccacaggtc ctcaagcact cagtggatgc cacttttgag aaccagggcc
     5341 ccagccccgg gtaccgcatg gaaatgtcca tcttctacgt ggtctacttt gtggtgtttc
     5401 ccttcttctt tgtcaatatc tttgtggcct tgatcatcat caccttccag gagcagggag
     5461 acaagatgat ggaagaatac agcctagaga aaaatgagag ggcctgcatc gactttgcca
     5521 tcagtgccaa gccgctgacc aggcacatgc cccagaacaa gcagagcttc cagtatcgaa
     5581 tgtggcagtt cgtggtgtcc ccaccctttg agtacaccat catggccatg atcgctctca
     5641 acaccatcgt gctaatgatg aagttctatg gagcctctgt ggcctatgaa aacgcccttc
     5701 gagtgttcaa cattgtcttc acctccctct tctctctcga atgtgtgctc aaagtcatgg
     5761 cttttgggat tctgaattat ttccgcgatg cctggaacat cttcgacttt gtgactgttc
     5821 tgggcagcat cacagacatc ctcgtcaccg agtttgggaa taacttcatc aacctgagct
     5881 ttctccgcct cttccgtgct gcccgactca tcaaactcct ccgccagggt tacaccatcc
     5941 gcattctcct ctggactttc gtgcagtctt tcaaggccct accttatgtc tgtctgctga
     6001 tcgccatgct cttcttcatc tatgccatca tcgggatgca ggtgtttggc aacatcggca
     6061 ttgatgggga agatgaggac agcgatgagg atgagttcca aatcacgagg cacaataact
     6121 tccggacctt cttccaagct ctcatgcttc tcttccggag cgccacaggg gaagcgtggc
     6181 acaacatcat gctgtcctgc ctcagcggga agccatgcga caagaactcc gggatccaaa
     6241 aaccagagtg tggcaacgag ttcgcctatt tttactttgt ctcgttcatc ttcctttgct
     6301 catttctgat gctgaatctc tttgttgctg tcatcatgga caacttcgag tacctcaccc
     6361 gagattcctc catcctgggc ccccaccacc tggatgagta cgtgcgtgtc tgggcagagt
     6421 atgaccctgc tgcctgcggc cgcattcact ataaggacat gtacagttta ttgcgagtaa
     6481 tatcgccccc tctcggctta ggcaagaaat gtcctcatag ggttgcttgc aagaggctct
     6541 tgcggatgga cctacccgta gcggatgaca acaccgttca cttcaactcc accttgatgg
     6601 ctctgatccg aaccgccctg gatatcaaaa tcgcaaaggg tggagctgac aagcagcaaa
     6661 tggacgcaga gctccgcaag gaaatgatgg ccatttggcc caacctgtct cagaagacct
     6721 tggatctgct ggtcacacct cacaagtcca cggacctgac agtgggtaag atctacgcag
     6781 ccatgatgat catggagtac taccggcaga gcaaggccaa gaagctgcag gccatgcgag
     6841 aggagcagaa ccggacacca ctcatgttcc agcgcatgga gcctccatcg ccaacacagg
     6901 agggaggacc cagccaaaac gcccttccct ccactcagct ggaccccgga ggaggcctga
     6961 tggctcaaga aagcagcatg aaggagagcc cgtcctgggt gacccagcgg gcacaggaga
     7021 tgttccagaa gactggtacc tggagcccag agcgagggcc acccatcgac atgcctaaca
     7081 gccagcccaa ctcccagtct gtggagatga gagaaatggg aactgatggc tactcagaca
     7141 gcgaacacta cctccccatg gaaggacaga ccagggccgc ctccatgccc cgcctcccag
     7201 cagagaacca gaggagaagg ggccggccac gtggaaataa cctcagtacc atctctgata
     7261 ccagccccat gaagcgctca gcctccgtgc tgggacccaa agcccggcga ctggatgact
     7321 actcactaga gcgggtacca cctgaggaga accaaaggta ccaccaacgc cgccgggacc
     7381 gtggccaccg cacctctgag cgctctctgg gccgatacac tgatgtggac acaggcctgg
     7441 ggacagatct gagcatgacc acccaatcgg gtgacctgcc ctccaaagat cgggaccagg
     7501 accggggccg gcccaaggac cggaagcatc ggccacacca ccaccaccac catcatcacc
     7561 atcatccccc ggccccggac cgggagcgct acgcacagga gcggccggac accggccggg
     7621 cgcgggcccg ggagcagcgc tggtcccgct cgcccagcga gggtcgggag cacgcgacac
     7681 acagacagta gttccgtcag tggaagccca gccccctcta cgtccggcac cagcacgccg
     7741 cggcgcggcc gccgccagct cccccagacc ccctgcaccc cgcggccgct tgtgtcctac
     7801 tcgcccgctc cgcgcaggcc tgcggcgcgc aggatggcgg gtcccccagc gccccctggc
     7861 ggctcgccgc ggggttgccg ccgtgcgccg cgctggcctg cgcatgcgcc tgagggcccg
     7921 cggccgcgcg gtgccgacta caccgagccc gacagcccgc gcgagccgcc agggggcgcg
     7981 catgagcccg cgccgcggtc gcccaggact ccgcgcgccg cgggctgcgc gtcaccgcgc
     8041 cacggccgca ggctgcccaa tggttactac gcggggcacg gcgcgccacg gccgcgcaca
     8101 gcccgcaggg gcgcgcacga cgcgtacagc gagagcgagg acgactggtg ctaagccgcc
     8161 ccgccccccg cagcgcccgt acggtgcggg cgcaccgcaa atgataaaca ataacgtttt
     8221 taaaaaagaa aaccggggag aaaaacaaaa atagcttcta ttgatgagtt ttatcatctc
     8281 gattgaatct ttcgttttcc cggtgaacga aaccgaggtg gctgcgagcc ggagggacgg
     8341 ctgcacgccc cacacacttc tcagacccac gggagttctt gcgggccacg ccaaacttat
     8401 tactgcctgc agagttcaac tacaagaaaa taacactaac agtttttaaa aggactccgt
     8461 aaaaagaaat ttgaaagaga atgtagagag gctgagaaag cccaggaatt cctgcagccc
     8521 gggggatcca ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     8581 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     8641 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8701 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8761 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     8821 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8881 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8941 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     9001 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     9061 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9121 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9181 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     9241 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     9301 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9361 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9421 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     9481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9541 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     9601 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     9661 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     9721 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9781 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9841 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9901 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9961 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
    10021 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
    10081 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
    10141 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
    10201 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
    10261 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
    10321 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    10381 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    10441 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
    10501 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
    10561 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    10621 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    10681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    10801 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10981 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    11041 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    11101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    11161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    11221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    11281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    11341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    11401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    11461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    11521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    11581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11641 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    11701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11881 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11941 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    12001 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    12061 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    12121 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    12181 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    12241 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    12301 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    12361 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    12421 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    12481 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    12541 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12601 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12661 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12721 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12781 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12841 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12901 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12961 gcacatttcc ccgaaaagtg ccacctgacg tc
//
