LOCUS       sequence-407158-       12185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal TurboID tagged human STING for proximity 
            ligation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12185)
  AUTHORS   Gentili M, Liu B, Papanastasiou M, Dele-Oni D, Schwartz MA, Carlson 
            RJ, Al'Khafaji AM, Krug K, Brown A, Doench JG, Carr SA, Hacohen N
  TITLE     ESCRT-dependent STING degradation inhibits steady-state and 
            cGAMP-induced signalling.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):611. doi: 10.1038/s41467-023-36132-9.
  PUBMED    36739287
REFERENCE   2  (bases 1 to 12185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):611. doi"; pages: " 
            10.1038/s41467-023-36132-9"
COMMENT     Sequence Label: pTRIP-hPGK-Blast-2A-STING-TurboID
FEATURES             Location/Qualifiers
     source          1..12185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          6..71
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             201..221
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    646..780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(683..702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     737..756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             948..1581
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1628..1753
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2246..2479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2664..2708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3001..3118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3166..3676
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3595..3613
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      3689..3698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3695..4090
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4106..4162
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5318..6274
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     LTR             6552..6732
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8260..8279)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8493..8512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8612..8634
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8672..8690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8758..8862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8863..8931,8932..9723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9894..10482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10383..10402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10636..10653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10723..10743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10728..11057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10908..11043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
       61 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
      121 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
      181 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
      241 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
      301 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
      361 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
      421 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
      481 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
      541 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      601 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      781 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
      841 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
      901 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
      961 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     1021 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     1081 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     1141 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     1201 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     1261 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     1321 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     1381 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     1441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     1501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     1561 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     1621 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     1681 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1741 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1801 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1861 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1921 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1981 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     2041 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     2101 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     2161 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     2221 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     2281 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     2341 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     2401 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     2461 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     2521 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     2581 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     2641 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     2701 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2761 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2821 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2881 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2941 gattagtgaa cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa
     3001 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     3061 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     3121 ggtttattac agggacagca gagatccact ttggctgata cgcgtggggt tggggttgcg
     3181 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     3241 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     3301 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     3361 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     3421 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     3481 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     3541 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3601 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3661 cgacctctct ccccagccgc gggctagcgc caccatggcc aagcctttgt ctcaagaaga
     3721 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     3781 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     3841 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     3901 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     3961 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     4021 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     4081 gtgggagggc cgtacgggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     4141 cgtggaggag aaccctggac ctggatccat gccccactcc agcctgcatc catccatccc
     4201 gtgtcccagg ggtcacgggg cccagaaggc agccttggtt ctgctgagtg cctgcctggt
     4261 gaccctttgg gggctaggag agccaccaga gcacactctc cggtacctgg tgctccacct
     4321 agcctccctg cagctgggac tgctgttaaa cggggtctgc agcctggctg aggagctgcg
     4381 ccacatccac tccaggtacc ggggcagcta ctggaggact gtgcgggcct gcctgggctg
     4441 ccccctccgc cgtggggccc tgttgctgct gtccatctat ttctactact ccctcccaaa
     4501 tgcggtcggc ccgcccttca cttggatgct tgccctcctg ggcctctcgc aggcactgaa
     4561 catcctcctg ggcctcaagg gcctggcccc agctgagatc tctgcagtgt gtgaaaaagg
     4621 gaatttcaac gtggcccatg ggctggcatg gtcatattac atcggatatc tgcggctgat
     4681 cctgccagag ctccaggccc ggattcgaac ttacaatcag cattacaaca acctgctacg
     4741 gggtgcagtg agccagcggc tgtatattct cctcccattg gactgtgggg tgcctgataa
     4801 cctgagtatg gctgacccca acattcgctt cctggataaa ctgccccagc agaccggtga
     4861 ccgtgctggc atcaaggatc gggtttacag caacagcatc tatgagcttc tggagaacgg
     4921 gcagcgggcg ggcacctgtg tcctggagta cgccaccccc ttgcagactt tgtttgccat
     4981 gtcacaatac agtcaagctg gctttagccg ggaggatagg cttgagcagg ccaaactctt
     5041 ctgccggaca cttgaggaca tcctggcaga tgcccctgag tctcagaaca actgccgcct
     5101 cattgcctac caggaacctg cagatgacag cagcttctcg ctgtcccagg aggttctccg
     5161 gcacctgcgg caggaggaaa aggaagaggt tactgtgggc agcttgaaga cctcagcggt
     5221 gcccagtacc tccacgatgt cccaagagcc tgagctcctc atcagtggaa tggaaaagcc
     5281 cctccctctc cgcacggatt tctcttctag agctagcaaa gacaatactg tgcctctgaa
     5341 gctgatcgct ctcctggcta atggcgagtt ccatagtggc gaacagctgg gagaaaccct
     5401 gggcatgtcc agggccgcta tcaacaagca cattcagact ctgcgcgact ggggcgtgga
     5461 cgtgttcacc gtgcccggaa agggctactc tctgcccgag cctatcccgc tgctgaacgc
     5521 taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg tggtcgactc
     5581 caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg cttgcattgc
     5641 agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc cttttggagc
     5701 taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa tcggcctggg
     5761 cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg
     5821 agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca tcctggtgga
     5881 gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc
     5941 tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc aggaagcagg
     6001 gattaacctg gacaggaata ctctggccgc tacgctgatc cgagagctgc gggcagccct
     6061 ggaactgttc gagcaggaag gcctggctcc atatctgcca cggtgggaga agctggataa
     6121 cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg ggattagccg
     6181 ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca aaccctggat
     6241 gggcggagaa atcagtctgc ggtctgccga aaagctgcag taactcgaga cctagaaaaa
     6301 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     6361 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     6421 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6481 ctaattcact cccaacgaag acaaaatcgt cgagagatgc tgcatataag cagctgcttt
     6541 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6721 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6781 aatgaatatc agagagtgag aggccttgac attataatag atttagcagg aattgaacta
     6841 ggagtggagc acacaggcaa agctgcagaa gtacttggaa gaagccacca gagatactca
     6901 cgattctgca catacctggc taatcccaga tcctaaggat tacattaagt ttactaacat
     6961 ttatataatg atttatagtt taaagtataa acttatctaa tttactattc tgacagatat
     7021 taattaatcc tcaaatatca taagagatga ttactattat ccccatttaa cacaagagga
     7081 aactgagagg gaaagatgtt gaagtaattt tcccacaatt acagcatccg ttagttacga
     7141 ctctatgatc ttctgacaca aattccattt actcctcacc ctatgactca gtcgaatata
     7201 tcaaagttat ggacattatg ctaagtaaca aattaccctt ttatatagta aatactgagt
     7261 agattgagag aagaaattgt ttgcaaacct gaatagcttc aagaagaaga gaagtgagga
     7321 taagaataac agttgtcatt taacaagttt taacaagtaa cttggttaga aagggattca
     7381 aatgcataaa gcaagggata aatttttctg gcaacaagac tatacaatat aaccttaaat
     7441 atgacttcaa ataattgttg gaacttgata aaactaatta aatattattg aagattatca
     7501 atattataaa tgtaatttac ttttaaaaag ggaacataga aatgtgtatc attagagtag
     7561 aaaacaatcc ttattatcac aatttgtcaa aacaagtttg ttattaacac aagtagaata
     7621 ctgcattcaa ttaagttgac tgcagatttt gtgttttgtt aaaattagaa agagataaca
     7681 acaatttgaa ttattgaaag taacatgtaa atagttctac atacgttctt ttgacatctt
     7741 gttcaatcat tgatcgaagt tctttatctt ggaagaattt gttccaaaga ctctgaaata
     7801 aggaaaacaa tctattatat agtctcacac ctttgtttta cttttagtga tttcaattta
     7861 ataatgtaaa tggttaaaat ttattcttct ctgagatcat ttcacattgc agatagaaaa
     7921 cctgagactg gggtaatttt tattaaaatc taatttaatc tcagaaacac atctttattc
     7981 taacatcaat ttttccagtt tgatattatc atataaagtc agccttcctc atctgcaggt
     8041 tccacaacaa aaatccaacc aactgtggat caaaaatatt gggaaaaaat taaaaatagc
     8101 aatacaacaa taaaaaaata caaatcagaa aaacagcaca gtataacaac tttatttagc
     8161 atttacaatc tattaggtat tataagtaat ctagccagat cctctacgcc ggacgcatcg
     8221 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     8281 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     8341 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     8401 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     8461 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8521 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8581 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     8641 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8701 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     8761 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8821 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8881 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8941 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9001 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9061 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9121 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9181 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9241 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9301 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9361 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9421 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9481 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9541 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9601 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9661 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9721 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9781 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9841 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9901 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9961 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10021 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10201 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10261 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10501 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10561 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10621 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
    10741 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    10801 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10861 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
    10921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    11041 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
    11101 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
    11161 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
    11221 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
    11281 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
    11341 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
    11401 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
    11461 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
    11521 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
    11581 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
    11641 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
    11701 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
    11761 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
    11821 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
    11881 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
    11941 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
    12001 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
    12061 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
    12121 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
    12181 aagct
//
