LOCUS       sequence-407178-        9581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VEGFR2 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-VEGFR2-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3777..3796)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3778..3796)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3841..4044)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3870..3890)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4045..4424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4596..4615
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4690..4794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4795..4863,4864..5655)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5013..5032)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5826..6414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6315..6334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6568..6585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6702..6723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6738..6743,6744..6761,6762..6768)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6776..6792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6781..6803
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6800..6816
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6800..6816
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6865..6986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6876..6895)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6930..6949
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7162..7956)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7273..7292)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7883..7902
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8023..8352)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8037..8172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8091..8110)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8337..8357
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8366..8794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8476..8497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8688..8707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8840..9064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9053..9070
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(9093..9110)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9120..9161)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ttcaaatgtt tttacactca caggccggct ctttcgctta ctgttctgca gatactgact
       61 gattcctgct gtgttgtcat aatggaattt ggggtcacat acttcctcct cctccataca
      121 ggaaacaggt gaggtaggca gagagagtcc agaatcctct tccatgctca aagtctctga
      181 tatcggaaga acaatgtagt ctttgccatc ctgctgagca ttagcttgca agagatttcc
      241 caaatgttcc accaactctg aaaacgtggg tctctgactg ggctccccgt gccagcagtc
      301 cagcatggtc tggtacattt ctggtgtagt ataatcaggg gccctcattc tagttccttc
      361 tttcaatcgc ctacaaaatt cttcatcaat ctttacccca ggatatggag aagcacctaa
      421 ggaaaatatt tcccacagca aaacaccaaa agaccagacg tcactctgga ttgtgtacac
      481 tctgtcaaaa attgtttctg gggccatcca tttcaaaggg aggcgagcat ctccttttct
      541 gacataatct ggatctttat aaatatcccg ggccaagcca aagtcacaga ttttaaccac
      601 gttcttctcc gataagagga tatttcgtgc cgccaggtcc ctgtggatac actttcgcga
      661 tgccaagaac tccatgccct tagccacttg gaagctgtaa cagatgagat gctccaaggt
      721 caggaagtcc ttatacagat cttcaggagc ttcctcttct tctacatcac tgagggactt
      781 ctcctccaca aatccagagc tggctgagct ctggctactg gtgatgctgt ccaagcgccg
      841 tttcagatcc acagggattg ctccaacgta gtctttccct tgacggaatc gtgccccttt
      901 ggtcttgtag gggacaaatt catttctctt gctcctcagg taagtggaca ggtttccaaa
      961 tttgcagaat tccacaatca ccatgagtgg ccctcctggc ttggtacagg cacctagaag
     1021 gttgaccaca ttgagatggt gaccaatatg aatgaggatc ttgagttcag acatgagagc
     1081 tcgatgctca ctgtgtgttg ctccttcttt caacattttg actgctactg tcctgcaagt
     1141 tgctgtcttg tcaattccaa aggcatctgc ttcaatcact tggccaaagg caccacggcc
     1201 aagaggctta cctagcttca gccggtctct ggggaattcc catttgctgg catcataagg
     1261 cagtcgttca caatgttcat ccaatgggag ttcatctgga tccatgacga tggacaagta
     1321 gcctgtcttc agttcccctc cattggcccg cttaacggtc cgtaggatga tgacaagaag
     1381 tagccagaag aacatggcaa tcaccgccgt gcctactaga ataatgattt ccaagttcgt
     1441 cttttcctgg gcaccttcta ttatgaaaaa tgcctccact tttgcacagc caagaacact
     1501 gcatgcctgg caggtgtaga ggccttcgtc ctccttcctc actctgcgga tagtgaggtt
     1561 ccggttccca tccttcaata caatgcctga gtcttctaca agggtctcat tatctttaaa
     1621 ccacatgatc tgtggagggg gattcccaga tgccgtgcat gagacttcga tgctttcccc
     1681 aatacttgtc gtctgattct ccaggtttcc tgtgatcgtg ggtgccacac gctctaggac
     1741 tgtgagctgc ctgaccacgc aatgtctttt cttggtcttc ctgtcttgag caaggcagac
     1801 atagtctcct tggtcctgca aggatgcatt cttaagctcc atgatcaaaa tgtcatttgt
     1861 gctattagag aacatggtgg cattcaattt ccaaagagta tccaagttct tgcaaacagg
     1921 tgtgggcaac tctcccacat ggattggcag aggctgtggg ccaagcttgt accatgtgag
     1981 gttctcaaac gtagatctgt ctgcagtgca ccacaaagac acgctctcct gctcagtggg
     2041 ctgcatgtca ggttgcaaag taatttcagg acccctggtc acgtggaagg agatcaccct
     2101 ctctcctctc ccgactttgt tgaccgcttc acatttgtac aaagctgaca catttgccgc
     2161 ttggataaca agggtactta cagttttgtt ttttccttca attagagcaa attgattttt
     2221 attaacttca attttatttc ctccctggaa gtcctccaca cttctccatt cttcacaagg
     2281 gtatgggttt gtcactgaga cagcttggct gggctcgttg gcgcactctt cctccaactg
     2341 ccaataccag tggatgtgat gcgggggagg aatggcatag accgtacatg tcagcgtttg
     2401 agtggtgccg tactggtagg aatccacagg agagattaga gatttctcac caatctgggg
     2461 tgggacatac acaaccagag agaccacatg gctctgcttc tcctttgaaa tgggattggt
     2521 aaggatgaca gtgtaatttc ctgtgtctct ttcactcact tccataatcg tcagtacatg
     2581 ccccgcttta attgtgtgat tggactcaag gggtattcca tttttatacc attttatttc
     2641 tgggggtggg taaccaaggt acttcgcagg gattctgaca cgctccccca ccgtggcttc
     2701 caccagagat tccatgccac ttccaaaagc aacaaaaggt ttttcatgga ccctgacaaa
     2761 tgtgctgttc ttcttggtca tcagcccact ggatgctgca caggtgtaca atccttggtc
     2821 actccgggtt acaccatcta tagttaaggt gctcaaaaat ttcttcatct cactcccaga
     2881 ctgggttttt aggtctcggt ttacaagttt cttatgctga tgcttcgaag aagggtattc
     2941 ccagttgaag tcaatcccca catttagttc agttcttgct gtacaattta agacaagctt
     3001 ttctccaaca gatagttcaa ttccatgaga cggactcaga accacatcat aaatcctata
     3061 ccctacaacg acaactatgt acataataga ctggtaactt tcatcattaa tttttgcttc
     3121 acagaagacc atgccagcat agctgatcat gtagctggga atagtaaagc ccttcttgct
     3181 gtcccaggaa attctgttac catcaggaac aaatctcttt tctgggtatc ttgcacaaag
     3241 tgacacgttg agatttgaaa tggacccgag acatggaatc accacagttt tgtttttgtt
     3301 ctcagtaatg tacacgactc catgttggtc actaacagaa gcaataaatg gagatctgta
     3361 atcttgaaca tagacataaa tgaccgaggc caagtcagtt tcccggtaga agcacttgta
     3421 ggctccagtg tcatttccga tcacttttgg aattgtgagt gtcttacaga agaggccatc
     3481 gctgcactca gtcacctcca ccctttgctc actgccactc tgattattgg gccaaagcca
     3541 gtccaagtcc ctctgtcccc tgcaagtaat ttgaagagtt gtattagcct taattgtaag
     3601 tatgtctttt tgtatgctga gcctgggcag atcaagagaa acactaggca aacccacaga
     3661 ggcggcccgg gtctccacgc agagccacag ggcgacggcc agcagcacct tgctctgcat
     3721 ggtgaagggt actggatccg agctcggtac caagcttaac tagccagctt gggtctccct
     3781 atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag ttagccagag
     3841 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     3901 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     3961 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     4021 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     4081 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     4141 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     4201 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     4261 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     4321 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     4381 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc
     4441 ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc
     4501 ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa
     4561 cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     4621 agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg
     4681 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4801 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4861 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4921 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4981 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5041 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5101 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5161 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5221 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5281 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5341 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5401 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5461 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5521 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5581 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5641 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5701 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5761 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5821 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5881 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5941 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6001 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6061 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6121 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6181 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6241 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6301 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6361 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6421 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6481 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6541 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6601 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6661 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6721 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6781 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta
     6841 gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa accacaacta
     6901 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6961 ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg
     7021 cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag
     7081 aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt
     7141 tcgcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     7201 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     7261 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     7321 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     7381 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     7441 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     7501 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     7561 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     7621 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     7681 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     7741 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     7801 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     7861 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     7921 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     7981 ttgatcagat ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc
     8041 tccaaaaaag cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc
     8101 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8161 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     8221 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     8281 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     8341 taactgacac acattccaca gaattaattc gcgttaaatt tttgttaaat cagctcattt
     8401 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8461 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8521 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8581 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8641 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8701 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8761 cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga gccccagctg
     8821 gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct gctattgtct
     8881 tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga atgacaccta
     8941 ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg agtggcacct
     9001 tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca actagaaggc
     9061 acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg accggtacgc
     9121 gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg cgggccctct
     9181 agactcgagc ggccgccact gtgctggata tctgcagaat tgtcttgacc cttaacagga
     9241 ggagagctca gtgtggtccc cgagtcaggc tggagaatct gggctgtgct accggtttgc
     9301 actccaatct ctatcagctt taaaagttct gcttcctcac tggagtacac ggtggtgtct
     9361 gtgtcatcgg agtgatatcc ggactggtag ccgcttgtct ggtttgagcc ttcagatgcc
     9421 acagactccc tgcttttgct gggcaccatt ccaccaaaag atggagataa tttggttctg
     9481 tcttccaaag ttttcagctc ttctgaggca agaaccatac cactgtccgt ctggttgtca
     9541 tctgggatta cttttacttc tggttcttct aacgggatat c
//
