LOCUS       sequence-407444-       12786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, NUP98_NSD1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 12786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_NUP98_NSD1
FEATURES             Location/Qualifiers
     source          1..12786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(7547..8257,8258..8260,8261..8263))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7589..7610)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7938..7957
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8197..8218
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8260..8269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8345..8548)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8374..8394)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8549..8852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9109..9153)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9338..9571
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9931..10048
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10540..10665
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10712..10969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        10971..11350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11381..11485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11486..11554,11555..12346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11704..11723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12517..12786,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 acttctgttc tgattctgca cacttgtgac tggaaggagc ctggttaaga gctggaagtg
     1861 tattttgctc tggtttgggg tcttgccctc ttccaagaga ccagcaagtc tgtgccaatg
     1921 tctgtgtgct cccagcagac cagcaagact gtgccagtgt ctgtccagcc actatgggcc
     1981 agagccctgc ccgtgatgag gcttttccca gggcccaggg actttgctct gtggttacca
     2041 acttcttttc ttcagtgggg agggaggctg gggccttccc gagagaccta aaagattgcc
     2101 cactcttggc ggcaagtgca ggtagttgct ggcctccgac catctgcttc gcctgcttca
     2161 ccaggcccgg ggattggcta agaggccctg gggtctgctc agcccctgca gaagctcttc
     2221 ctgaggcctg agttgttggc tcatataaaa aggccttggc aggaggctga gaaagaagtc
     2281 tggcctgggt gagtgattta acagcttgcc aggggtcctc tgaaggggct gctgctttcc
     2341 cagatgtctg aagaggatct gacacacagc ctttctcaac agctctcggc atatgcccca
     2401 gacctttgct ggcaggccat gaacttgact ccaacactgg catcttctca tcagcctgtg
     2461 gtgtgacctg atgaggtgaa gctgcccgct ccttctggcg aggagttagg tctatgagat
     2521 ccatcaccaa agcagctcta ttttttgact gagtattctg gtccacaggc ctcagtgctt
     2581 tttctttagc tacaagggtc tggaccacag ctgatagtac tttctcagga ggtgggagtc
     2641 tctgggacaa agaggcatgg ggtttgtcag taggcctgtc tgaagtctgg ggtttgggac
     2701 tgctagtaat gagcagtctg tctggcggcg gaagtctcag gccagtggga actgaggttt
     2761 tctccagtga ctggggcctt gggctggcag cacctatgga tttatccagc cttgggtcag
     2821 ccgtccccag aggtctttcc agtggttggg acctgactga gggtgaggag cttgggctgg
     2881 tcactggaga gggtttgtcg cttagctggg gtcttggtcc tgccactgct ggtggcctgt
     2941 ccagtggcct ctggctggaa accaaggatt gggatttggt ccctgaccca gcgaggtctc
     3001 tgaccttatc taaaggctgg ggcctggagt cagttctctc aagaggtctt tcaggtagca
     3061 atggcctctg acaagtccct gccagagctt ttttggagag cgacagcatc tggttggtgt
     3121 cagcaggagg tttatctgac attttgggtg cctgagcagc cattcctgtt gattgctctg
     3181 ccaggtgagt gcttggccct ggaggcagcg gtactggggg aggcacatac tcacggatct
     3241 ccccaggttc cagaggattg ggcccacagg ggtcatgctc agtacaagac agacgcccat
     3301 ccagtttgga aatgaaaagc atcccttctc gatgctgctt acaaaaggag ctggggcaca
     3361 tctcacagaa ggaggctgct tccttcccgc agatgtcaca ctgatgccac ggacattccc
     3421 atttccctgc tggtcgcttg gtcagattga gacagtctgc gtggtaaact tttgggcagc
     3481 ctggtttctt gcaggagacg agctggccag catccccaca actaaaacac tcatcttctc
     3541 gctcctttgt gatttcaccc tgggtcctgc gctttccctg ttgcttcttc ttgaatttct
     3601 ttgacttttc ttccgtggca atgggttgat tctttggcct tacacccaag aagccactgc
     3661 agttcggggc tccacatttg caaacagtct ttccattccc aagacattct aggttgtagt
     3721 tgaaggtaag ttcagtgcct gctttaatgt cacttagtgc aaaaaggcct acacgggtat
     3781 ctccattcac agaccacttc tgtgtttcac agttgggctg gcagcaatga ttcatgaacc
     3841 gagcatagtt tcctttggga ccagcatcaa tgattcggtc tttgtctagg gtgagcatat
     3901 agaaattagt gatatcatgt tcttgagcat agcgaattcg agctctgcat tcttcttcat
     3961 ctataagctc acccacatac tcattcacaa attcaccctt tttaatatct gtttttgtcc
     4021 gtagacccca accccgctgt aatgtgcgga aaatttcaac ctctggatat tggcgcttgg
     4081 aaaagcactg gttttgacag cgccctccgg caggacacac tgtggggtgg cactcataga
     4141 gcagcatgcg gttgatgcat tcagagtcta tcccacaggg gttctcatca gtagctttac
     4201 agttgcaacg gggtatttca gataagtctg cagtgaagat ctgtaccctg ccaataggac
     4261 ggtttacctt tatatgttta taaggtggtg gcttcttgtc attctttcgg tcttcctgca
     4321 gctgtcttag ctctttttgg gcctttaatt cctcaaacct tgctgcagct tcctgaagag
     4381 cttttttata tgtcccatcc actcctttgc ccatcttatc cttgctgctc acgtcaccct
     4441 ccatgtaagg gaagactcgg gcctggtgag tccacaaata gtcattagat ccaaaaaaga
     4501 ggactgggaa ctctcccaca tcatgtctca tcttatcaat gttggaagga acagctcgag
     4561 gatggcagat ctcagctggc caccacctgt atcgtccaac ttttacccag acaatctccc
     4621 tgtagtgtgg ctttttgcct gctttacagt cattgcaata ccagtttcct tcagggatat
     4681 caatgttcag gcattcacga tgaaaagcag cagggcaaga atcacagcac agaaggctgc
     4741 ctccttctga gcacacaaag caccagctaa cattaacatg ctcatgattt cggcagcccc
     4801 gcctaggggt aaagtgatta gggcagatga tactattaga tgcaaggatc tttgacccag
     4861 cagccaggca aaagtcattg gcgtggtatg ccacaggaca gcggacacag cgcatcaacc
     4921 gacctttaga tgcagaaaca ttggctggat tagcagcatg acaggttata cagatgtgga
     4981 gggagcaccg gaagcccttg ttctgcataa cagtgggtgg gtacttctgg acacactctt
     5041 catggtaaaa ctttccacac aagggtagaa ggcacctttt aacatcttcc ccactctgct
     5101 tacatacaaa acaggtatgg attcctgtgc gacattcatt gcagataaat tttcctcttg
     5161 gcatctcagt caatccaagg cactccaggt ggaaagcccc acagcactga gcctcacata
     5221 acagcagctc acccaatttt tcacaattct gacagacatt ctccttgagt gcagctcctc
     5281 caccgcgttc accctgcatt tttttagagg caggcatccc gggatcgtgt tctacacctt
     5341 cctcaacagt ctccttgggg cttgtggccg tcctgtgagg cattagttct ccctctgagc
     5401 ctggaatctc ttttgacgag tcatcatttt tcactttttt acactgcatc ttggctgcag
     5461 catgcctctg tcgttttcgc ttccttggct tgtcaaatat cttggtagtg cctccaccaa
     5521 aatcttttga ataagatgtg gcacaagatt cagttatgcc attctccagg tgaccagctt
     5581 catagcactt tttagaaagg ccaaggtccc ccttcttggg cataaatcca gggtctaaat
     5641 cattggattc aagaagtttc ttagttggtt ttctttgacg tttactttca taatttcctg
     5701 gcgttttaac tagcaattcc tcagactcaa gggcaggccg tggagagact tccggagcca
     5761 caggcacaga caaggtcagc tgcggcaact cagcatgtcc acctccaagt tctgactgag
     5821 ccaaggggcc ctccaaaaac ggagcctccc tttcaagaac tggaggctct tctttggttg
     5881 aaatcaaaga attctcgtcc acctgcttgt tctgagcact agatcgacct cgggctagaa
     5941 ggctttcctc ttcacagcgg gaacttacct tgtgcacctg ctcctgtacc ttcttttgtt
     6001 ttttcttagg agcaaatatc tgatcatatt cttctgtata ttccaaaagc cacttgcttg
     6061 gcttcctaag gcgtttcttc tctgaccgca cagctactgg ggcctggggg gctccaaagc
     6121 ccaaagtggc tgtcgtagta ttaaatccag gggccccaaa ggctcctgta ccaagaggcc
     6181 ctccaatctt aggttggttg ttcccaaaca aagatgcctg tccagcacca agagctgttc
     6241 caaatcctgc accaagccca gttccaagag tcccaggtgc tggtttactt ccaaaaatac
     6301 tattcccact ggtatttgtg ccaaaaccaa agagcccgcc actggttgta ccaaatgaag
     6361 gtgcactggt tgtgctgctt ccaaatgtag taagcttgtt attgccaaac agggtcgaac
     6421 caacagcacc aaatccagta ttggtctgcc caaagagacc tgttcctgtt ccaaatgctg
     6481 tcccagtgct ggtgtttgta gctgtcccaa aaagacctcc aggctgtgag gcttgggtta
     6541 ctccaaataa tcccatggtg ttggtgcttg gctgtcctat ggtgctggta ttaccaaagg
     6601 aaaagccagt gttctgggtg gttgtagcct ggccaaatgg tttgctgaag aggctggtag
     6661 tctgctgatt ctgttggcca aagagaccac ctggatttgt tccaaatcca gttgtactag
     6721 ttccaaaggc agttttgttc tgaccatatg caaagcctga attagtggtg gaggagctga
     6781 agagtcctgt tgcgctggaa gtggctggag aagacccaaa caagccagtt gtggtacctg
     6841 ctcccacctg gttctgtggg cccttcctgt tagcctgata atcctctaaa cgaagttcct
     6901 ctagtgactt gctttcatat tctttcatag cagtaataca ctggtgcttg gtacttatgt
     6961 tagtgctaac tccagctttg accatagtat ctgtaccagt tggagggtta aatttaatag
     7021 tagtcccagt aggagcagct gtaaaactac ttggcccaaa gagggagcca gatgtgctgc
     7081 caaaaggatt agaggtggta tttgtggttc caaagagtcc tccactgcta gtactggttc
     7141 caaaattgcc aaagccagtt ggtttatttt gtgcaaaggc attgttttgg gatgagaaga
     7201 gactggtccc tgtgcttgca gttccaaaca aggtatttgc tgttcctgtt gacgtaccaa
     7261 acccaaagcc agtgcttgtg gaggtagctg gctggctaaa tgaactggtt ccaaacaatc
     7321 ctcctggttt agtctgtgaa tttccaaaga ggcctccagt attgttgcta gaaccaaatg
     7381 cagatgttcc aaatgcccct ccactagtag tgccaaagcc agtattctgt ccaaatgttg
     7441 aagttgtgcc aaagccacct gtgccacccc caaagggtgt tccaaatgat ttgttaaaca
     7501 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     7561 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     7621 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     7681 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     7741 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     7801 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     7861 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     7921 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     7981 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     8041 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     8101 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     8161 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     8221 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     8281 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     8341 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     8401 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     8461 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     8521 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     8581 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     8641 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     8701 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     8761 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     8821 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     8881 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     8941 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     9001 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     9061 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     9121 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     9181 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     9241 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     9301 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     9361 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     9421 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     9481 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     9541 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     9601 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     9661 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     9721 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     9781 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     9841 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     9901 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     9961 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
    10021 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
    10081 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
    10141 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
    10201 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
    10261 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
    10321 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
    10381 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
    10441 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
    10501 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
    10561 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
    10621 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
    10681 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
    10741 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
    10801 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
    10861 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
    10921 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
    10981 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
    11041 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
    11101 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
    11161 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
    11221 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
    11281 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
    11341 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    11401 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    11461 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    11521 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    11581 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    11641 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    11701 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    11761 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    11821 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    11881 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    11941 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    12001 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    12061 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    12121 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    12181 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    12241 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    12301 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    12361 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    12421 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    12481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    12541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    12601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    12661 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    12721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    12781 taccgg
//
