LOCUS       sequence-407585-       11389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific dsRed with AlutraF, eGFP in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795K
FEATURES             Location/Qualifiers
     source          1..11389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    6164..6549
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        6556..7038
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        7042..7633
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             join(7646..7648,7649..8362)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7646..7648,7649..8359)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7688..7709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7949..7968)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8296..8317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8472..8593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8509..8528)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8563..8582
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8621..8690
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   9160..9194
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9201..9219)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9240..9256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9240..9256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9253..9275)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9264..9280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9288..9294,9295..9312,9313..9318))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9333..9354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9471..9488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9642..10230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9722..9741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10401..11192,11193..11261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11024..11043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11262..11366)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca cctgtgggca cgatgatttt
     2761 ttatattagt agtatataat taatatcatt tgatttgtat atctacaatt ttccatgcca
     2821 ttttttgttg agtaatttta tcttatgcat ttctttcgta tttgtgtaca tacgtttaat
     2881 cattgcaaaa ttaatttatt tgttttatgc ttgattatgt gaataaaaat agcaacccaa
     2941 atggtgctga aaaagtgaat tttcaaaatg ttaaagctag cttttgtttg tttgctaata
     3001 ataaatcgaa gaaagtaatt ttaaataaag cactgtaggt agtcgagagt taactttaaa
     3061 aatgtcttca tgtattgatt tcgtatatca agcattatat ttatttacat tgttaaaaac
     3121 gtttgtgttg attgttgttc tcctcaggtg gcaaatgatg tttatttccc caacagtttg
     3181 ccattgtagt ttgagttcat tttgttgttg ttccagtatt ccctttaata gtttgttgat
     3241 tgtagtttgt tgattgaagt ttttaattgc tttgcttatt cgacaaactg taaaatatgt
     3301 tcagttggat attttagcta aaatatgggt tgaacccaat ataatccctg atttcatcag
     3361 ctctaaactc actcaagtca agactccagc agagacttta aataaacgct ccctaggtga
     3421 aactttaaga tatgaaaata agatgtatgt agttgtttta acagtttaca aaaccctcac
     3481 aaacaattta aattaaattt aattattgta agtaataaac aatttttagt gttaaaataa
     3541 ttattgtatg atcgctaaca ttcggaatta agtaacctgc caagcctgtg gtcatcagtt
     3601 gtcggcattt acctcataga gtaagagtag ggagcagact cttccggcaa agatggatgt
     3661 agaagttgaa aagcaagggg aaaaggacac tactatggca ccccttgctt tacgtcatct
     3721 ttttgcagat ttgatctcga attgaggaga gagtatcgac cacatagaaa aacgtttcca
     3781 ccacagtggg ttctacgtta ccgaaacgac ccggatttat atccggccaa ggactgtcac
     3841 tccagcagca atccccgtat gtaaatatgg ggaatgttaa tgctgctaca tcaacaacca
     3901 catagaaaat ttgcggttca cccaaggtac attgaacagg cagtagtagt tcatttgatt
     3961 tttatcattt atatggtctc caatttgtca aattttgttt aacgttaatt ttgttttcta
     4021 tacagtgtca atcttctttc tgcttataga ttttaacatt ctttggacca aagtcaaaaa
     4081 caaaatgcat tgctcttgtt gatgtgtgtc ttaggacaac ctcttcagtc cctgtgggag
     4141 agtggttttt tcaatgtcag ctacatcttt tcaagttcat aataagaaaa tagattgaat
     4201 atgaaattga aaatgatata ttgaaatgag aatgattaaa ttgaaattga gaaaattaag
     4261 ttgcaaatga gaaaatagaa ttcaaaatga gagaatataa aattgtatca tatcattggc
     4321 aagcttgagt gcttaagatt ctgataagcg ttgaagtcac cagctactaa tcggttttca
     4381 cctctgataa gagcaccaat accagggtga tttcctgcca ggcagctcgt gacagagggt
     4441 atatatatgt acattatata ttttgtacat attctgctat attttgatat tctaaagaat
     4501 tgtccctgtg gtcgatgtct tcatcgagtt atcttctcaa tcttgctctg gagtccgtta
     4561 aagttgaatc ttaatgattc tcggaagggg tgttacgttt acaaggtaaa gggcaaatgg
     4621 gattgtctac gttgcttatg cctgtgcgca ccgcttttgt tgttgtagtc tgatggtttc
     4681 ggacggattc gctacatgag atgagagaaa gagggttatc tgctcaggtg agccccggag
     4741 aaccgcgaag ggtacttgaa attgaaattt gtatatataa tataaatcct ttatagatat
     4801 aaacctacag gcacacatat ataatatgta acgaaaggta aattaattac caatcaatag
     4861 gccgcaaata tgagaatcca tttgaaaatg ccatcgaaac gattaaatat acatagtaat
     4921 aatttttatt tctccattat ctgtgctagc attcccattc ttatgtttgt gtgtgtttta
     4981 ctcatctatc tcgcaatgct ttccttctct gccaaactca agcaagtttt gctacttgga
     5041 aaattattaa ttattaaatt acagaaatgc cagcagtttt aaatatattg tgtgtgaaac
     5101 taaaacaatt gagatcaagc ttccgacatc caagtgggtc gaagtgggta tttagagagg
     5161 ccagggcacc ggctttccta cggtaccagc cacgatttac ccacaaaaat cgcatgccct
     5221 aattccaaag ccaaccccat tcttttaatc tagagcaggc ttctaagtag ctcgagtaaa
     5281 attgcactgc atatttctga tacagttaat tatatttctc aaaaaacgac ctattctagc
     5341 actgctcgtc ttgatataaa ttcttagtcg gagacttatc taatggtact cacttaagtt
     5401 tataagattc ttccattagt atgttgattg tagcctagtt ttgtccaatc tgacgtccac
     5461 atcactatca ttgatgaatg ttgattggtg tttgcaaacg tgcatttttg tatctttttc
     5521 acgcatataa attcaaccga gtatgttttc gtccttattg cttagtttta tatgaatttc
     5581 ataaaataaa atgtaggtta caattaccat caccagagac aggttgcggc ggcggttgga
     5641 tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg tgtcgctgta acagatgctg
     5701 ttcaactgtg tttaccagat cgttgcgggc tgtatttata ggcgcgataa gcgggacggg
     5761 cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct gatatggagg cgcgtcctgt
     5821 tccgataagg agttgcgtcc ggctgcggtt agcaacacag gaagctggcg tcctgtcacg
     5881 ataagacaac actcgtccgg tccgataatg tgattcgtac gtgacaggac gcgacccgat
     5941 aaggccggcc tacgtgactg ccgacacgta cttttttgca ctgcaaaaag gttcaatgtg
     6001 tggtagtgta tttggagcgt atacaacggt gtagactatt tatgtaaaat agtctacgaa
     6061 acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc gtgttttttt gcagtgcaaa
     6121 aaagttggtg gtggggaggc caccgagtat gggcgcggta cctcacgtaa taagtgtgcg
     6181 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     6241 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     6301 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     6361 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     6421 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     6481 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     6541 cgatcaattc tcgagcgcgt aaaacacaat caagtatgag tcataagctg atgtcatgtt
     6601 ttgcacacgg ctcataaccg aactggcttt acgagtagaa ttctacttgt aacgcacgat
     6661 cagtggatga tgtcatttgt ttttcaaatc gagatgatgt catgttttgc acacggctca
     6721 taaactcgct ttacgagtag aattctacgt gtaacgcacg atcgattgat gagtcatttg
     6781 ttttgcaata tgatatcata caatatgact catttgtttt tcaaaaccga acttgattta
     6841 cgggtagaat tctacttgta aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac
     6901 tgaactcgct ttacgagtag aattctacgt gtaaaacaca atcaagaaat gatgtcattt
     6961 gttataaaaa taaaagctga tgtcatgttt tgcacatggc tcataactaa actcgcttta
     7021 cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat
     7081 tgataaaatg aacggatacg ttgcccgaca ttatcattaa atccttggcg tagaatttgt
     7141 cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa
     7201 aggttttgcg cacagacaaa atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca
     7261 caaatcccaa cggcgcagtg tacttgttgt atgcaaataa atctcgataa aggcgcggcg
     7321 cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt caaggacggt gttatcgacc
     7381 tcagattaat gtttatcggc cgactgtttt cgtatccgct caccaaacgc gtttttgcat
     7441 taacattgta tgtcggcgga tgttctatat ctaatttgaa taaataaacg ataaccgcgt
     7501 tggttttaga gggcataata aaagaaatat tgttatcgtg ttcgccatta gggcagtata
     7561 aattgacgtt catgttggat attgtttcag ttgcaagttg acactggcgg cgacaagatc
     7621 gtgaacaacc aagtgacaat acaaaatgag caagggcgag gagctgttca ccggggtggt
     7681 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     7741 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     7801 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     7861 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     7921 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     7981 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     8041 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     8101 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     8161 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     8221 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     8281 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     8341 catggacgag ctgtacaagt aactagatca taatcagcca taccacattt gtagaggttt
     8401 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8461 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8521 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8581 tcaatgtatc ttaaagcttg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
     8641 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
     8701 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
     8761 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
     8821 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
     8881 cgacagatat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     8941 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     9001 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     9061 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     9121 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     9181 ctatctttct agggttaatc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     9241 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9301 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9361 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9421 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9481 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9541 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9601 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9661 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9721 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9781 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9841 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9901 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9961 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10021 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10081 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10141 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10201 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10261 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10321 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10381 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10441 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10501 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10561 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10621 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10681 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10741 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10801 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10861 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10921 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10981 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11041 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11101 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11161 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11221 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11281 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11341 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
