LOCUS       sequence-407611-        6262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a mutant Cocal virus G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_COCVmut_G
FEATURES             Location/Qualifiers
     source          1..6262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2967..2986)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3032..3087
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3086..3105)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3445..3461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3445..3461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3458..3480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3469..3485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3493..3499,3500..3517,3518..3523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3538..3559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3676..3693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3847..4435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3927..3946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4606..5397,5398..5466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5229..5248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5467..5571)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5597..6052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5734..5755)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5946..5965
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6201
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6193..6210
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6194..6210
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6220..6239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6220..6238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgaat tttctgctcc
     1441 ttacctttat agtgctccca ctctgtagtc acgcgaaatt tagtattgta ttcccccaat
     1501 ctcaaaaggg caattggaag aacgtaccaa gcagctacca ctattgcccc agtagttccg
     1561 accagaattg gcataatgac ctcttgggga tcactatgaa ggtgaagatg ccagcgacgc
     1621 acaaggctat ccaagccgac ggctggatgt gccatgcagc gaaatggatt acgacatgcg
     1681 attttcgctg gtacgggcca aagtacataa cacacagcat acatagtata caacctactt
     1741 ccgagcaatg caaggagagt attaagcaaa ctaagcaggg gacctggatg agcccgggat
     1801 ttccgccaca gaactgcggt tatgccactg tgacggatag tgtagctgtc gttgtccaag
     1861 caaccccgca tcacgtcctc gtcgatgagt acacgggtga atggattgat tctcaattcc
     1921 ctaacggaaa atgtgaaaca gaggaatgtg agacagttca taattcaacg gtgtggtatt
     1981 cagactacaa agttacaggc ttgtgtgatg cgacgttggt cgacacagaa atcacgttct
     2041 ttagtgaaga tggcaaaaag gaatccatcg gaaaaccaaa cacaggttac cgcagcaacg
     2101 catttgctta tgaaaaaggg gacaaggtct gtaaaatgaa ttattgtaaa cacgcgggtg
     2161 tgagactccc atccggcgtc tggtttgaat ttgtggacca ggatgtctat gcggcggcta
     2221 agcttcctga gtgcccagtc ggtgctacta tcagtgcgcc cacacagaca tcagttgatg
     2281 tcagcctcat actggatgtc gaacgtattc tggattactc cctttgccaa gagacatgga
     2341 gtaagatcag gtccaagcag cccgtaagcc cggtagacct gtcctatctc gcaccgaaga
     2401 accctggtac tggacctgct tttacaatca taaatggaac ccttaaatat ttcgaaactc
     2461 gctacatacg cattgacata gacaacccga taatctccaa aatggtcgga aaaataagtg
     2521 gctcacaaac ggaggccgaa ctttggacag aatggtttcc ttatgaaggg gtggagatcg
     2581 gacccaacgg aattttgaag acaccaacag gatacaagtt tccactgttt atgattggac
     2641 atggcatgtt ggattctgac cttcacaaaa cgtcccaagc agaagtattc gagcacccac
     2701 atctcgccga agcgccaaag cagcttcctg aagaggaaac tcttttcttt ggtgacacgg
     2761 ggattagtaa gaatcccgta gaactgattg aggggtggtt tagttcctgg aagtcaacag
     2821 tcgtaacctt ttttttcgca ataggtgtgt ttattttgct ctacgtggtc gccaggatag
     2881 ttatcgccgt ccggtaccgc tatcaagggt ctaataataa gcggatatac aacgacatcg
     2941 agatgagtcg atttcgcaaa tgaagatctt tttccctctg ccaaaaatta tggggacatc
     3001 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3061 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3121 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     3181 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     3241 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     3301 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     3361 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     3421 ggatcggccg caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     3481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4261 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4741 accgcgagac ccacgctcac cggcaccaga tttatcagca ataaaccagc cagccggaag
     4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5581 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     6061 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6121 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     6181 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     6241 gaattggagc tccaccgcgg tg
//
