LOCUS       sequence-407849-       12817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1046
FEATURES             Location/Qualifiers
     source          1..12817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7760..7771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    8674..9262
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8727..8747)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9145..9156)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9334..9567
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9645..9766
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9682..9701)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9736..9755
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9806..9941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9961..9980)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9962..9980)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9990..10007)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9990..10006)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9999..10021)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10148..10603
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10235..10254)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10445..10466
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10629..10733
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10734..10802,10803..11594)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10952..10971)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11765..12353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12254..12273
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12507..12524
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12641..12662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12677..12682,12683..12700,12701..12707)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12715..12731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12720..12742
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12739..12755
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12739..12755
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12774..12794
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12776..12794
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg ccgctcctgg gccgcaagcc
     4141 cttcccgctg gtgaagccgt tgcccggaga ggagccgctc ttcaccatcc cgcacactca
     4201 ggaggccttc cgcacccggg aagagtatga agcccgcttg gaaaggtaca gtgagcgcat
     4261 ttggacgtgc aagagtactg gaagcagtca gctaacacac aaggaagcct gggaggaaga
     4321 acaggaagtt gctgagcttt tgaaggagga gtttcctgcc tggtatgaga agcttgttct
     4381 ggaaatggtt caccataaca cagcctcctt agagaagtta gtagatactg cttggttgga
     4441 gatcatgacc aaatatgctg tgggagaaga gtgtgacttc gaggttggga aggagaaaat
     4501 gctcaaggtg aagattgtga agattcatcc tttggagaaa gtggatgaag aggccactga
     4561 gaagaaatct gatggtgcct gtgattctcc atcaagtgac aaagagaact ccagtcagat
     4621 tgctcaggac catcagaaga aggagacagt tgtgaaagag gatgaaggaa ggagagagag
     4681 tattaatgac agagcacgta gatcgccacg aaaacttcct acttcattaa aaaaaggaga
     4741 aaggaaatgg gctcctccaa aatttctgcc tcacaaatat gatgtgaaac tacaaaatga
     4801 agataagatc atcagtaacg tgccagcaga cagcttgatt cgtacagagc gcccaccaaa
     4861 taaggagata gttcgatact ttatacggca taatgcatta cgagctggta ctggtgaaaa
     4921 tgcaccttgg gtcgtagaag atgaattggt gaagaaatac tctctgccca gcaagttcag
     4981 tgacttttta cttgatccat acaagtatat gactctcaac ccttctacta agaggaagaa
     5041 tactggatcc ccagacagga agccctcaaa gaaatccaag acagacaact cttctcttag
     5101 ttcaccacta aatcctaagt tatggtgtca cgtacacttg aagaagtcat tgagtggctc
     5161 gccactcaaa gtgaagaact caaagaattc caaatctcct gaagaacatc tagaagaaat
     5221 gatgaagatg atgtcgccca ataagctgca cactaacttt cacattccta aaaaaggccc
     5281 acctgccaag aaaccaggga agcacagtga caagcctttg aaggcaaagg gcagaagcaa
     5341 aggcatcctg aatggacaga aatccacagg gaattccaaa tctcccaaaa aaggactgaa
     5401 gactcctaaa accaaaatga agcagatgac tttgttggat atggccaaag gcacgcagaa
     5461 gatgacacga gccccacgga attctggggg tacacctagg acctctagta aacctcataa
     5521 acatctgcct cctgcagccc tacacctcat tgcatactac aaagaaaaca aagacaggga
     5581 ggacaagagg agcgccctgt cctgtgttat ctccaaaaca gctcgtcttc tctctagtga
     5641 agatagagct cgtctcccag aagaattgcg aagtcttgtt caaaaacgct atgaacttct
     5701 agagcacaaa aagaggtggg cttctatgtc tgaagaacaa cggaaagaat atttgaaaaa
     5761 gaaacgggag gagctgaaaa agaagttgaa ggaaaaagcc aaagaacgaa gagagaaaga
     5821 aatgcttgag agattagaaa aacagaagcg gtatgaggac caagagttaa ctggcaaaaa
     5881 ccttccagca ttcagattgg tggatacccc tgaagggctg cccaacacgc tgtttgggga
     5941 tgtggccatg gtggtggaat tcttgagctg ttattctggg ctacttttac cagatgctca
     6001 gtatcctatt actgctgtgt cccttatgga agccttgagt gcagataagg gtggcttttt
     6061 ataccttaac agggtgttgg tcatcctctt acagaccctc ctacaagatg agatagcaga
     6121 agactatggt gaattgggaa tgaagctgtc ggaaatcccc ttgactctgc attctgtttc
     6181 agagctggtg cggctctgct tgcgcagatc tgatgttcag gaggaaagcg agggctcaga
     6241 cacagatgac aataaagatt cagctgcatt tgaggataat gaggtacaag atgagttcct
     6301 agaaaagctg gagacctctg aattttttga gctgacgtca gaggagaagc tacagatctt
     6361 gacagcactg tgccaccgga tcctcatgac atactcagtg caagaccaca tggagaccag
     6421 acagcagatg tctgcagagt tgtggaagga acggcttgct gtgttgaagg aagaaaatga
     6481 taagaagaga gcagagaaac agaaacggaa agaaatggaa gccaaaaata aagaaaatgg
     6541 aaaagttgag aatgggttag gcaaaactga taggaaaaaa gaaattgtga agtttgagcc
     6601 ccaagtagat acagaagctg aagacatgat tagtgctgtg aagagcagaa ggttgcttgc
     6661 cattcaagct aagaaggaac gggaaatcca ggaaagagaa atgaaagtga aactggaacg
     6721 ccaagctgaa gaagaacgaa tacggaagca caaagcagct gctgagaaag ctttccagga
     6781 agggattgcc aaggccaaac tagtcatgcg caggactcct attggcacag atcgaaacca
     6841 taatagatac tggctcttct cagatgaagt tccaggatta ttcattgaaa aaggctgggt
     6901 acatgacagc attgactacc gattcaacca tcactgcaaa gaccacacag tctctggtga
     6961 tgaggattac tgtcctcgca gtaagaaagc aaacttaggt aaaaatgcaa gcatgaacac
     7021 acaacatgga acagcaacag aagttgctgt agagacaacc acacccaaac aaggacagaa
     7081 cctatggttt ttatgtgata gtcaaaagga gctggatgag ttgctaaact gtcttcaccc
     7141 tcagggaata agagaaagtc aacttaaaga gagactagag aagaggtacc aggacattat
     7201 tcactctatt catctagcac ggaagccaaa tttgggtcta aaatcttgtg atggcaacca
     7261 ggagctttta aacttccttc gtagtgatct cattgaagtt gcaacaaggt tacaaaaagg
     7321 aggacttgga tatgtggaag aaacatcaga atttgaagcc cgggtcattt cattagagaa
     7381 attgaaggat tttggtgagt gtgtgattgc ccttcaggcc agtgtcataa agaaatttct
     7441 ccaaggcttc atggctccca agcaaaagag aagaaaactc caaagtgaag attcagcaaa
     7501 aactgaggaa gtggatgaag agaagaaaat ggtagaggaa gcaaaggttg catctgcact
     7561 ggagaaatgg aagacagcaa tccgggaagc tcagactttc tccaggatgc acgtgctgct
     7621 tgggatgctt gatgcctgta tcaagtggga tatgtccgca gaaaatgcta ggtgcaaagt
     7681 ttgtcgaaag aaaggtgagg atgacaaatt gatcttgtgt gatgagtgta ataaagcctt
     7741 ccacctgttt tgtctgaggc cggccctcta tgaagtacca gatggtgagt ggcagtgccc
     7801 agcttgccag cccgctactg ccaggcgcaa ctcccgtggc aggaactata ctgaagagtc
     7861 tgcttctgag gacagtgaag atgatgagag tgatgaagag gaggaggagg aagaagagga
     7921 ggaggaggaa gaagattatg aggtggctgg tttgcgattg agacctcgaa agaccatccg
     7981 gggcaagcac agcgtcatcc cccctgcagc aaggtcaggc cggcgcccgg gtaagaagcc
     8041 acactctacc aggaggtctc agcccaaggc accacctgtg gatgatgctg aggtggatga
     8101 gctggtgctt cagaccaagc ggagctcccg gaggcaaagc ctggagctgc agaagtgtga
     8161 agagatcctc cacaagatcg tgaagtaccg cttcagctgg cccttcaggg agcctgtgac
     8221 cagagatgag gccgaggact actatgatgt gatcacgcac cccatggact ttcagacagt
     8281 gcagaacaaa tgttcctgtg ggagctaccg ctctgtgcag gagtttctta ctgacatgaa
     8341 gcaagtgttt accaatgctg aggtttacaa ctgccgtggc agccatgtgc taagctgcat
     8401 ggtgaagaca gaacagtgtc tagtggctct gttgcataaa caccttcctg gccacccata
     8461 tgtccgcagg aagcgcaaga agtttcctga taggcttgct gaagatgaag gggacagtga
     8521 gccagaggcc gttggacagt ccaggggacg aagacagaag aagtagtaat gaactagtcc
     8581 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaagcta
     8641 acaataggaa atggcattac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     8701 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8761 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     8821 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     8881 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     8941 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9001 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9061 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9121 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9181 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9241 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     9301 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9361 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9421 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9481 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9541 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     9601 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     9661 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9721 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     9781 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     9961 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    10021 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    10081 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    10141 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10201 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10261 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10321 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10381 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10441 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10501 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10561 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    10621 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10681 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10741 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10801 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    10861 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    10921 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    10981 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11041 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11101 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11161 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11221 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    11281 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11341 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11401 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11461 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11521 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    11581 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    11641 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    11701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    11821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    11881 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    11941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    12481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    12541 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    12601 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    12661 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    12721 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    12781 accctcacta aagggaacaa aagctggagc tgcaagc
//
