LOCUS       sequence-407939-       14015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tudor expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-PH-mCherry-Tudor isoform A (HK130)
FEATURES             Location/Qualifiers
     source          1..14015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2984..3691
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3129..3147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3397..3416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             11321..11362
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             11372..11389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11412..11429)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11462..11596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11486..11505)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11540..11559
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(12197..12785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12277..12296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12956..13747,13748..13816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13579..13598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13817..13921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13989..14007
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggcccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatgcac ggtctccagg atgatccaga tctgcaggct ctgctgaaag gttctcagct
     2641 cctcaaagtc aaaagcagct cctggcgtcg tgaacgcttc tacaaactgc aggaggactg
     2701 caaaaccatc tggcaggaat cccgtaaagt aatgcgctcc ccagagtctc agctcttcag
     2761 cattgaggac attcaggagg tacgtatggg tcaccgtacc gaaggtctgg agaaattcgc
     2821 tcgcgatatc ccagaagatc gctgcttctc tatcgtcttc aaagaccagc gcaacaccct
     2881 ggatctgatt gctccatctc cagcagatgc tcagcactgg gttcagggtc tccgcaaaat
     2941 cattcaccac tccggttcta tggaccagcg tcagaaaggt accatggtga gcaagggcga
     3001 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3481 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3541 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3601 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3661 ctccaccggc ggcatggacg agctgtacaa gccaagcttg aattctgcaa cgtgcatgaa
     3721 tggacaggcg cgcattgctc ttccctccaa agtggacctg tacatcactc atgtggatca
     3781 cgtgggtccg tatctgaagg tctacggcca cgttaaccga gacgccgcct ccctgatcag
     3841 cgaacgaatc aggaatctgt tgcccacatg ctttgccatt gagcccagtt ggtccgtgga
     3901 gcgccaacag gctctcctca tacccggcac gttttgcata ttcaagaata tcaatggccc
     3961 ggctcctggt gatgtggagt acaggcggat acgtgtggtc agtgcagacc tggagggtca
     4021 gtcgatgcgg gcggagatcg atttcgtgga ctttggctat aatcgtactg tagatagtca
     4081 tgatttaatg ttccccaagc agcccaagct gcttcagaac attcccctgc actgcttcca
     4141 gtacattgtg ctgggaatct gctccgagtg ggatcagacc gatctggctg aggttcggcg
     4201 gctggttgtc aaccagatcg ttaaaatcac tgtggaacct acacaaatct gtgaccaaaa
     4261 gtttgccagt ctgcgctgga aggattttga gctcaacgag ttcttagtgc aacaaaaaca
     4321 aatcggagtt tcggtcgaca agcaactcat gatggaccac tgcaagaagc tgtggaagga
     4381 taatccgcag tcgccggtca ccgaatacaa caacaatagt atacacaact caaagacgcc
     4441 catggagatt gcccgtgagc aactagcggt gcgacaatcc ttagctgctc gcttggatgc
     4501 acagcgatcg gtccaagtga caccgtcaag gccgctaaac gccgatgccc ctgactatac
     4561 gcctaaacat ctgccgctgg tcaatgtgac aaatgtacag gtacaaatgc caggcctaaa
     4621 caatactcag aaaccggtat ttgtatcgac aaatccttat aatcgtgcca actatcagcc
     4681 cgctgtgccg gctgctcaac catatgtgcc caaggcgaac ccccgatcgc aatacaccta
     4741 ttacaatgtt cgcatgaaca aaccgattaa cgccatgccg ccgccggccg gtccacatgt
     4801 gcccattcag cactttaatc aacaggctaa caacgtgtca ctgagctatg ttcctgcacg
     4861 gttcactcca cctccaacgc catcgattgc gcaacatcaa atccccattc ctgccttcag
     4921 aaccaccagc ttaacggtgg gcctcaccta cgacgtggtt ataagctatg tggagaacgg
     4981 tccgtaccta ttttgggtgc acctgaaaag cagcgatcat gatctgagca ccatgatggg
     5041 tcaaattgaa cggacgaagc tcaaggcact cgcgcaggct cctgagcttg gaactgcctg
     5101 tgtggcccgt ttctcagaag atggtcatct gtatcgcgct atggtgtgcg ctgtttacgc
     5161 ccaacgttac cgcgttgttt acgtggacta cggaaactct gagttgttgt ccgcaagtga
     5221 tctgtttcaa atacccccag aactgttgga aattaaaccg tttgctttcc gatttgcttt
     5281 ggccggaacc aaggaaattg agcccattga cgacagcatg aagcggattt tcaagaaatc
     5341 agccatttat cgtaactttg aactgactgt ccaggcgcca gagagtgtgg gctccatgca
     5401 aacctgccat ctcaaccaga atggcaccaa tatgctggag cttcttaggc agctaaagaa
     5461 ctcacgtcag tcctacaaaa aggcggagca acttgaaaac gacgacgccg tggagattcg
     5521 ttttattgat tcgcccagca acttctatgt gcagaaagtg gccaacatag gaaagttcga
     5581 gcagctcatg gacgagatgt tctcctacta caatgcaaac cagagagttc ccgatcagtt
     5641 gatcttgggc gcaccctgca ttgttaaatg cgatcaggaa tggtaccgtg cagagattct
     5701 gcgcgttgat gactctgtga ttgtgcggca tgtagatttt ggttatgaac agaatgtgaa
     5761 gcggcatttg attggccata ttgctgagaa gcatctggaa atgcctcgcc aggcaattaa
     5821 gtgctgcttg aaaggattcg agaacagtga acttagcgag gacaagatta ccgatcagtt
     5881 tgaaatgtta gctgaggaat ccaatatccg aaggcgcaca tttagtgtgc gtatctttcg
     5941 cattgaaccc gatggtctga acgtagtaaa cctgctggcc aagaacctga atgtgatgaa
     6001 gaagctctac aagctttcta tgccttttga acaatatctg tccctggaaa agggtcagct
     6061 taatgcaaac aacacccgtg ccgagtctgt gatctcttct gagttgaata agagccatat
     6121 tttgaactct acaagcattg gcgagaccga aaatcgtttg caagaacagg aaaaacagca
     6181 gcaacagaaa aaggacgatg ttaggcagca gcaactagca gttgaaattc cgcaagcagt
     6241 aaagtccgtt agcggaagca aaaactcgac agactgggac aaacgtagct ccacctcggc
     6301 aggctcaaag gacagtaagc gtcagcagca gcaacaaata cagcgagtcg accgccattt
     6361 agacttcagc tgtgagacac agagcaccgg tagttacagc agtggcatga gttcgccgcg
     6421 caagggtaac cgacaacaga acggtcgcac accgatccaa tcaccacgcc ataatgaaaa
     6481 gcaggaagcg aaaaaaaatg cgcgctttag caacagcgag tcaccccgca ggagtcgaga
     6541 tggccagcag gggaaccaac gctcccaaaa tgcgccacag ggctatgctc aaaagccaca
     6601 gcgtcaaaaa tccacgttag acggaaatat cagctcaaag cgttccagcg gagtggggag
     6661 tgatattgcc tcatccagct ccgaatcggt ggctgccgcc aagccggaga aatacgtttc
     6721 cttggataaa ccgtatgcgc tgcaggagat gaaaacacct agcaaggagg cagctagttt
     6781 gtcttggtgg ctctcgccat tccagttcta catcgtgccc aagtctgtct ctgcaaagta
     6841 cgataatata atgcgtgata tgcgagagtt ctaccgccag aagcaacacc agccacttca
     6901 attaaaggtt ggatccaccg tagttgtgcg ccagcgaaag gacaatgcca ttctgcgggc
     6961 tactgtgacc gcatgcaatc acatgatgcg aaagtatcgc gttttttgcg tggacacagg
     7021 cagcttaata acagtcacct cggaggatat ttggcaattg gagcagcgct ttgcagatcc
     7081 gccctgcatg gcacatcgct gcagctttca cagcgtcgtt accaactatg atccattgta
     7141 cattgtggat cgcatggaaa cgttcgtgcc agttaatgcc aaagtcgagt gcgagttcgt
     7201 gtccaaagag aagagcaacc aaggctcgaa taccagcagc acttgctcct acacagttaa
     7261 tatttttgta aacggagcat ccctgcgaga tatgctcgtc aaagcggaat tccttaccga
     7321 agtggcgcct gagattcgcg ttaacctttt agctggtcag cagattagag gaaaattcac
     7381 ttctattcgt gacatgacaa gctttaaggt tcagtttgat tatggtaaca atgtgaactt
     7441 cctttgcacc tatgacgatg ctaaatttgt taagtccaat ccgaatttgg ccaggcgatt
     7501 taaggagttt tacgagggta aatcgtttgc cttgaatgtc aagaatgtct gcgaaaacaa
     7561 tatcgttcat ctgagacctg taatgccgtt gttcatggag gatcgcaggt cattcatctg
     7621 cccctatccc gtggtgctga gctcattcca agcactcgtt gtgtacaccg ctaaacctta
     7681 ccgcgtgtat gttcaaccgc aggccatagt accatctatg cagaccctat tggataatat
     7741 gtatgaacac tacaaggcca agggcgactc tttgaaaaaa tttgatgtgg gacagatctg
     7801 tgctgtacga agctccgatg gcaattggta cagagccaga atctcaggaa aggattcgaa
     7861 tgcagcgtgc ttcgaggtgt tttacatcga ttacggcaac acggaggaaa taaagcgtga
     7921 cgatatcaag gcattggatg caaagtttta tgagcacgca agcggctttg cagtggagat
     7981 taatttgccg attggccgac ccagcaacga tacaaagcta aaggcgcgta tatccgagat
     8041 tcttgaggag aaggttgtca ctatcaaatc gattgaggtg cggcgcagtc acctaattgc
     8101 cgatgtcatt ttggaaaaca atcagagtgt gatagacctg ctaaaggctg agaagcttgt
     8161 acccggcaaa gatttggatt acatgcgcaa gcaaatggag aaaggaaagt ctcgcactta
     8221 cgagtacatt gaaacagtag acctaacttt ggacgaagag gaggataagg gtcgcaagga
     8281 aactgtcagc aagtcgggtt cggcaaatgc ctccccaaag aaaaaacaac ataatgacaa
     8341 ggatcgcgag cccaaaaaga gtaagccagc agagcctgct cgtactgttg ctccacaacc
     8401 tgttgcattg aaaactcctt caccagttcc ggcggagcca gcaccagtgc caaaaccagc
     8461 cactccagtt ccagaggttg ttgaagtacc agaaattaat ccaactgtca gagaagctgc
     8521 tgccgagtca aagcaagctc ctgcacaaga ggatccttac aaagatttgg actgcgttgt
     8581 actgagccat tgcgacaatc ctgcacagtt ctatgtccac ccgatcgatc agctatcgaa
     8641 actaaatcag ctgcatgaga accttcagat tgtgtctccc tctttgcccc agttgatgaa
     8701 cgtggtaaac ggtgcagact gtgtatcgat gtattcggtg gacaaatgct ggtatcgcgc
     8761 caaaatcatt gatgccgagc taatggtact acttttcata gactatggca acacagattg
     8821 cgtttcggat gccactgaca tcaaagaaag catgtggtcg catatcgaac ccttctgctt
     8881 gccatgcgca ctcccgattc gacccaaggg tactgcggat tgggtggatg cagcgaatgg
     8941 catctttaac gaatcgtact cgaaggtccc gcgtctggag taccttaccc aaggagatca
     9001 ctacacgacc agctatgtta acatgtatat tgatggcgag gatgtggcta agaaattaat
     9061 tgccgatggt tttgctaggc cgttagagta tttggccagc ggatgcagtt gctacatttc
     9121 gcacgtgaat ggaatttgtg atttctttat tcaacttgaa cgtgattcaa aggcgctaga
     9181 actaattgag ttgtatttgc gcaagaagga cacattaaag cccctcgaag gatttgaaaa
     9241 gggtttgatt gtggctgctc tatttgagga cgatgagctg tggtaccgag cccagttgca
     9301 gaaggagttg cccgattccc gatacgaagt actctttatc gactacggta acacctccac
     9361 cacatcgaag tgtctaatgc tgtccgaaga gattgctagc ctacccagct tatccaagaa
     9421 atgttcgctg cagttgccag atgcctacat ctcatggtca ccagaagcgg aggccaaatt
     9481 cgccgaactg acaggcgagg gtgagctagt gtttaccaca cagcttctga agccaggaca
     9541 ggatcatgtc accattgatc ttctcctgga tggagagaac atcatcgacc gcctgttacc
     9601 gttgtgccag cgaaaggaac ccaaggaagc cagtaaggag tccttagctg ttaccactaa
     9661 agccatcatt acgcacgtgg agaatacatc ccgtatatat ctacagttta gcgagaagga
     9721 ttcattaatg gatattatat gcgagaagct aaatggaagc aagctgcagc ctaagacgga
     9781 aaaggctgca gtagatgata tgtgcgtagt tcaattcgcc gacgacttgg agttctatcg
     9841 ctcacgtatt ctggaagtgc tcgaagatga ccaatacaaa gtcattttga ttgattacgg
     9901 caatacgact gtggttgata agctttatga gttacctcaa gagttcacgc tcattaaacc
     9961 tgtggccgaa atatgcagca tggaacccag cgccatattt gagaaaaata aagctctaac
    10021 cctgacaaca tttgacgcac tgctggacag ctgcaagggt gttgtggcgg tggaatttgt
    10081 aaacaagtct gccagtcctc ctgtggtcag attgactact aaagataaga ggagcctgaa
    10141 gatctatgaa caccttcaga agttggttca agccgagctg aaactaatcc aaaagcgaaa
    10201 cgagaactct gaatgcatta tctcatatgg aaactcaccc aagagttttt atgtgcaaat
    10261 gaagcacaat tcagcggatt tggacttgat tgtgaagaca ctgcagtcct taaaaaagga
    10321 gaaactgaag aaattaatcg atcctactac aaattcaaat ggtgtttgct actcccaaga
    10381 ggatgcctgt tactatcgct gtagtatcaa gtcggtattg gatcctagcc agggatttga
    10441 agtctttttg ctagattatg gaaacaccct ggtcgttcca gaggtctggc agctacccca
    10501 agagatagag ccgattccat cgttggctct acactgtcag ctgagtaaga ttccaatgga
    10561 tgtgtctgac gaaaagctag aagaagcctt tgctgctttg ctagagcagc acttcggaga
    10621 actatacgaa ataactactc agccgaacga ggatgagaca aaacctctga ttgctgaact
    10681 ccgaatcaac tacaaggatt tcgtccagga gctggtcagt acggtgaccg gcgtacagaa
    10741 accgctcgaa gcggagctgc acaactgtgt tgtggtgcag ttcgatggcc ccatgtcctt
    10801 ctacgtccag atggagagcg atgtgccagc actagagcaa atgactgata aactgctcga
    10861 tgcagaacag gacctacccg cctttagcga cctcaaagag ggagctttgt gtgtggctca
    10921 gttcccggag gacgaggtct tctatcgcgc ccaaattcgc aaggttttgg atgacggaaa
    10981 gtgcgaggtg cattttattg actttggcaa caatgccgtg acgcagcagt tccgccagct
    11041 gcctgaggag ttggccaagc cggcgcgata cagcaggcat tgcgagctgg atgcgtcgac
    11101 catatccaag tgcgacgcgg ccctgctcca gtcgttcatc gacactcgct tctctgaaac
    11161 attccaggta gagattctgg ccaccaaggg aactggcacc catgtcgtac gactcttcta
    11221 ccagagcaag aacatcagcg agaagcttca ggaatgtcag tgagcatgag atatccagca
    11281 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
    11341 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
    11401 ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga
    11461 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
    11521 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
    11581 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
    11641 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
    11701 tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
    11761 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
    11821 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
    11881 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
    11941 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
    12001 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
    12061 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
    12121 tagatgacga ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg
    12181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat
    12241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    12301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    12361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    12421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    12481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    12541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    12601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    12661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    12721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    12781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    12841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    12901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    12961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    13021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    13081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    13141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    13201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    13261 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    13321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    13381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    13441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    13501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    13561 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    13621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    13681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    13741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    13801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    13861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    13921 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    13981 cctataaaaa taggcgtatc acgaggccct ttcgt
//
