LOCUS       sequence-408075-        9162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, GSN_NTRK1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_GSN_NTRK1
FEATURES             Location/Qualifiers
     source          1..9162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3923..4633,4634..4636,4637..4639))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3965..3986)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4314..4333
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4573..4594
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4636..4645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4721..4924)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4750..4770)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4925..5228
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5485..5529)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5714..5947
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6307..6424
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6916..7041
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7088..7345
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7347..7726
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7757..7861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7862..7930,7931..8722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8080..8099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8893..9162,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatc
     1801 ctaatacatc caggtatacg ggcggggcct gggcaagtgc ttgcaggcgt gcgtggacgt
     1861 ccttgatcga atgacgttgc tgtggttcac gctgccaaca gccacgcatg atcgcatata
     1921 cttcaggagg acacgcacgg ggacgctcca gttcgcggcc ctgagtgatg caatcaatag
     1981 cctccgtatt agacaactgg taccagggtt gtttcccata agtaaaaatc tcccacagca
     2041 caactccgaa agaccataca tccgactcag tcgtaaattt gcgatacaag atcgattcag
     2101 ggggcatcca acggatgggc agcatcgtgc gtccccccac gcgataatag tccgtgctgt
     2161 agatgtcacg tgacataccg aaatcgccga ttttaacaac cagaccttgt cctaccagac
     2221 aattgcgtgt tgccagatcg cggtggacga aatgcagtcc tgccaaatac accattccag
     2281 cggctacctg cgatgcgaca gctaaaagct gtcctaatcc caaaggaccg ggcgcgacat
     2341 cttcgccacc ggccagcagt ttggcgtccg gcccatggct acgcagaaag cgattcaagt
     2401 caccgtgacg catgtattca aagaccatca gcaacgggcg cccctcagta cagacgccaa
     2461 aaaagcgcac gatatgttgg tgttgtaaca tagttaacag ttccgcttca cgctgaaaat
     2521 cttggcgtgc agattcagaa gcctccttca aggccttcac agctactaac atcttatcct
     2581 gctcgggcag caagttgtga cattctgcca gaaaaacctt accaaaggcc ccctctccta
     2641 actcccactt caatacgatg tcgcggcgct taatatggtg gacgcaagcg tctgagaagt
     2701 actgagggtt ttcaataata tggccttgca ggccgctccc ttttccttct gtggggctca
     2761 acgaggagcc tcccaacgtc atgaaatgca gtgacatagc taaaccatct tctggggcaa
     2821 gaaccgcggg acggttgatt ccgaatttat tgcggcgacc acatttgttc aaaaccagca
     2881 ataaagttga aagaaataaa caggcgaaca cagcaagacc aacggcaact gaaaccccga
     2941 agggggtttc gtctttcttt tctacggggt caccgctagt agagttagta tctttccaaa
     3001 cgaaaatctt cccgtctttc ccgtggtcaa gaataaagca gtcttcagat ttaagggcgc
     3061 cttgagcgaa cgggttctca tccgcaacaa gcgatactga catagtacct gctccatttg
     3121 agactttgta caatttggca agtttacgat ttgctgcatc ctccttggca gtatcctcag
     3181 tccctgcggg cagtgcaggc ttaggtccca gaacttgcaa cattgcttcc ggctccgttc
     3241 cttcttcaga cacatgtacg cgagcacgac cactgcgctc gttgtcgcgg ataccttttg
     3301 acacctgtgt tgccttaaga cgttcataac gattcgagtt cgacccacac cactgatgga
     3361 tattattacc caagtccagg ataaaacaat ccccgttatt gaaagattcc caactgaccg
     3421 gtacctcagt cgcgcgaaca acgcgacgcc ccttgacctg aaataaacgc tggaccacaa
     3481 cttcgttagg cacgacatgc ttgaatcctg atgcgacccc gcccttttta tattttaagc
     3541 ctgacttgaa atagcccaaa aacgttgccg attcaaagcc ctgaacctcg cgatgttgga
     3601 cggcacggcc gttaaggtaa tcatctaact gcaccgtaaa aattgcggca gcaccgcttt
     3661 cgtcttgtga acattcgttg cccagccaat aatgcaagtc atactgcagg tttccattgc
     3721 gaagttgaac ggttttaagg ataacgtatg catctccagt gaaaaagtcc ccataaaggt
     3781 ttgtcggcac tggcaccaga tcgaactttt ccacgcgcca gatttgcaat ccaggttctt
     3841 ttcccgcttt taaaaattcg ggatgttcaa caaccattcc ccgggaagcg gccgcacttc
     3901 ccccggagcc tccgcttccg cccttgtaca gctcgtccat gccgagagtg atcccggcgg
     3961 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct
     4021 tggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     4081 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     4141 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     4201 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     4261 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     4321 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     4381 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     4441 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     4501 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     4561 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     4621 cctcgccctt gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accgtcgact
     4681 gcagaattcg gttcactaaa cgagcccggt tcactaaacg agctctgctt atatagacct
     4741 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     4801 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     4861 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     4921 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     4981 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     5041 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     5101 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     5161 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     5221 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     5281 aataactagc acgcgttcga agcttgagct cggtcccctc ggggttggga ggtgggtctg
     5341 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     5401 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     5461 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     5521 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     5581 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     5641 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     5701 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     5761 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     5821 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     5881 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     5941 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     6001 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     6061 gtccctcata tctcctcctt atggtgtttt actaaacttt tccatgttct aatcctcatc
     6121 ctgtctactt gccacacaat catcacctgc catctgtttt ccataatccc taatgatctt
     6181 tgcttttctt cttggcacta cttttatgtc actattatct tgtattacta ctgccccttc
     6241 acctttccag aggagctttg ctggtccttt ccaaagtgga tttctgctgt ccctgtaata
     6301 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     6361 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     6421 aaaattgtgg atgaatactg ccatttgtac tgctgtctta agatgttcag cctgatctct
     6481 tacctgtcct ataattttct ttaattcttt attcatagat tctactactc cttgactttg
     6541 gggattgtag ggaatttcca ggtctgaaga tcctgctgct tgctgtgctt ttttcttact
     6601 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     6661 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     6721 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     6781 caggccagta ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     6841 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     6901 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     6961 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     7021 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     7081 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     7141 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     7201 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     7261 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     7321 cccagtcccg cccaggccac gcctcccatg gtaatagcga tgactaatac gtagatgtac
     7381 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     7441 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     7501 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     7561 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     7621 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     7681 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgacc caggtggcac
     7741 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7801 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7861 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7921 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7981 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8041 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8101 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8161 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8221 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8281 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8341 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8401 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8461 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8521 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8581 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8641 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8701 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8761 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8821 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8881 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8941 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9001 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9061 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9121 accagtggct gctgccagtg gcgataagtc gtgtcttacc gg
//
