LOCUS       sequence-408165-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SAFB2_SAFB from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SAFB2_SAFB
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4598..5308,5309..5311,5312..5314))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4640..4661)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4989..5008
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5248..5269
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5311..5320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5396..5599)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5425..5445)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5600..5903
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6160..6204)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6389..6622
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6982..7099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7591..7716
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7763..8020
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8022..8401
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8432..8536
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8537..8605,8606..9397)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8755..8774)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9568..9837,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaat
     1801 aacgacgcgt gaaacgggca tcactcggac gactgccacg tgattgtcct ccaaatcccc
     1861 cttgcatccc gccatgcggg atgggatggc cgcgagaggc tcctccgggc gcgaaagacc
     1921 cgcgtccaga cattcctcca cggttcatca tgtgcccagg cccagaatgt ccagacattg
     1981 agcgctcgcc cccttgccaa cgcttgtgat cacggtccat catccctccg tctgctgtac
     2041 cctgccaact gcgatcatcc tcgcgacgac cgtggtctcc ccagtcgcgg cgtccacgag
     2101 ggggaggagg aagaccgcgc ccttcggaca tacgcttgtc actgccgtag ccaccccaac
     2161 catcacgcga atcgcggccg tgacgttcgg ggccaccatg acgctctggg tagtgttgtc
     2221 cttcgcgctc ccccatcatg gaacgagagc cctcgcggcg gtccacggag tgatctgggt
     2281 aacgaccgcg gtcgcggtgg tcgaaatcgt gaaaacggtc ttggcgatta aaatccgagt
     2341 ggtaacgttc gtctaacgcg gcacgtttag cttccggcca ataggcgtcg tcgcgacggt
     2401 ccaagtcata cggacggcga actgctgggc gacgttcctg ttcgtagcgc agttcttgtt
     2461 gacggcgtaa ctcttcacgt tcacgatgga tacgttcttg ctcacgacga cgttcgtgtt
     2521 ccacatgcat gcgttcacgc tccaggcgtt cgcgttccat gcgttcacgc tctaaacgtt
     2581 gacgctggaa agctaagcgc tcgcgggcaa tctccaagcg ttcgcgctcc tcacgctccc
     2641 attgggcctg catacgttgc tcacgctccg agcgctcacg aacacgactg tggctttctg
     2701 aatcacgtga cttgcgcggt tccttcacct tgtcgaagga gactactgag cgtttctcac
     2761 gagacgcaga cttacggtct tgactttttg atgcacgctc cttcgatcca ctagtcttga
     2821 cactaatgac aggcacccct ttagacttgt ccataactac tgtgcgttcg gtcccgcggc
     2881 ttccagattt agtggcacgt gagcgttcgg acgggcctgg cttctggtcg tcttggtcct
     2941 tagatttttc accgctcccg tcgtccccct ttttagcgtc gtctttacga tcgcatttgt
     3001 cgtcacgttt taagttggtc gaacggtccg aattactaga cttctccttc ttaccgtcag
     3061 agtcgcgttt atccgacgtt tttttgccca ccggttcgtt tttagccttt tccacagaga
     3121 tcattttgcc gtgaagctcg gttttgtgta agtggttgat acatttagtg gcttcctcag
     3181 cggtggacat tgtcacgaag ccgtagcagc gcgctcctgg cgaacgagca ttagtgacta
     3241 ctttggcccc cactactttg ccgtatttgg aaaataaatt cttcagatcc gtcgcgcgcg
     3301 tcgtcgaact caaaccggag acccacaagt tacgaccaga acctgaaccc acgcgcccct
     3361 tttcgtcctt gataattggc ttaatgtctt tttcctcttt aaagctactc attttttggt
     3421 cagcaccttc gctagtgctg gactccttcg gggcgggtgg aacctcatta caggcgtcga
     3481 agtcgaattt acgtccatcc tccttggaat cgcgggcttc tgggctcggc gcctctgtgg
     3541 gggcttcagc cagctcctca gaactcgctt cagctaattc agatgccgca ctactttgtt
     3601 ctacggcggg ctccagaccg actggttcac agtcagtacg ttctccatct cctggctgct
     3661 ctgctggttc acgcttgacg acagccagca ggctgtctgc cttgctgctt tgggcgtggg
     3721 ccgtactttc cgaagcaagg tccaggtccc cttcctcagg gtgcgcggag tcgaacaggt
     3781 cctcttcttc ggccagctta cggtcaggac caacagagct tgtatcttgt gcgaagggtt
     3841 gctctaattc cgaactttct tctttcacag gctccgattt acacgtctca ccaaggatgt
     3901 caaggatctt ctcgttctct ggctccaata atgattcttc aatgtcggga gtaactttga
     3961 agtttaagct ggaggtttcc aatgtattct taaacccctc cccatccacg gcgtgctctg
     4021 gcggttgggc aggtaattga cgcaattgcg ccgcaacgta ctccttggag tcacagaagg
     4081 aatcaagcaa gccgtcagtc ccgtcctcgc caaagtcggg agcactggag ttagcgacct
     4141 ccgtctcgtc taatacggac atgtccatca tacccatatt ttgtaaattc tcaagcgaag
     4201 cctccatatc ttcttgccca tcgcgcgagt cgtcctccag tccgttgtcc tccgtcccct
     4261 cctcttccat tttcaatccc ttcacgcaac gcttggcaga ctttttagaa gtggcctcca
     4321 actcaatccc gatttcgtcg gggtcttgtc cctcttcttt cactgctttc ttcagacgtt
     4381 ccatcagtac gcttttgttc ccccccgtat ctaagttacg tttctttaac tctgcgcgta
     4441 aatcgatcac gcgaagctca ctcaaacgac gtgtcccagt ctctgcgacc ccaggaccca
     4501 agctagccgt ccctggtccg ctgtctcctg accctggaag cgtttcggcc attccccggg
     4561 aagcggccgc acttcccccg gagcctccgc ttccgccctt gtacagctcg tccatgccga
     4621 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     4681 ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     4741 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     4801 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
     4861 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     4921 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     4981 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     5041 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     5101 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     5161 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     5221 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     5281 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggccc
     5341 gcggtaccgt cgactgcaga attcggttca ctaaacgagc ccggttcact aaacgagctc
     5401 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     5461 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     5521 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     5581 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     5641 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     5701 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     5761 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     5821 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     5881 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     5941 taattgatta ctattaataa ctagcacgcg ttcgaagctt gagctcggtc ccctcggggt
     6001 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     6061 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     6121 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     6181 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     6241 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     6301 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     6361 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     6421 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     6481 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     6541 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     6601 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     6661 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
     6721 ttcacttctc caattgtccc tcatatctcc tccttatggt gttttactaa acttttccat
     6781 gttctaatcc tcatcctgtc tacttgccac acaatcatca cctgccatct gttttccata
     6841 atccctaatg atctttgctt ttcttcttgg cactactttt atgtcactat tatcttgtat
     6901 tactactgcc ccttcacctt tccagaggag ctttgctggt cctttccaaa gtggatttct
     6961 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     7021 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     7081 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtactgctg tcttaagatg
     7141 ttcagcctga tctcttacct gtcctataat tttctttaat tctttattca tagattctac
     7201 tactccttga ctttggggat tgtagggaat ttccaggtct gaagatcctg ctgcttgctg
     7261 tgcttttttc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     7321 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     7381 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     7441 ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt tctagctccc tgcttgccca
     7501 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     7561 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     7621 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     7681 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     7741 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     7801 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     7861 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     7921 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
     7981 gagggctcgc cactccccag tcccgcccag gccacgcctc ccatggtaat agcgatgact
     8041 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     8101 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     8161 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     8221 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     8281 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     8341 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     8401 cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8461 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8521 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8581 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8641 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8701 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8761 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8821 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8881 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8941 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9001 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9061 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9121 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9181 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9241 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9301 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9361 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9421 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9481 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgg
//
