LOCUS       sequence-408593-        9306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Wilkins KC, Schroeder T, Gu S, Revalde JL, Floor SN
  TITLE     A novel reporter for helicase activity in translation uncovers DDX3X
            interactions.
  JOURNAL   RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123.
  PUBMED    38697667
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079837"; journalName: "RNA"; date: "2024-07-16- 16"; 
            volume: "30"; issue: "8"; pages: "1041-1057"
COMMENT     Sequence Label: KW57_HART_RAC1
FEATURES             Location/Qualifiers
     source          1..9306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      774..1362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1263..1282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1516..1533
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1650..1671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1686..1691,1692..1709,1710..1716)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1724..1740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1729..1751
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1748..1764
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1748..1764
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1783..1803
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1785..1803
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1904..2283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2284..2487
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2502..2682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2729..2854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3347..3580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3765..3809
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4076..4193
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    4330..4464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(4696..5403,5404..5406,5407..5409))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             complement(join(4696..5403,5404..5406,5407..5409))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(4735..4756)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5084..5103
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5343..5364
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5406..5415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6269..6287)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6537..6556
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6980..7568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7033..7053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7451..7462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7655..7888
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7960..8081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8121..8256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8276..8295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8277..8295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8305..8322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8305..8321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8314..8336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8463..8918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8550..8569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8760..8781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8945..9048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9049..9117,9118..9306,1..603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9267..9286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
       61 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      121 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
      181 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
      241 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
      301 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
      361 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
      421 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
      481 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
      541 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
      601 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
      661 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
      721 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      781 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      841 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      901 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      961 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1021 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1081 cggtcggact gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1141 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1201 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1261 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1321 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1381 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1441 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1501 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     1561 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     1621 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     1681 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1741 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     1801 agggaacaaa agctggagct gcaagcttgg ccattgcata cgttgtatcc atatcataat
     1861 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
     1921 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     1981 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2041 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2101 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2161 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2221 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2281 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2341 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2401 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2461 cggtgggagg tctatataag cagagctcgt ttagtgaacc ggggtctctc tggttagacc
     2521 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2581 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2641 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2701 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2761 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2821 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2881 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2941 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     3001 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3061 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3121 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3181 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3241 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gtacccacca
     3301 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3361 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3421 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3481 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3541 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3601 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3661 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3721 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3781 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3841 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3901 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3961 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     4021 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ttaactttta
     4081 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     4141 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     4201 acgagactag cctcgagaga tctgatcata atcagccata ccacatttgt agaggtttta
     4261 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4321 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaggcaa tagcatcaca
     4381 aatttcacaa ataaggcatt tttttcactg cattctagtt ttggtttgtc caaactcatc
     4441 aatgtatctt atcatgtctg gatctcaaat ccctcggaag ctgcgcctgt cttaggttgg
     4501 agtgatacat ttttatcact tttacccgtc tttggattag gcagtagctc tgacggccct
     4561 cctgtcttag gttagtgaaa aatgtcactc tcttacccgt cattggctgt ccagcttagc
     4621 tcgcagggga ggtggtctgg atccaccggt ccggtttaat taacgtagca cgcgtttagt
     4681 catctagcta gcagtgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
     4741 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
     4801 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     4861 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
     4921 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
     4981 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     5041 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
     5101 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     5161 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt
     5221 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     5281 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
     5341 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     5401 gctcaccatg gtggctagcg gttcactaaa cgaactctgc ttatataggc ctcccaccgt
     5461 acacgcctac cctcgagaag cttgatatcg aattccacgg ggttggggtt gcgccttttc
     5521 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     5581 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     5641 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     5701 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     5761 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     5821 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     5881 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcagtt ttcctcagct
     5941 ttgggtggtg gccgctgccg ggcatcggct tccagtccgc ggagggcgag gcggcgtgga
     6001 cagcggcccc ggcacccagc gccccgccgc ccgcaagccg cgcgcccgtc cgccgcgccc
     6061 cgagcccgcc gcttcctatc tcagcgccct gccgccgccg ccgcggccca gcgagcggcc
     6121 ctgatggttt caaaaggtga agaagataac atggccatca tcaaggagtt catgcgcttc
     6181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     6241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     6301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     6361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     6421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     6481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     6541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agagaatgta ccccgaggac
     6601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     6661 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     6721 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     6781 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gaagcttagc
     6841 catggcttcc cgccggaggt ggaggagcag gatgatggcg cgctgcccat gtcttgtgcc
     6901 caggagagcg ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtag
     6961 agtcgagtct agagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     7021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7141 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7201 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7261 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7321 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7381 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7441 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7501 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7561 ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     7621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7681 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7861 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7921 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7981 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8041 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     8101 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8161 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8221 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8281 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8341 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8401 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8461 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8521 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8581 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8641 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8701 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8761 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8821 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8881 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     8941 gtgtgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     9001 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     9061 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     9121 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     9181 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9241 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     9301 gggcaa
//
