LOCUS       sequence-408621-       10118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-q ONE-GO
FEATURES             Location/Qualifiers
     source          1..10118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             591..611
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             1044..1559
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    1573..2161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1626..1646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2044..2055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2368..3001
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3129..3145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3129..3145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3142..3164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3153..3169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3177..3183,3184..3201,3202..3207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3222..3243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3360..3377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3531..4119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3611..3630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4290..5081,5082..5150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4913..4932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5151..5255)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5303..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5340..5359)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5394..5413
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5605..6238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6285..6410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6907..7140
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7325..7369
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7632..7747
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        7789..8236
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(8659..8661,8662..8664,8665..9375)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8704..8725)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8965..8984)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9312..9333
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(10112..10118,1..568,569..571,572..580)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
       61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataagct tgccacaacc cgtaccaaag atggggagta
      601 gcaagagcaa gacgtcgaat tccggaatca agcttaagta cctggaggac cggggcgagg
      661 tgacttttga gaagatcttc tcccagaagc tggggtacct gcttttccga gacttctgcc
      721 tgaagcacct ggaggaggcc aagcccttgg tagagttcta cgaggagatc aagaaatacg
      781 agaagctgga gacagaggag gagcgcctgg tctgcagccg agagatcttc gacacgtaca
      841 tcatgaagga gctgctggcc tgctcacatc ctttctcgaa gagcgccatt gagcacgtcc
      901 agggccatct ggtgaagaag caggtgcctc cggatctctt ccagccatat attgaagaaa
      961 tttgccagaa cctccgagga gacgtgttcc agaaattcat cgagagcgat aaattcacac
     1021 ggttttgcca gtggaaggga tccatggtct tcacactcga agatttcgtt ggggactggc
     1081 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     1141 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     1201 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     1261 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     1321 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     1381 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     1441 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     1501 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaaa
     1561 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1621 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1681 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     1741 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     1801 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     1861 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     1921 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     1981 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2041 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2101 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2161 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata
     2221 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     2281 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     2341 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     2401 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     2461 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     2521 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     2581 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     2641 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     2701 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     2761 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     2821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3001 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     3061 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     3121 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3241 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3301 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3361 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3421 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3481 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3541 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3601 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3661 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3721 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3781 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3841 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3901 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3961 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4021 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4081 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4141 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4201 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4261 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4321 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4381 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4441 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4501 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4561 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4621 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4681 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4741 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4801 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4861 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4921 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4981 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5041 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5101 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5161 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5221 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5281 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     5341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5401 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     5461 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     5521 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     5581 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     5641 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     5701 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5761 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     5821 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     5881 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     5941 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     6001 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     6061 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6121 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6181 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6241 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     6301 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     6361 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     6421 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     6481 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     6541 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     6601 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     6661 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     6721 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     6781 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     6841 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     6901 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     6961 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7021 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7081 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7141 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7201 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7261 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7321 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     7381 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     7441 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     7501 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     7561 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     7621 acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     7681 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     7741 aaaattttcg ggtttattac agggacagca gagatccagt ttatcgatag tgcaagtggg
     7801 ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact
     7861 ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg
     7921 aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc
     7981 gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact
     8041 cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg
     8101 cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg
     8161 ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc
     8221 gtgcctgaga gcgcagctcg agactagttc tagagcggcc gcggatccat gactctggag
     8281 tccatcatgg cgtgctgcct gagcgaggag gccaaggaag cccggaggat caacgacgag
     8341 atcgagcggc agctgcgcag ggacaagcgc gacgcccgcc gggaactcaa gctgctgctg
     8401 ctggggacag gggagagtgg caagagcacc ttcatcaagc agatgaggat catccacggg
     8461 tcgggctact ctgacgaaga caagcgcggc ttcaccaagc tggtgtatca gaacatcttc
     8521 acggccatgc aggccatgat cagagcgatg gacacactca agatcccata caagtatgaa
     8581 cacaataagg ctcatgcaca attggttcga gaggttgatg tggagaaggt gtctgctttt
     8641 tcaggaggtg gcggatccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     8701 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     8761 gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg
     8821 cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc
     8881 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     8941 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     9001 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     9061 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
     9121 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     9181 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     9241 ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga ccccaacgag
     9301 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     9361 gacgagctgt acaagtctgg cggtggagga tccgagaatc catatgtaga tgcaataaag
     9421 agcttgtgga atgatcctgg aatccaggag tgctacgaca gacgacggga atatcagtta
     9481 tctgactcta ccaaatacta tctgaatgac ttggaccgtg tagccgaccc tgagtacatg
     9541 ccgacagagc aagacgtgct tagagttcga gtccccacta cagggatcat cgaatacccc
     9601 tttgacttac aaagtgtcat tttcagaatg gtcgatgtag ggggccaaag gtcagagaga
     9661 agaaaatgga tacactgctt tgaaaatgtc acctccatca tgtttctagt agcgcttagc
     9721 gaatatgatc aagttcttgt ggagtcagac aatgagaacc gcatggagga gagcaaagca
     9781 ctctttagaa caattatcac ctacccctgg ttccagaact cctctgtgat tctgttctta
     9841 aacaagaaag atcttctaga ggagaaaatc atgtattccc acctagtcga ctacttccca
     9901 gaatatgatg gaccccagag agatgcccag gcagctcgag aattcatcct gaaaatgttc
     9961 gtggacctga accccgacag tgacaaaatc atctactccc acttcacgtg cgccacagat
    10021 accgagaaca tccgcttcgt ctttgcagcc gtcaaggaca ccatcctgca gctgaacctg
    10081 aaggagtaca atctggtcta agcggccgcc gcccctct
//
