LOCUS       sequence-408845-        9463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0696
FEATURES             Location/Qualifiers
     source          1..9463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     304..323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     557..574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    691..712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(727..732,733..750,751..757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    765..781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     770..792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     789..805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     789..805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     824..844
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        826..844
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        872..1098
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1099..1279
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1326..1451
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1944..2177
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2362..2406
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2637..2847
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2659..2855
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2706..2841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2887..3489
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2887..2906)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3383..3403
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3532..3649
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3787..4965
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4018..4956
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4913..4933
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6187..6775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6240..6260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6658..6669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6847..7080
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7158..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7195..7214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7249..7268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7319..7454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7474..7493)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7475..7493)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7503..7520)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7503..7519)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7512..7534)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7661..8116
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7748..7767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7958..7979
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8142..8246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8247..8315,8316..9107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8465..8484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9278..9463,1..403)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
       61 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      421 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      481 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      541 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      601 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      661 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      721 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      781 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      841 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      901 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      961 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     1021 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     1081 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     1141 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     1201 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     1261 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     1321 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     1381 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     1441 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     1501 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     1561 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1621 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1681 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1741 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1801 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1861 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1921 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1981 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     2041 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     2101 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     2161 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     2221 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     2281 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     2341 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     2401 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     2461 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     2521 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2581 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     2641 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
     2701 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     2761 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     2821 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
     2881 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2941 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     3001 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     3061 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     3121 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     3181 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     3241 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     3301 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     3361 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     3421 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     3481 gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3541 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3601 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3661 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
     3721 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     3781 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     3841 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     3901 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3961 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     4021 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     4081 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     4141 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     4201 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     4261 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     4321 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     4381 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     4441 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     4501 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     4561 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     4621 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     4681 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     4741 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     4801 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     4861 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     4921 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcaccgccac
     4981 cctggatccg ccggccgtgg tcgccgagtc ggtgtcgtcc ttgaccatcg ccgacgcgtt
     5041 cattgcagcc ggcgagagct cagctccgac cccgccgcgc cccgcgcttc ccaggaggtt
     5101 catctgctcc ttccctgact gcagcgccaa ttacagcaaa gcctggaagc ttgacgcgca
     5161 cctgtgcaag cacacggggg agagaccatt tgtttgtgac tatgaagggt gtggcaaggc
     5221 cttcatcagg gactaccatc tgagccgcca cattctgact cacacaggag aaaagccgtt
     5281 tgtttgtgca gccaatggct gtgatcaaaa attcaacaca aaatcaaact tgaagaaaca
     5341 ttttgaacgc aaacatgaaa atcaacaaaa acaatatata tgcagttttg aagactgtaa
     5401 gaagaccttt aagaaacatc agcagctgaa aatccatcag tgccagcata ccaatgaacc
     5461 tctattcaag tgtacccagg aaggatgtgg gaaacacttt gcatcaccca gcaagctgaa
     5521 acgacatgcc aaggcccacg agggctatgt atgtcaaaaa ggatgttcct ttgtggcaaa
     5581 aacatggacg gaacttctga aacatgtgag agaaacccat aaagaggaaa tactatgtga
     5641 agtatgccgg aaaacattta aacgcaaaga ttaccttaag caacacatga aaactcatgc
     5701 cccagaaagg gatgtatgtc gctgtccaag agaaggctgt ggaagaacct atacaactgt
     5761 gtttaatctc caaagccata tcctctcctt ccatgaggaa agccgccctt ttgtgtgtga
     5821 acatgctggc tgtggcaaaa catttgcaat gaaacaaagt ctcactaggc atgctgttgt
     5881 acatgatcct gacaagaaga aaatgaagct caaagtcaaa aaatctcgtg aaaaacggag
     5941 tttggcctct catctcagtg gatatatccc tcccaaaagg aaacaagggc aaggcttatc
     6001 tttgtgtcaa aacggagagt cacccaactg tgtggaagac aagatgctct cgacagttgc
     6061 agtacttacc cttggctagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     6121 gctttatata tcttgtggaa aggacgacac tgtctcatcc tgtctggctg gacgcgttaa
     6181 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     6781 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6841 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6901 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6961 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7021 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7081 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7141 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7201 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7261 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7381 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7441 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7501 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7561 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7621 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     7681 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7741 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7801 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7861 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7921 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7981 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8041 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8101 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8161 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8221 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8281 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8341 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8401 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8461 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8521 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8581 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8641 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8701 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8761 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8821 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8881 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8941 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9001 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9061 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9121 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaa
//
