LOCUS       sequence-408848-        9730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0811
FEATURES             Location/Qualifiers
     source          1..9730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             212..392
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    439..564
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1057..1290
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1475..1519
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1750..1960
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1772..1968
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1819..1954
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2000..2602
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2000..2019)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2496..2516
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2645..2762
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2900..4078
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3131..4069
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4026..4046
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4089..4098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5567..6155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5620..5640)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6038..6049)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6227..6460
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6538..6659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6575..6594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6629..6648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6699..6834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6854..6873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6855..6873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6883..6900)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6883..6899)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6892..6914)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7041..7496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7128..7147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7338..7359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7522..7626
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7627..7695,7696..8487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7845..7864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8658..9246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9147..9166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9400..9417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9534..9555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9570..9575,9576..9593,9594..9600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9608..9624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9613..9635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9632..9648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9632..9648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9667..9687
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9669..9687
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9715..9730,1..211)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
       61 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
      121 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
      181 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
      241 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      301 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      361 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      421 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      481 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      541 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      601 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      661 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
      721 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
      781 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
      841 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
      901 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
      961 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1021 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1081 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1141 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1201 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1261 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1321 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1381 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1441 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1501 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1561 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1621 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1681 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     1741 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     1801 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1861 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1921 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     1981 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     2041 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     2101 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     2161 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     2221 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     2281 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     2341 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     2401 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     2461 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     2521 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     2581 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     2641 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2701 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2761 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     2821 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     2881 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2941 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     3001 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3061 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3121 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3181 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3241 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3301 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3361 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3421 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3481 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3541 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3601 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3661 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3721 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3781 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3841 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3901 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     3961 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4021 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     4081 ctagcaccgc caccatggag ttggcgactc gctaccagat ccctaaagaa gtggctgaca
     4141 tctttaacgc ccccagtgat gatgaagagt ttgttggctt ccgagatgat gttcccatgg
     4201 aaaccctctc gtcagaggag agctgcgata gttttgactc actagagtca gggaaacagc
     4261 aggatgtgcg ctttcattcc aaatacttca cagaagagct aagaagaatt tttatagagg
     4321 acactgactc agagactgag gattttgcag gatttacgca gagtgatctg aatggaaaga
     4381 ctaacccaga agtaatggtc gtggagtcag atttgagtga tgatggcaaa gcatctttgg
     4441 tgagcgagga agaggaagat gaagaagaag ataaggctac ccctagaaga agcaggtcta
     4501 gaagaagtag tattggtctt cgagtagcct ttcagttccc caccaagaag ctggccaaca
     4561 aaccagataa aaacagttct tccgagcagt tgttttctag cgcacgctta cagaatgaga
     4621 aaaaaacaat tcttgaaaga aagaaagact gtagacaggt gatacaaagg gaagattcta
     4681 cctctgagtc tgaggatgac tctcgggatg agagccagga gagttcagat gctttgctga
     4741 aaaggaccat gaacatcaag gagaacaaag ccatgcttgc ccagttattg gcggaattga
     4801 actcgatgcc agatttcttc ccagtacgaa ccccaacctc agcttctagg aagaagacag
     4861 tgaggcgggc cttctcggag ggacagatca cgcggcgtat gaacccaacc cggagtgcgc
     4921 ggcctcctga gaagtttgct ctagagaact tcactgtctc agccgctaaa tttgcggaag
     4981 agttttacag cttccgaaga aggaagacaa ttggggggaa atgccgggag tacagacgac
     5041 gtcaccgtat atcttctttt cggccagtgg aggatatcac cgaagaggac ttagaaaatg
     5101 ttgccataac tgttcgagat aaaatctatg ataaagttct gggtaacacg tgccatcagt
     5161 gtcgacaaaa gaccatcgac accaagacag tgtgtcggaa ccagggttgc tgtggtgtgc
     5221 gaggacagtt ctgtggacca tgcctgcgga accgctatgg ggaggatgtc agatcggcat
     5281 tgctggaccc ggattgggtg tgtcccccct gtcgtgggat ctgcaattgc agctactgtc
     5341 ggaagcgtga cggccgctgt gccacaggaa tcctcattca tctggccaag ttttatggtt
     5401 atgacaatgt taaggaatat ctggagagct tacaaaagga gctggtagaa gacaattagt
     5461 aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     5521 aggacgacgc ctacttccac agtcggaatc cacgcgttaa gtcgacaatc aacctctgga
     5581 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5641 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5701 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5761 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5821 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5881 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5941 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6001 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6061 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6121 gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac
     6181 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6241 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     6301 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6361 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6421 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     6481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     6541 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6601 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6661 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6721 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6781 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     6841 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6901 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     6961 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     7021 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7381 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7441 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     7501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7861 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8161 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8341 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     9001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     9061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     9481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     9661 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     9721 cttatgcaat
//
