LOCUS       sequence-408942-       11535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, 
            human and CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11535)
  AUTHORS   Pizzato HA, Alonso-Guallart P, Woods J, Connelly JP, Fehniger TA, 
            Atkinson JP, Pruett-Miller SM, Monsma FJ Jr, Bhattacharya D
  TITLE     Engineering human pluripotent stem cell lines to evade xenogeneic 
            transplantation barriers.
  JOURNAL   Stem Cell Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003.
  PUBMED    38215755
REFERENCE   2  (bases 1 to 11535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2023 Dec 22:S2213-6711(23)00496-4. doi: 
            10.1016/j.stemcr.2023.12.003."
COMMENT     Sequence Label: AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G
            SCT-T2A-hCD4...
FEATURES             Location/Qualifiers
     source          1..11535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(101..123)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     223..242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     436..458
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     450..467
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     451..467
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1904..3082
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2135..3073
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3030..3050
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3099..3118
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3099..3117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3208..3217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4354..4410
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5851..5904
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6883..6942
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7336..7401
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(8216..8233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8222..8446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9386..9402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9386..9402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9399..9421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9410..9426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9434..9440,9441..9458,9459..9464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9479..9500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9617..9634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9788..10376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9868..9887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10547..11338,11339..11407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11170..11189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11408..11512)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
      121 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
      181 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
      241 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
      301 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
      361 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
      421 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgccgtga
      481 cgtcagcacg ccgggcgggg accgggagat ccttggggcg gtggggggcc agcggcagtt
      541 cccggcggcc cccggggcgg gcgggcgggc gggtggtggc ggcggttggg gctcggcgct
      601 cgctcgctcg ctgggcgggc gggcggtgcg atgtccggag aggatggccc ggcggctggc
      661 ccgggggcgg cggcggcggc tgcccgggag cggcgacggg agcagctgcg gcagtggggg
      721 gcgcgggcgg gcgccgagcc tggccccgga gagcgccgcg cccgcaccgt ccgcttcgag
      781 cgcgccgccg agttcctggc ggcctgtgcg ggcggcgacc tggacgaggc gcgtctgatg
      841 ctgcgcgccg ccgaccctgg ccccggcgcc gagctcgacc ccgccgcgcc gccgcccgcc
      901 cgcgccgtgc tggactccac caacgccgac ggtatcagcg ccctgcacca ggtcagcgcc
      961 ccccgcccgg cgtctcccgg ggccaggtcc accctctgct gcgccacctg gggcatcctc
     1021 cttccccgtt gccagtctcg atccgccccg tcgttcctgg ccctgggctt tgccacccta
     1081 tgctgacacc ccgtcccagt cccccttacc attccccttc gaccacccca cttccgaatt
     1141 ggagccgctt caactggccc tgggcttagc cactctgtgc tgaccactct gccccaggcc
     1201 tccttaccat tccccttaaa caccggtggg gaggtggtcc ctgctgttac gccaatgata
     1261 acccccgcca gaaaaatctt agtagccttc cctttttgtt ttccgtgccc caactcggcg
     1321 gattgactcg gccccttccg gaaacacccg aatcaacttc tagtcaaatt attgttcacg
     1381 ccgcaatgac ccacccctgg cccgcgtctg tggaactgac ccctggtgta caggagagtt
     1441 cgctgctgaa agtggtccca aaggggtact agtttttaag ctcccaactc cccctccccc
     1501 agcgtctgga ggattccaca ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt
     1561 tttggcgcca gccgctgagg ctgccaagca gaaaagccac cgctgaggag actccggtca
     1621 ctgtcctcgc cccgcctccc ccttccctcc ccttggggac caccgggcgc cacgccgcga
     1681 acgttaagtg ccgcggtcgt cggcgcctcc gccctccccc ttgggcccca attcccagcg
     1741 ggcgcggcgc gcggcccctc cccccgccgg gcgcgcgccc gctgccccgc ccttcgtggc
     1801 cgcccggcgt gggcggtgcc acccctcccc ccggcggccc cgcgcgcagc tcccggctcc
     1861 ctcccccttc ggatgtggct tgagctgtag gcgcggaggg ccgggctccg gtgcccgtca
     1921 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     1981 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2041 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2101 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2161 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2221 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2281 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2341 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2401 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2461 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2521 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2581 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2641 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2701 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     2761 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     2821 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     2881 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     2941 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3001 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3061 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     3121 acccaagctg gctaggtaag cttggtaccg agctcggatc cactagtcca gtgtggtgga
     3181 attctgcaga tatccagcac agtggcggcc gccatggaac cccctggaag gagagagtgc
     3241 cctttccctt cctggagatt tcccggactg ctgctggctg ccatggtcct gctgctgtac
     3301 agcttcagcg atgcttgtga ggagcctccc acattcgagg ccatggaact cattggcaag
     3361 cctaagccct actacgaaat cggcgagagg gtcgattaca agtgtaagaa gggctatttc
     3421 tacatccccc ccctggccac acacaccatc tgcgatagaa accacacatg gctccctgtg
     3481 tccgacgatg cttgctatag ggagacctgc ccttacatca gggaccctct gaatggacag
     3541 gccgtgcctg ctaatggcac ctacgaattc ggctatcaaa tgcatttcat ctgcaatgag
     3601 ggctactacc tgatcggcga agagattctc tactgtgagc tcaagggaag cgtcgctatc
     3661 tggagcggaa agcctcccat ctgcgagaaa gtgctgtgca cccccccccc taaaatcaaa
     3721 aatggcaagc acaccttcag cgaggtggaa gtgttcgaat atctggatgc cgtgacctac
     3781 agctgtgatc ctgctcctgg acccgatccc tttagcctga ttggcgagag cacaatttac
     3841 tgcggagaca actccgtgtg gagcagagcc gcccccgagt gtaaggtcgt gaaatgtagg
     3901 ttccctgtcg tcgagaatgg caaacagatc agcggcttcg gcaaaaagtt ctactataaa
     3961 gctaccgtga tgtttgagtg cgacaaggga ttctacctcg atggcagcga tacaatcgtg
     4021 tgcgatagca actccacatg ggacccccct gtccccaaat gcctgaaagt gagcaccagc
     4081 tccacaacca agtcccccgc ctcctccgcc tccggcccca gacctaccta taagccccct
     4141 gtgtccaact accccggcta ccccaaaccc gaggaaggca ttctggactc cctggatgtg
     4201 tgggtgattg ccgtcatcgt cattgctatt gtggtcggag tcgctgtgat ctgtgtcgtg
     4261 ccctatagat acctgcagag aaggaaaaag aagggcacct acctgaccga cgagacccac
     4321 agagaagtga agtttaccag cctgggatcc ggagccacca actttagcct gctcaaacaa
     4381 gccggcgacg tggaagagaa ccccggacct atgtccagat ccgtggccct ggctgtgctg
     4441 gctctgctgt ccctgagcgg cctcgaggcc aaaggacccc ccgctgctct gacactgggc
     4501 tgtggcgctt ccggaggcgg cggcagcgga ggcggaggca gcattcagag gacccctaag
     4561 atccaggtgt actccaggca tcctgccgag aacggcaaat ccaatttcct caattgctac
     4621 gtgtccggct tccatccctc cgacatcgaa gtggacctcc tcaagaacgg agagagaatc
     4681 gaaaaggtgg agcactccga cctgtccttc tccaaagact ggtccttcta cctgctgtat
     4741 tacacagagt ttacccctac cgagaaggac gagtacgcct gtagagtgaa tcacgtgaca
     4801 ctgtcccagc ccaagattgt gaaatgggac agggacatgg gcggcggagg aagcggaggc
     4861 ggcggaagcg gcggcggcgg cagcggcggc ggcggctccg gctcccacag catgaggtac
     4921 ttttccgccg ccgtgtccag gcccggcagg ggcgaaccta gatttatcgc tatgggctac
     4981 gtcgacgaca cccagtttgt gaggttcgac agcgactccg cctgccccag aatggaacct
     5041 agagcccctt gggtcgagca ggaaggcccc gagtactggg aggaggagac caggaatacc
     5101 aaggcccacg ctcagaccga caggatgaat ctgcagaccc tgaggggatg ctacaaccaa
     5161 agcgaggcca gctcccacac cctgcagtgg atgatcggct gtgatctggg atccgacggc
     5221 aggctcctga gaggctatga gcagtacgcc tacgatggca aggattacct ggctctcaac
     5281 gaggacctca gatcctggac agccgccgat acagccgctc agatctccaa gaggaagtgc
     5341 gaggctgcca atgtcgctga gcagaggagg gcctacctgg aaggaacctg cgtcgaatgg
     5401 ctgcacaggt acctggaaaa cggcaaggaa atgctccaga gggccgaccc tcctaagaca
     5461 cacgtcacac accaccccgt gttcgattat gaggccacac tcaggtgctg ggctctcggc
     5521 ttttatcccg ccgagattat cctgacctgg cagagagatg gcgaggatca gacccaggac
     5581 gtcgagctgg tggagacaag gcctgccggc gacggaacat ttcagaaatg ggctgctgtg
     5641 gtggtgccct ccggcgagga acagaggtac acatgccacg tgcagcatga aggactcccc
     5701 gagcccctga tgctgagatg gaagcagtcc tccctgccta caatccccat catgggcatc
     5761 gtggctggcc tcgtcgtcct cgctgctgtg gtgaccggcg ctgccgtcgc cgccgtgctg
     5821 tggagaaaga agtcctccga tggaagcggc gagggaaggg gaagcctgct gacatgcggc
     5881 gatgtggagg agaaccctgg acctatgtgg cctctcgtcg ctgccctgct gctgggatcc
     5941 gcctgctgtg gatccgccca gctcctgttt aataaaacaa agagcgtgga gtttaccttt
     6001 tgtaacgata ccgtggtgat cccctgcttc gtcaccaaca tggaagccca gaataccacc
     6061 gaagtgtatg tcaagtggaa gttcaaaggc agggacattt acaccttcga tggcgctctg
     6121 aacaaaagca ccgtgcccac agatttcagc tccgccaaga tcgaagtgtc ccagctgctg
     6181 aagggcgatg ctagcctgaa aatggacaaa tccgacgccg tgagccacac cggcaactat
     6241 acctgcgagg tcacagaact gacaagggaa ggcgagacaa tcatcgagct gaaatacaga
     6301 gtggtgtcct ggttctcccc taacgagaac attctgatcg tgattttccc tatctttgcc
     6361 attctgctgt tctggggcca gtttggcatc aagaccctga agtatagatc cggcggcatg
     6421 gatgagaaaa ccattgccct cctggtggct ggcctggtca ttaccgtgat cgtgatcgtg
     6481 ggcgctatcc tcttcgtccc tggcgagtac agcctgaaaa acgccaccgg actgggactg
     6541 atcgtgacct ccacaggaat cctgattctg ctgcactatt acgtgtttag caccgctatc
     6601 ggcctgacct cctttgtgat cgccatcctg gtgatccagg tcatcgctta tatcctggcc
     6661 gtcgtcggac tgagcctctg tatcgccgcc tgtatcccca tgcatggccc cctcctcatc
     6721 agcggactct ccattctggc cctggcccaa ctcctgggcc tcgtgtacat gaagttcgtg
     6781 gcctccaacc aaaaaacaat ccagcctccc agaaaggccg tcgaggagcc cctgaacgct
     6841 tttaaggaga gcaaaggcat gatgaacgat gagggatccg gacagtgtac caactatgcc
     6901 ctcctgaaac tggctggcga cgtggagagc aatcctggac ctatgggcat ccagggcggc
     6961 tccgtcctgt tcggcctcct cctcgtcctg gccgtctttt gccattccgg ccattccctc
     7021 cagtgctaca actgtcctaa ccccaccgcc gactgtaaga cagctgtgaa ctgtagcagc
     7081 gacttcgatg cttgcctgat cacaaaggct ggactgcagg tgtacaataa gtgctggaag
     7141 ttcgagcact gcaacttcaa tgacgtgaca accaggctga gggaaaacga gctgacctac
     7201 tactgctgca aaaaggacct gtgcaatttc aatgagcagc tggagaatgg cggcacatcc
     7261 ctgagcgaga aaaccgtgct gctgctggtg acccctttcc tggccgccgc ttggtccctg
     7321 caccccggct ccggagtgaa gcagaccctc aacttcgatc tcctcaaact ggccggagac
     7381 gtcgaaagca atcctggacc tatgatcgag caggacggac tccatgctgg aagccctgcc
     7441 gcttgggtcg agaggctctt tggatacgac tgggctcagc agacaatcgg ctgcagcgat
     7501 gccgccgtgt ttagactcag cgcccagggc agacctgtgc tgtttgtgaa gaccgacctg
     7561 agcggagctc tcaatgagct ccaagatgaa gccgccagac tgagctggct cgccaccaca
     7621 ggcgtgcctt gtgctgccgt gctggacgtc gtcacagagg ccggaaggga ttggctgctc
     7681 ctgggcgaag tccccggaca ggatctgctg agctcccatc tggcccctgc cgaaaaggtc
     7741 tccattatgg ccgatgccat gaggaggctg cacaccctgg atcccgctac ctgtcccttt
     7801 gaccatcagg ccaagcacag gattgagagg gccagaacaa gaatggaggc cggcctggtc
     7861 gaccaggatg atctggacga ggaacaccag ggcctcgctc ctgccgagct ctttgccaga
     7921 ctcaaggcca ggatgcctga cggcgaagat ctggtggtga cccatggaga tgcctgtctc
     7981 cccaacatca tggtggaaaa cggcaggttc tccggattca ttgactgcgg caggctcggc
     8041 gtggccgaca ggtatcagga catcgccctg gctaccagag atatcgctga ggagctggga
     8101 ggagagtggg ccgatagatt tctcgtgctc tatggcattg ccgctcccga cagccagaga
     8161 attgccttct acagactgct cgatgagttt ttttgagttt aaacccgctg atcagcctcg
     8221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     8281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     8341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     8401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     8461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc atctagcggc
     8521 cgatcaggcc cgacctactc tcttccgcat tggagtcgct ttaactggcc ctggctttgg
     8581 cagcctgtgc tgacccatgc agtcctcctt accatccctc cctcgacttc ccctcttccg
     8641 atgttgagcc cctccagccg gtcctggact ttgtctcctt ccctgccctg ccctctcctg
     8701 aacctgagcc agctcccata gctcagtctg gtctatctgc ctggccctgg ccattgtcac
     8761 tttgcgctgc cctcctctcg cccccgagtg cccttgctgt gccgccggaa ctctgccctc
     8821 taacgctgcc gtctctctcc tgagtccgga ccactttgag ctctactggc ttctgcgccg
     8881 cctctggccc actgtttccc cttcccaggc aggtcctgct ttctctgacc tgcattctct
     8941 cccctgggcc tgtgccgctt tctgtctgca gcttgtggcc tgggtcacct ctacggctgg
     9001 cccagatcct tccctgccgc ctccttcagg ttccgtcttc ctccactccc tcttcccctt
     9061 gctctctgct gtgttgctgc ccaaggatgc tctttccgga gcacttcctt ctcggcgctg
     9121 caccacgtga tgtcctctga gcggatcctc cccgtgtctg ggtcctctcc gggcatctct
     9181 cctccctcac ccaaccccat gccgtcttca ctcgctgggt tcccttttcc ttctccttct
     9241 ggggcctgtg ccatctctcg tttcttagga tggccttctc cgacggatgt ctcccttgcg
     9301 tcccgcctcc ccttcttgta ggcctgcatc aggccaaatt ggccgtcgac tgcagaggcc
     9361 tgcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9421 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9481 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9541 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     9601 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     9661 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     9721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     9781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     9841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     9901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9961 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10021 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10081 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10141 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10201 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10261 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10321 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10381 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10441 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    10501 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    10561 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    10621 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    10681 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    10741 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    10801 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    10861 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    10921 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10981 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11041 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11101 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11161 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11221 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11281 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11341 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11401 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    11461 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    11521 cccgaaaagt gccac
//
