LOCUS       sequence-409136-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with mOrange reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPOW
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5041..5540
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5076..5099)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5473..5492
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(6329..6349)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6747..6758)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6948..7581
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7710..7726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7710..7726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7723..7745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7734..7750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7758..7764,7765..7782,7783..7788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7803..7824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7941..7958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8112..8700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8192..8211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8871..9662,9663..9731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9494..9513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9732..9836)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9884..10018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9921..9940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9975..9994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgttcca tgtccttata tggactcatc tttgcctatt
     2221 gcgacacaca ctcaatgaac acctactacg cgctgcaaag agccccgcag gcctgaggtg
     2281 cccccacctc accactcttc ctatttttgt gtaaaaatcc agcttcttgt caccacctcc
     2341 aaggaggggg aggaggagga aggcaggttc ctctaggctg agccgaatgc ccctctgtgg
     2401 tcccacgcca ctgatcgctg catgcccacc acctgggtac acacagtctg tgattcccgg
     2461 agcagaacgg accctgccca cccggtcttg tgtgctactc agtggacaga cccaaggcaa
     2521 gaaagggtga caaggacagg gtcttcccag gctggctttg agttcctagc accgccccgc
     2581 ccccaatcct ctgtggcaca tggagtcttg gtccccagag tcccccagcg gcctccagat
     2641 ggtctgggag ggcagttcag ctgtggctgc gcatagcaga catacaacgg acggtgggcc
     2701 tagacccagg ctgtgtagac ccagcccccc cgccccgcag tgactaggtc acccactaac
     2761 gccccaggcc tggtcttggc tgggcgtgac tgttaccctc aaaagcaggc agctccaggg
     2821 taaaaggtgc cctgccctgt agagcccacc ttccttccca gggctgcggc tgggtaggtt
     2881 tgtagccttc atcacgggcc acctccagcc actggaccgc tggcccctgc cctgtcctgg
     2941 ggagtgtggt cctgcgactt ctaagtggcc gcaagccacc tgactccccc aacaccacac
     3001 tctacctctc aagcccaggt ctctccctag tgacccaccc agcacattta gctagctgag
     3061 ccccacagcc agaggtcctc aggccctgct ttcagggcag ttgctctgaa gtcggcaagg
     3121 gggagtgact gcctggccac tccatgccct ccaagagctc cttctgcagg agcgtacaga
     3181 acccagggcc ttggcacccg tgcagaccct ggcccacccc acctgggcgc tcagtgccca
     3241 agagatgtcc acaactagga tgtcccgcgg tgggtggggg gccagagaga cgggcaggcc
     3301 gggggcaggc ctggccatgc ggggccgaac cgggcactgc ccagcgtggg gcgcgggggc
     3361 cacggcgcgc gcccccagcc ccccggccca gcaccccaag gcggccaacg ccaaaactct
     3421 ccctcctcct cttcctcaat ctcgctctcg ctcttttttt ttttcgcaaa aggaggggag
     3481 agggggtaaa aaaatgctgc actgtgcggc gaagccggtg agtgagcggc gcggggccaa
     3541 tcagcgtgcg ccgttccgaa agttgccttt tatggctcga acggccgcgg cggcgcccta
     3601 taaaacccag cggcgcgacg cgccaccacc gccgagaccg cgtccgcccc gcgagcacag
     3661 agcctcgcct ttgccgatcc gccgcccgtc caggctcgca tctctccttc acgcgcccgc
     3721 cgccttacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
     3781 ggtgcctcct gaactacgtc cgccgtctag gtaagtttag agctcaggtc gagaccgggc
     3841 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
     3901 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatca
     3961 gcgcttcgac tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat
     4021 cgttgcctgc acatcttgga aacacttgct gggattactt cgacttctta acccaacaga
     4081 aggctcgaga aggtatattg ctgttgacag gatccacatt aacaacttta agtccagcaa
     4141 tttgagttaa gggtgttgct ctcaatgatt tcactaatgg ttcaattttt aatttctttt
     4201 cttctggttc aaaattcaag tttcaagtga aagtgtaata tgcacccatt tctttcaata
     4261 aatcttctaa atagtccact aatgttttat tttgtttttt acaaaatcaa gcagcctctg
     4321 ctatcaatat agaaccttgt attccatctt tatctctagc tgagtcatca attacatatc
     4381 cataactttc ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt
     4441 ctctacccat tcatttcaat ccagttaaag tttttacaat cttaactcca tatttttcct
     4501 gagcgattct atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg
     4561 gaatgctatt taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt
     4621 gatttccatc taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag
     4681 catctgggtc attcataata ataatatctg catcatgttt aataccatat tcaagcggta
     4741 tttttcatgc aggatcaaat tctggatttg gatttacaac atttttcaat gtttcatctt
     4801 caaatgcacg atcttcaccc tcaataacgt tatatcctga ttcacgtaat ctttttgggg
     4861 taaatttagt tcctgttcca ttaactgcgc taaaaatagg gccctgcctc ggacttcaag
     4921 gggctagaat tcgagcaatt atcttgttta ctaaaactga ataccttgct atctctttga
     4981 tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattctacc
     5041 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     5101 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     5161 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     5221 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     5281 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     5341 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     5401 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5461 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     5521 cctcatctcc gggcctttcg acctttaatt aaccaccatg gtgagcaagg gcgaagagaa
     5581 taacatggca atcatcaagg agttcatgcg cttcaaggtt cgcatggagg gctccgtgaa
     5641 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gctttcagac
     5701 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     5761 tcagttcacc tacggctcca aggcatacgt gaagcacccc gccgacatcc ccgactactt
     5821 caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     5881 cgtggtgacc gtgacccagg actcgtccct gcaagacggc gagttcatct acaaggtgaa
     5941 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaaaaaga ctatgggctg
     6001 ggaggcatcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat
     6061 gaggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca cctacaaggc
     6121 caagaagccc gtgcaactgc ccggcgccta catcgtcggc atcaagttgg acatcacctc
     6181 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     6241 cggcggcatg gacgagctat acaagtaggt ttaaacatca acctctggat tacaaaattt
     6301 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6361 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6421 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6481 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6541 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6601 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6661 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6721 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6781 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6841 tctccctttg ggccgcctcc ccgcctggcc tgcaggggta cctttaagac caatgactta
     6901 caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat
     6961 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     7021 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     7081 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     7141 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
     7201 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     7261 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
     7321 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
     7381 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7441 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7501 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7561 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7621 ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg
     7681 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7801 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7861 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8881 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8941 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9001 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9061 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9121 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9181 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9241 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9301 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9361 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9421 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9481 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9541 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9601 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9661 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9721 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9781 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9841 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc
     9901 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9961 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
    10021 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
    10081 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
    10141 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
