LOCUS       sequence-409295-        1251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dimerization dependent fluorescent protein RA anchored to cytosolic 
            face of the lysosomal membrane with human LAMP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Miner GE, Smith SY, Showalter WK, So CM, Ragusa JV, Powers AE, 
            Zanellati MC, Hsu CH, Marchan MF, Cohen S
  TITLE     Contact-FP: A Dimerization-Dependent Fluorescent Protein Toolkit for
            Visualizing Membrane Contact Site Dynamics.
  JOURNAL   Contact (Thousand Oaks). 2024 Feb 4;7:25152564241228911. doi: 
            10.1177/25152564241228911. eCollection 2024 Jan-Dec.
  PUBMED    38327561
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Contact 
            (Thousand Oaks)."; date: "2024-02-4"; volume: "7:25152564241228911. 
            doi"; pages: " 10.1177/25152564241228911. eCollection 2024 Jan-Dec"
COMMENT     Sequence Label: Lyso-RA
FEATURES             Location/Qualifiers
     source          1..1251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..84,85..1251)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
ORIGIN
        1 atggcggccc ccggcagcgc ccggcgaccc ctgctgctgc tactgctgtt gctgctgctc
       61 ggcctcatgc attgtgcgtc agcagcaatg tttatggtga aaaatggcaa cgggaccgcg
      121 tgcataatgg ccaacttctc tgctgccttc tcagtgaact acgacaccaa gagtggccct
      181 aagaacatga cctttgacct gccatcagat gccacagtgg tgctcaaccg cagctcctgt
      241 ggaaaagaga acacttctga ccccagtctc gtgattgctt ttggaagagg acatacactc
      301 actctcaatt tcacgagaaa tgcaacacgt tacagcgtcc agctcatgag ttttgtttat
      361 aacttgtcag acacacacct tttccccaat gcgagctcca aagaaatcaa gactgtggaa
      421 tctataactg acatcagggc agatatagat aaaaaataca gatgtgttag tggcacccag
      481 gtccacatga acaacgtgac cgtaacgctc catgatgcca ccatccaggc gtacctttcc
      541 aacagcagct tcagcagggg agagacacgc tgtgaacaag acaggccttc cccaaccaca
      601 gcgccccctg cgccacccag cccctcgccc tcacccgtgc ccaagagccc ctctgtggac
      661 aagtacaacg tgagcggcac caacgggacc tgcctgctgg ccagcatggg gctgcagctg
      721 aacctcacct atgagaggaa ggacaacacg acggtgacaa ggcttctcaa catcaacccc
      781 aacaagacct cggccagcgg gagctgcggc gcccacctgg tgactctgga gctgcacagc
      841 gagggcacca ccgtcctgct cttccagttc gggatgaatg caagttctag ccggtttttc
      901 ctacaaggaa tccagttgaa tacaattctt cctgacgcca gagaccccgc ctttaaagct
      961 gccaacggct ccctgcgagc gctgcaggcc acagtcggca attcctacaa gtgcaacgcg
     1021 gaggagcacg tccgtgtcac gaaggcgttt tcagtcaata tattcaaagt gtgggtccag
     1081 gctttcaagg tggaaggtgg ccagtttggc tctgtggagg agtgtctgct ggacgagaac
     1141 agcatgctga tccccatcgc tgtgggtggt gccctggcgg ggctggtcct catcgtcctc
     1201 atcgcctacc tcgtcggcag gaagaggagt cacgcaggct accagactat c
//
