LOCUS       sequence-409403-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with E2-Crimson reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPEW
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        5040..5539
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5075..5098)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5472..5491
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5557..6234
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(6295..6315)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6713..6724)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6914..7547
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7676..7692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7676..7692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7689..7711)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7700..7716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7724..7730,7731..7748,7749..7754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7769..7790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7907..7924)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8078..8666)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8158..8177)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8837..9628,9629..9697))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9460..9479
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9698..9802)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9850..9984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9887..9906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9941..9960
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10152,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgttccat gtccttatat ggactcatct ttgcctattg
     2221 cgacacacac tcaatgaaca cctactacgc gctgcaaaga gccccgcagg cctgaggtgc
     2281 ccccacctca ccactcttcc tatttttgtg taaaaatcca gcttcttgtc accacctcca
     2341 aggaggggga ggaggaggaa ggcaggttcc tctaggctga gccgaatgcc cctctgtggt
     2401 cccacgccac tgatcgctgc atgcccacca cctgggtaca cacagtctgt gattcccgga
     2461 gcagaacgga ccctgcccac ccggtcttgt gtgctactca gtggacagac ccaaggcaag
     2521 aaagggtgac aaggacaggg tcttcccagg ctggctttga gttcctagca ccgccccgcc
     2581 cccaatcctc tgtggcacat ggagtcttgg tccccagagt cccccagcgg cctccagatg
     2641 gtctgggagg gcagttcagc tgtggctgcg catagcagac atacaacgga cggtgggcct
     2701 agacccaggc tgtgtagacc cagccccccc gccccgcagt gactaggtca cccactaacg
     2761 ccccaggcct ggtcttggct gggcgtgact gttaccctca aaagcaggca gctccagggt
     2821 aaaaggtgcc ctgccctgta gagcccacct tccttcccag ggctgcggct gggtaggttt
     2881 gtagccttca tcacgggcca cctccagcca ctggaccgct ggcccctgcc ctgtcctggg
     2941 gagtgtggtc ctgcgacttc taagtggccg caagccacct gactccccca acaccacact
     3001 ctacctctca agcccaggtc tctccctagt gacccaccca gcacatttag ctagctgagc
     3061 cccacagcca gaggtcctca ggccctgctt tcagggcagt tgctctgaag tcggcaaggg
     3121 ggagtgactg cctggccact ccatgccctc caagagctcc ttctgcagga gcgtacagaa
     3181 cccagggcct tggcacccgt gcagaccctg gcccacccca cctgggcgct cagtgcccaa
     3241 gagatgtcca caactaggat gtcccgcggt gggtgggggg ccagagagac gggcaggccg
     3301 ggggcaggcc tggccatgcg gggccgaacc gggcactgcc cagcgtgggg cgcgggggcc
     3361 acggcgcgcg cccccagccc cccggcccag caccccaagg cggccaacgc caaaactctc
     3421 cctcctcctc ttcctcaatc tcgctctcgc tctttttttt tttcgcaaaa ggaggggaga
     3481 gggggtaaaa aaatgctgca ctgtgcggcg aagccggtga gtgagcggcg cggggccaat
     3541 cagcgtgcgc cgttccgaaa gttgcctttt atggctcgaa cggccgcggc ggcgccctat
     3601 aaaacccagc ggcgcgacgc gccaccaccg ccgagaccgc gtccgccccg cgagcacaga
     3661 gcctcgcctt tgccgatccg ccgcccgtcc aggctcgcat ctctccttca cgcgcccgcc
     3721 gccttacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     3781 gtgcctcctg aactacgtcc gccgtctagg taagtttaga gctcaggtcg agaccgggcc
     3841 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     3901 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcag
     3961 cgcttcgact agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc
     4021 gttgcctgca catcttggaa acacttgctg ggattacttc gacttcttaa cccaacagaa
     4081 ggctcgagaa ggtatattgc tgttgacagg atccacatta acaactttaa gtccagcaat
     4141 ttgagttaag ggtgttgctc tcaatgattt cactaatggt tcaattttta atttcttttc
     4201 ttctggttca aaattcaagt ttcaagtgaa agtgtaatat gcacccattt ctttcaataa
     4261 atcttctaaa tagtccacta atgttttatt ttgtttttta caaaatcaag cagcctctgc
     4321 tatcaatata gaaccttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc
     4381 ataactttct tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc
     4441 tctacccatt catttcaatc cagttaaagt ttttacaatc ttaactccat atttttcctg
     4501 agcgattcta tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg
     4561 aatgctattt aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg
     4621 atttccatct aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc
     4681 atctgggtca ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat
     4741 ttttcatgca ggatcaaatt ctggatttgg atttacaaca tttttcaatg tttcatcttc
     4801 aaatgcacga tcttcaccct caataacgtt atatcctgat tcacgtaatc tttttggggt
     4861 aaatttagtt cctgttccat taactgcgct aaaaataggg ccctgcctcg gacttcaagg
     4921 ggctagaatt cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat
     4981 acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg
     5041 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5101 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5161 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5221 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5281 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5341 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5401 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5461 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5521 ctcatctccg ggcctttcga cctttaatta accaccatgg atagcactga gaacgtcatc
     5581 aagcccttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     5641 atcgagggcg tgggcgaggg caagccctac gagggcaccc agaccgccaa gctgcaagtg
     5701 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt cttctacggc
     5761 tccaaggcgt acatcaagca ccccgccgac atccccgact acctcaagca gtccttcccc
     5821 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     5881 caggactcat ccctgcaaga cggcaccctc atctaccacg tcaagttcat cggcgtgaac
     5941 ttcccctccg acggccccgt aatgcagaag aaaactcttg gctgggagcc ctccactgag
     6001 cgcaactacc cccgcgacgg cgtgctgaag ggcgagaacc acatggcgct gaagctgaag
     6061 ggcggcggcc actacctgtg tgagttcaag tccatctaca tggctaagaa gcccgtgaag
     6121 ctgcccggct accactacgt ggactacaag ctcgacatca cctcccacaa cgaggactac
     6181 accgtggtgg agcagtacga acgcgccgag gcccgccacc acctgttcca gtaggtttaa
     6241 acatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6301 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6361 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6421 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6481 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6541 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6601 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6661 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6721 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6781 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcctgca
     6841 ggggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6901 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     6961 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     7021 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     7081 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     7141 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     7201 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     7261 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     7321 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7561 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7621 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7681 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7741 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7801 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7861 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7921 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7981 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8041 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8101 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8161 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8221 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8281 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8341 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8401 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8461 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8521 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8581 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8641 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8701 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8761 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8821 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8881 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8941 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9001 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9061 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9121 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9181 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9241 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9301 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9361 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9421 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9481 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9541 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9601 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9661 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9721 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9781 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9841 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9901 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9961 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    10021 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    10081 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10141 aacttagtag tt
//
