LOCUS       sequence-409586-        9418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of IRF9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Cheon H, Holvey-Bates EG, Schoggins JW, Forster S, Hertzog P, 
            Imanaka N, Rice CM, Jackson MW, Junk DJ, Stark GR
  TITLE     IFNbeta-dependent increases in STAT1, STAT2, and IRF9 mediate 
            resistance to viruses and DNA damage.
  JOURNAL   EMBO J. 2013 Oct 16;32(20):2751-63. doi: 10.1038/emboj.2013.203. 
            Epub 2013 Sep 24.
  PUBMED    24065129
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.203"; journalName: "EMBO J"; date: "2013-10-16- 
            16"; volume: "32"; issue: "20"; pages: "2751-63"
COMMENT     Sequence Label: pLV-IRF9
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(28..46,47..69,70..88,89..111,112..130,131..153,
                     154..172,173..195,196..214,215..237,238..256,257..279,
                     280..298)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     enhancer        339..642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        643..846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     797..817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     843..867
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     917..933
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     917..933
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             1222..1230
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2120..2143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        2218..2575
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2218..2409
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2241..2261)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2426..2561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2585..3184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2585..2604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3081..3101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3240..3256)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3241..3257)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3259..3847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3312..3332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3730..3741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3976..4009)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4035..4215
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4287..4408
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4324..4343)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4378..4397
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4622..4641)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4623..4641)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4651..4668)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4651..4667)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4660..4682)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4809..5264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4896..4915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5106..5127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5290..5394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5395..5463,5464..6255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5613..5632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6426..7014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6915..6934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7168..7185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7302..7323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7338..7343,7344..7361,7362..7368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7376..7392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7381..7403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7400..7416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7400..7416
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7435..7455
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7437..7455
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7483..7709
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7710..7890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7937..8062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8555..8788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8973..9017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9284..9400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 actccttcga tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
       61 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
      121 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
      181 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
      241 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
      301 agtgaaagtc gagctcggta cccgggtcga gagttccgcg ttacataact tacggtaaat
      361 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      421 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      481 actgcccact tggcagtaca tcaagtgtgt catatgccaa gtacgccccc tattgacgtc
      541 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      601 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      661 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      721 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      781 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      841 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
      901 catagaagac accgactcta gaactagtgg atcccccagg atggcatcag gcagggcacg
      961 ctgcacccga aaactccgga actgggtggt ggagcaagtg gagagtgggc agtttcccgg
     1021 agtgtgctgg gatgatacag ctaagaccat gttccggatt ccctggaaac atgcaggcaa
     1081 gcaggacttc cgggaggacc aggatgctgc cttcttcaag gcctgggcaa tatttaaggg
     1141 aaagtataag gagggggaca caggaggtcc agctgtctgg aagactcgcc tgcgctgtgc
     1201 actcaacaag agttctgaat ttaaggaggt tcctgagagg ggccgcatgg atgttgctga
     1261 gccctacaag gtgtatcagt tgctgccacc aggaatcgtc tctggccagc cagggactca
     1321 gaaagtacca tcaaagcgac agcacagttc tgtgtcctct gagaggaagg aggaagagga
     1381 tgccatgcag aactgcacac tcagtccctc tgtgctccag gactccctca ataatgagga
     1441 ggagggggcc agtgggggag cagtccattc agacattggg agcagcagca gcagcagcag
     1501 ccctgagcca caggaagtta cagacacaac tgaggccccc tttcaagggg atcagaggtc
     1561 cctggagttt ctgcttcctc cagagccaga ctactcactg ctgctcacct tcatctacaa
     1621 cgggcgcgtg gtgggcgagg cccaggtgca aagcctggat tgccgccttg tggctgagcc
     1681 ctcaggctct gagagcagca tggagcaggt gctgttcccc aagcctggcc cactggagcc
     1741 cacgcagcgc ctgctgagcc agcttgagag gggcatccta gtggccagca acccccgagg
     1801 cctcttcgtg cagcgccttt gccccatccc catctcctgg aatgcacccc aggctccacc
     1861 tgggccaggc ccgcatctgc tgcccagcaa cgagtgcgtg gagctcttca gaaccgccta
     1921 cttctgcaga gacttggtca ggtactttca gggcctgggc cccccaccga agttccaggt
     1981 aacactgaat ttctgggaag agagccatgg ctccagccat actccacaga atcttatcac
     2041 agtgaagatg gagcaggcct ttgcccgata cttgctggag cagactccag agcagcaggc
     2101 agccattctg tccctggtgg actacaagga cgacgatgac aagtaggggc tgcaggaatt
     2161 cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg
     2221 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2341 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2401 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2461 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2521 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     2581 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     2641 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     2701 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     2761 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     2821 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     2881 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     2941 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3001 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3061 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3121 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3181 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     3241 gataccgtcg acctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3301 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3361 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3421 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3481 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3541 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3601 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     3661 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3721 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3781 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3841 tccccgcctg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     3901 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccac
     3961 gaagacaaga attgaataac ttcgtatagc atacattata cgaagttata ccgattctgc
     4021 tttttgcttc tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4081 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4141 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt cagtcagtgt
     4201 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4261 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4321 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4381 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4501 aatttttttt atttatgcag aggccgaggc cgcctcatcg atgcggccgc gcctcggcct
     4561 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     4621 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     4681 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4741 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4801 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5041 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5221 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     5281 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5341 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5401 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5461 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5521 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5581 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5641 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5701 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5761 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5821 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5881 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5941 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     6001 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6061 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7381 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     7441 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     7501 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     7561 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     7621 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     7681 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     7741 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7861 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7921 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7981 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     8041 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8101 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8161 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8221 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8281 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8341 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     8761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     8821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     8881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     9001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9121 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9181 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     9241 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
     9301 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9361 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgattatc cagccctc
//
