LOCUS       sequence-409767-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1884..1900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1884..1900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1897..1919)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2072..2660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2152..2171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2777..2801)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2901..3692,3693..3761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3524..3543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3762..3866)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3965..3987
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3979..3996
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3980..3996
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4620..4645,1..8))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaagttatga tatcaaaaat gattacgcca agctgtaagt ttaaacatga tcttactaac
       61 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
      121 atacgctaat gccaacctag tcatcagtgt acatcagaaa aacgtaggat tcgacgcttg
      181 ttgtgacaca cacttgcctt ctttccatta aaccccatct atccacttcc gatggcccct
      241 tccggcatga taagaagtga gtggctacat aagacttcac acctccttcg gagattcatc
      301 aatccaacta ctccgaattt tttacatttt acttctacat atattggaat tcgttccatc
      361 aacacattcc tgtaaaaaat atataagaat tgttactgtt tgcgggataa atgcccctca
      421 tatgagatga aaaagagacc aaaaatgggg cccgtttcaa agggagaggc cgttataaaa
      481 gaatttatgc gtttcaaggt ccacatggag ggatccatga acggacacga gttcgagatc
      541 gagggagagg gagagggacg tccatacgag ggaacccaaa ccgccaaggt gagttttatt
      601 atcgattttc gggattttct acttgtaatt accttttgtt tttaataagt tgcttttttc
      661 cagctcaagg tcaccaaggg aggaccactc ccattctcct gggacatcct ctccccacaa
      721 ttcatgtacg gatcccgtgc cttcaccaag cacccagccg acatcccaga ctactacaag
      781 caatccttcc cagagggatt caagtgggag cgtgtcatga acttcgagga cggaggagcc
      841 gtcaccgtca cccaaggtag gttttcagtt tcaatttttg ttgcaactac ctcaactcta
      901 tgttttcaga cacctccctc gaggacggaa ccctcatcta caaggtcaag ctccgtggaa
      961 ccaacttccc accagacgga ccagtcatgc aaaagaagac catgggatgg gaggcctcca
     1021 ccgagcgact ctacccagag gacggagtcc tcaagggaga catcaaggta aatttttcaa
     1081 gcttctaaat tccaaaataa accattttaa tttcttaatt ccagatggcc ctccggctca
     1141 aggacggagg acgttacctc gccgacttca agaccaccta caaggccaag aagccagtcc
     1201 aaatgccagg agcctacaac gtcgaccgta agctcgacat cacctcccac aacgaggact
     1261 acaccgtcgt cgagcaatac gagcgttccg agggacgtca ctccaccgga ggaatggacg
     1321 agctctacaa gcccgggtga tggtctctga gctatgcaaa atcctttcaa gcattccctt
     1381 cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt atttgctttt
     1441 ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct gagtgaagtg
     1501 aatgctatca aaatgtgatt ctgatatcca ggaaacggag aatctgtgcg gcaatgtctt
     1561 atcatttgaa acgcgccgta tcgatgctga catcatgcgg ctcccgtgtt agtcgagaag
     1621 atgggtacgg gcctttattc atccaacact ctacggcttc ttcaagagct agtagggcac
     1681 cctgtagttg gaaagggatt atttcgtatg gcgagcccat accgtctttc ttgcggaagc
     1741 ttaacacgat aggaagttgg atagtttcga acgatggttc catacggaac gctgtctcaa
     1801 ggatgagtct gacggagtgt aactcgaagg ctaggtggag gctcagtgat gataagtctg
     1861 cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1921 agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg tggagttcag
     1981 ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag caaaaggcca
     2041 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2101 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2161 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2221 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2281 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2341 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2401 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2461 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2521 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2581 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2641 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctctat
     2701 tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc aaatcgtcct
     2761 cgtgatacca attcggagcc tgcttttttg tacaaacttg ttgataatgg caattcaagg
     2821 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2881 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2941 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3001 gagggcttac catctggccc cagtgctgca atgataccgc gagagccacg ctcaccggct
     3061 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3121 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3181 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3241 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3301 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3361 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3421 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3481 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3541 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3601 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3661 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3721 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccaga tacctgaaac
     3901 aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca agcgccaggg
     3961 ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc acgcatctgg
     4021 aataaggaag tgccattccg cctgacctaa gtgcttttcg agatcgccaa accggagaac
     4081 gatcaggaga atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct
     4141 ccaaactttt tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc
     4201 ccaatccatc atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt
     4261 tttgactttg aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat
     4321 gagttccctc aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa
     4381 agactaatct ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac
     4441 agcttctatt tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat
     4501 gcataaatct tcattgttga gtttatcttg ttgatttttg aataaattat caactcttta
     4561 ctttttaatg ggttatgaaa taaataaaca ttgaaaactg ataaacaacg ttcatctcta
     4621 taacttcgta tagcatacat tatac
//
