LOCUS       sequence-409804-       11042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of GFP-KIF2c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11042)
  AUTHORS   Hong C, Schubert M, Tijhuis AE, Requesens M, Roorda M, van den Brink
            A, Ruiz LA, Bakker PL, van der Sluis T, Pieters W, Chen M, Wardenaar
            R, van der Vegt B, Spierings DCJ, de Bruyn M, van Vugt MATM, Foijer 
            F
  TITLE     cGAS-STING drives the IL-6-dependent survival of chromosomally 
            instable cancers.
  JOURNAL   Nature. 2022 Jul;607(7918):366-373. doi: 10.1038/s41586-022-04847-2.
            Epub 2022 Jun 15.
  PUBMED    35705809
REFERENCE   2  (bases 1 to 11042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04847-2"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7918"; pages: "366-373"
COMMENT     Sequence Label: pLV-CMV-KIF2C
FEATURES             Location/Qualifiers
     source          1..11042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2214..2593
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2594..2797
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2748..2768
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2842..2861
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2842..2860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2892..2894,2895..2897,2898..3608)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2892..2894,2895..2897,2898..3608)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2937..2958)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3198..3217)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3545..3566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5874..6373
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5909..5932)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6306..6325
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6394..6993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6394..6413)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6890..6910
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7010..7598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7063..7083)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7481..7492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7805..8438
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8566..8582)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8566..8582)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8579..8601)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8590..8606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8614..8620,8621..8638,8639..8644))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8659..8680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8797..8814)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8968..9556)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9048..9067)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9727..10518,10519..10587))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10350..10369
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10588..10692)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10740..10874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10777..10796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10831..10850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11042,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagcgc gttgacattg
     2221 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2281 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2341 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2401 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2461 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2521 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2581 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2641 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2701 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2761 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     2821 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg
     2881 agctcggatc catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2941 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3001 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3061 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3121 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3181 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3241 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3301 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3361 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3421 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3481 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3541 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3601 tgtacaagta ctcagatctc gagctcaagc ttcgaattcg gcacgagctc cgaatggcca
     3661 tggactcgtc gcttcaggcc cgcctgtttc ccggtctcgc tatcaagatc caacgcagta
     3721 atggtttaat tcacagtgcc aatgtaagga ctgtgaactt ggagaaatcc tgtgtttcag
     3781 tggaatgggc agaaggaggt gccacaaagg gcaaagagat tgattttgat gatgtggctg
     3841 caataaaccc agaactctta cagcttcttc ccttacatcc gaaggacaat ctgcccttgc
     3901 aggaaaatgt aacaatccag aaacaaaaac ggagatccgt caactccaaa attcctgctc
     3961 caaaagaaag tcttcgaagc cgctccactc gcatgtccac tgtctcagag cttcgcatca
     4021 cggctcagga gaatgacatg gaggtggagc tgcctgcagc tgcaaactcc cgcaagcagt
     4081 tttcagttcc tcctgccccc actaggcctt cctgccctgc agtggctgaa ataccattga
     4141 ggatggtcag cgaggagatg gaagagcaag tccattccat ccgaggcagc tcttctgcaa
     4201 accctgtgaa ctcagttcgg aggaaatcat gtcttgtgaa ggaagtggaa aaaatgaaga
     4261 acaagcgaga agagaagaag gcccagaact ctgaaatgag aatgaagaga gctcaggagt
     4321 atgacagtag ttttccaaac tgggaatttg cccgaatgat taaagaattt cgggctactt
     4381 tggaatgtca tccacttact atgactgatc ctatcgaaga gcacagaata tgtgtctgtg
     4441 ttaggaaacg cccactgaat aagcaagaat tggccaagaa agaaattgat gtgatttcca
     4501 ttcctagcaa gtgtctcctc ttggtacatg aacccaagtt gaaagtggac ttaacaaagt
     4561 atctggagaa ccaagcattc tgctttgact ttgcatttga tgaaacagct tcgaatgaag
     4621 ttgtctacag gttcacagca aggccactgg tacagacaat ctttgaaggt ggaaaagcaa
     4681 cttgttttgc atatggccag acaggaagtg gcaagacaca tactatgggc ggagacctct
     4741 ctgggaaagc ccagaatgca tccaaaggga tctatgccat ggcctcccgg gacgtcttcc
     4801 tcctgaagaa tcaaccctgc taccggaagt tgggcctgga agtctatgtg acattcttcg
     4861 agatctacaa tgggaagctg tttgacctgc tcaacaagaa ggccaagctg cgcgtgctgg
     4921 aggacggcaa gcaacaggtg caagtggtgg ggctgcagga gcatctggtt aactctgctg
     4981 atgatgtcat caagatgatc gacatgggca gcgcctgcag aacctctggg cagacatttg
     5041 ccaactccaa ttcctcccgc tcccacgcgt gcttccaaat tattcttcga gctaaaggga
     5101 gaatgcatgg caagttctct ttggtagatc tggcagggaa tgagcgaggc gcggacactt
     5161 ccagtgctga ccggcagacc cgcatggagg gcgcagaaat caacaagagt ctcttagccc
     5221 tgaaggagtg catcagggcc ctgggacaga acaaggctca caccccgttc cgtgagagca
     5281 agctgacaca ggtgctgagg gactccttca ttggggagaa ctctaggact tgcatgattg
     5341 ccacgatctc accaggcata agctcctgtg aatatacttt aaacaccctg agatatgcag
     5401 acagggtcaa ggagctgagc ccccacagtg ggcccagtgg agagcagttg attcaaatgg
     5461 aaacagaaga gatggaagcc tgctctaacg gggcgctgat tccaggcaat ttatccaagg
     5521 aagaggagga actgtcttcc cagatgtcca gctttaacga agccatgact cagatcaggg
     5581 agctggagga gaaggctatg gaagagctca aggagatcat acagcaagga ccagactggc
     5641 ttgagctctc tgagatgacc gagcagccag actatgacct ggagaccttt gtgaacaaag
     5701 cggaatctgc tctggcccag caagccaagc atttctcagc cctgcgagat gtcatcaagg
     5761 ccttgcgcct ggccatgcag ctggaagagc aggctagcag acaaataagc agcaagaaac
     5821 ggccccagtg agcggccgcg ccggctctag atcgcgaacg cgtgaattct accgggtagg
     5881 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     5941 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     6001 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     6061 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     6121 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6181 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6241 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6301 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6361 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     6421 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6481 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6541 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     6601 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     6661 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     6721 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     6781 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     6841 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     6901 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     6961 acctggtgca tgacccgcaa gcccggtgcc tgacgggcgc gtctggaaca atcaacctct
     7021 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7081 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7141 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7201 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7261 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7321 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7381 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7441 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7501 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7561 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     7621 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     7681 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     7741 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     7801 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     7861 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     7921 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     7981 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     8041 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     8101 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     8161 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     8221 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     8281 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8341 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8401 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     8461 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     8521 ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8581 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8641 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8701 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8761 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8821 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8881 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8941 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     9001 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9061 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9121 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9181 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9241 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9301 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9361 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9421 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9481 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9541 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9601 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9661 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9721 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9781 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9841 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9901 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9961 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    10021 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10081 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10141 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10201 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10261 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10321 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10381 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10441 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10501 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10561 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10621 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10681 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca
    10741 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10801 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10861 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
    10921 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
    10981 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
    11041 tt
//
