LOCUS       sequence-409846-        8386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ddGFP-A and ddGFP-B3-inserted Pcdh-alpha-4 lacking an intracellular 
            domain (ICD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T
  TITLE     Visualization of trans-interactions of a protocadherin-alpha between
            processes originating from single neurons.
  JOURNAL   iScience. 2023 Jul 17;26(7):107238. doi: 10.1016/j.isci.2023.107238.
            eCollection 2023 Jul 21.
  PUBMED    37534169
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-07-17"; volume: "26(7):107238. doi"; pages: " 
            10.1016/j.isci.2023.107238. eCollection 2023 Jul 21"
COMMENT     Sequence Label: pCX:cIPAD-3 (Delta-ICD)
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1733..1742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5375..5401
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5408..5427)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5473..5528
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5527..5546)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5889..5905)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5889..5905)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5902..5924)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5913..5929
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5937..5943,5944..5961,5962..5967))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5982..6003
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6062..6257
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6108..6243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6263..6397
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6300..6319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6354..6373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6465..6482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6636..7224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6716..6735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7395..8186,8187..8255))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8018..8037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8256..8360)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggggc gcsggggggg gggggggggg ggggggggsg
      541 gggsggggsg rggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc
     1261 ggggccgcck cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 ggaattttcc tggggaagtg gccaggaatc ccagcgcttg cttctttctt ttctgcttct
     1801 tgcaatctgg gaggcaggga acagcgctag cgcaaccatc aaagaattta tgaggttcaa
     1861 agtacgcatg gagggtagta tgaacgggca tgagttcgaa attgaaggag aaggggaagg
     1921 ccgcccctat gaggggaccc aaaccgccaa acttaaagtg acaaagggag gtccacttcc
     1981 ttttgcctgg gatatattga gcccacagtt catgtacgga agcgaagcct atgtacgaca
     2041 tcctgcagac ataccagatt acaagaagct gccctttcca gaaggcttta agtgggaaag
     2101 agtgatgaac tttgaagatg gcggcttggt aacagtgact caggacagca gtctccaaga
     2161 tggaactttg atctgtaagg tcaaaatgag gggtactaac tttccccccg atgggcccgt
     2221 catgcaaaaa aagacaatgg gctgggaagc ctcaaccgag atgttgtacc cagaagatgg
     2281 agtgctcaag ggacattctt atcaggcatt gaagctgaaa gatggcggac attacttggt
     2341 tgagtttgag actatctata tggccaagaa gcccgtacaa cttcctggag attactgcgt
     2401 agacaccaaa ctggatataa cctctcataa cgaggattac accattgtcg agcagtacgg
     2461 ccgcagtgaa ggtagacata gacttggaat ggatgagcga tataaggcta gccagatcca
     2521 ctactccatc cctgaggagg ccaaacacgg caccttcgtg ggccgcatcg cgcaggacct
     2581 ggggctggag ctgacggagc tggtgccccg cctgttcaga gtggcgtcca aggaccgcgg
     2641 agaccttctg gaggtaaatc tgcagaatgg cattttgttt gtgaactctc ggatcgaccg
     2701 ggaggagctg tgcgggcgga gcgcggagtg cagcatccac ctggaggtga tcgtggacag
     2761 gccgttgcag gttttccacg tggaggtgga ggtgagggac attaacgaca accctcccag
     2821 gttcccaaca acacaaaaga atctgttcat tgcagaatca aggccacttg acacttggtt
     2881 tccactagag ggcgcttcag acgcagatat cggaatcaat gctgtactga cttacagact
     2941 gagtccaaat gattactttt ctttggaaaa accatccaac gacgaacggg taaaaggtct
     3001 tggacttgta ttacggaaat ctttagaccg ggaggaaact ccagagataa ttttagtgct
     3061 tactgtcacg gacggaggaa agccagagct gaccggcagt gttcagttac tcatcactgt
     3121 gctggatgcc aatgataatg ctccagtttt tgacagatcc ctgtataccg tgaaattacc
     3181 agaaaacgtt ccaaatggga cattggtagt caaagtcaat gcctcagatt tagacgaagg
     3241 ggtaaatggg gatattatgt actcattttc tacagatatt tcaccaaatg tgaaatacaa
     3301 attccacata gaccctgtta gcggagagat tattgtaaag ggatacattg attttgaaga
     3361 atgcaaatcc tatgaaattc tcatagaggg aattgacaag ggacaacttc cactctctgg
     3421 gcactgtaaa gtcattgtac aagttgaaga catcaacgat aatgttccag aattggaatt
     3481 taaatctcta tcacttccaa tacgagagaa ctctccagtg ggcactgtca tcgcactcat
     3541 tagtgtgtct gatcgggaca cgggtgtcaa cgggcaggtg acctgctccc tgacaagtca
     3601 tgctagcgtt agtaagagcg aggaggtaat caaggagttt atgaggttta aggtcaggct
     3661 cgagggcagc atgaatgggc atgagtttga gatagagggt gaaggggaag gcagaccata
     3721 tgagggtact caaacagcca agctcaaagt aaccaagggc ggtccactcc ctttcgcatg
     3781 ggatattttg agtcccctta ttatgtatgg ttccaaaatg tatgtgaagc accctgcaga
     3841 tgtgcctgat tatatgaaac tgtcattccc tgagggtttc aagtgggaaa gagtgatgca
     3901 ttttgaagat ggtggactcg tcacagctac acaggacagt tccttgcaag atggtacact
     3961 tatttacaaa gtaaagatgc ggggtacaaa cttccctcca gatggtcccg ttatgcagaa
     4021 gaagacactc ggttgggact acgcaacaga gcgactctat cccgaggagg gcgttctcaa
     4081 aggtgaactg ctgggacgac tgaaactcaa ggacggcgga ctgaatctgg tggaaagtaa
     4141 gaccatatac atggctaaaa aacccgtaca actcccagga tactattttg tcgacacaaa
     4201 gctggatatt acctcccata atgaggatta caccattgta gagcaatacg agaggagtga
     4261 aggccggcat catctgggac atggtacagg ctctactgga tctggcagta gcgctagcgt
     4321 ccccttcaag ttggtgtcca cattcaagaa ttactattcg ctcgtgctgg acagcgccct
     4381 ggacagagag acaacagcgg actataaggt ggtggtgaca gcgcgggatg ggggctctcc
     4441 ctcgctgtgg gccacggcta gtgtgtctgt tgaggttgct gacgtgaacg acaatgcacc
     4501 tgtgttcgcg cagcccgaat acacggtgtt cgtgaaggag aacaacccgc ctggtgcgca
     4561 catcttcacg gtgtcagcga tggatgcgga cgcacaggag aacgcgctgg tgtcctactc
     4621 gctggtggag cggagggtgg gcgagcgctt gctgtcgagc tatgtgtctg tgcacgcgga
     4681 gagcggcaag gtgttcgcgc tgcagcctct ggaccatgag gagctggagc tgctgcggtt
     4741 ccaggtgagc gcgcgggatg ctggtgtacc tgccctgggc agcaatgtga ctctgcaggt
     4801 gtttgtgctg gacgagaatg acaacgcgcc cacactgctg gaacctgagg caggagtctc
     4861 tggtggaatc gtgagccggt tggtgtccag atcagtgggt gcaggccatg tggtggctaa
     4921 ggtgcgcgcg gtggatgcag actctggcta taatgcatgg ctctcttatg agctgcaatc
     4981 gtcagaaggc aattcccgta gccttttccg cgtaggtttg tatacgggcg agattagtac
     5041 tacgcgcata ctggatgaag cagattcgcc acgtcagcgc cttctggtgc tggtgaagga
     5101 ccatggtgac ccagcaatga ttgttaccgc cacagtgttg gtgtctctgg tagagaatgg
     5161 cccggtacca aaggctccat cgcgagtatc cacgagtgtc acacactctg aggcgtcact
     5221 ggtggatgtc aacgtgtacc tgatcattgc catctgtgca gtgtccagcc tgctagtgct
     5281 cacgctgctg ctgtacacag cgctgcgctg ttccactgtc cccagtgaga gcgtgtgcgg
     5341 gcctccaaaa ccggtaatgg tgtgctccag tgcatatccg tacgacgtac cggattatgc
     5401 gtgaagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5461 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     5521 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     5581 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     5641 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     5701 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     5761 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     5821 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     5881 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5941 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6001 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     6061 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6241 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     6301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6361 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     6421 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6481 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6541 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7201 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7261 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7321 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7381 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7441 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7501 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7561 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7621 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7681 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7741 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7801 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7861 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7921 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7981 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8041 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8101 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8161 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8221 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8281 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8341 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
