LOCUS       sequence-409883-       12690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal GFP 
            tag and an exofacial double HA tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12690)
  AUTHORS   Macabuag N, Dolphin AC
  TITLE     Alternative Splicing in Ca(V)2.2 Regulates Neuronal Trafficking via 
            Adaptor Protein Complex-1 Adaptor Protein Motifs.
  JOURNAL   J Neurosci. 2015 Oct 28;35(43):14636-52. doi: 
            10.1523/JNEUROSCI.3034-15.2015.
  PUBMED    26511252
REFERENCE   2  (bases 1 to 12690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3034-15"; journalName: "J Neurosci"; date: 
            "2015-10-28- 28"; volume: "35"; issue: "43"; pages: "14636-52"
COMMENT     Sequence Label: GFP Cav2.2 HA pCAGGS
FEATURES             Location/Qualifiers
     source          1..12690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1764..2477
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1764..2477
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1794..1822)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2417..2437
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4176..4202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4206..4232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9712..9731)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9777..9832
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9831..9850)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10193..10209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10193..10209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10206..10228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10217..10233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10241..10247,10248..10265,10266..10271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10286..10307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10366..10561
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10412..10547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10567..10701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10604..10623)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10658..10677
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10769..10786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10940..11528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11020..11039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11699..12490,12491..12559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12322..12341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12560..12664)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gcnncaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag
     1741 ggtaccgagc tcggatctaa aaaatgagta aaggagaaga acttttcact ggagttgtcc
     1801 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1861 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1921 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1981 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     2041 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     2101 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     2161 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     2221 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     2281 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     2341 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     2401 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     2461 tggatgaact atacaaaatg gtccgcttcg gggacgagct gggcggccgc tatgggggcg
     2521 cgggcggcgc ggagcgggcc cggggcggcg gggccggcgg ggctggcggc ccgggccccg
     2581 gggggctgcc gccgggccag cgggtcctct acaaacagtc gatcgcgcag cgcgcgcgga
     2641 ccatggcgct gtacaacccc atcccggtca agcagaactg cttcaccgtc aaccgctcgc
     2701 tcttcgtctt cagcgaggac aacgtcgtcc gcaaatacgc caagcgcatc accgagtggc
     2761 ctccgttcga gtacatgatc ctggccacca tcatcgccaa ctgcatcgtg ctggccctgg
     2821 agcagcacct ccctgacggg gacaagacgc ctatgtccga gcgcctggat gacaccgagc
     2881 cctacttcat tgggatcttc tgcttcgagg cggggatcaa gatcctggcc ctgggctttg
     2941 ttctgcacaa gggctcctat ctgcggaacg gctggaacgt catggacttc gtggtagtcc
     3001 tcacggggat tcttgccaca gccggaaccg acttcgacct gcgaacgctg agggctgtgc
     3061 gtgtgctaag gcccctgaag ctggtgtctg ggattccgag cttgcaggtg gtgctcaagt
     3121 ccatcatgaa ggccatggtc ccgctgctgc agatcgggct gctgctcttc ttcgccatcc
     3181 tcatgtttgc catcatcggc ctcgagttct acatgggcaa gttccacaag gcctgcttcc
     3241 ccaacagcac agacccagat cccgtgggtg acttcccctg tggcaaggag gccccggccc
     3301 ggctctgtga gggagacacg gagtgccggg agtactgggc cggacccaac tttggtatca
     3361 ccaactttga caacatcctg tttgccatct tgaccgtgtt ccagtgcatc accatggagg
     3421 gctggactga catcctctac aatacaaacg atgcagccgg caacacgtgg aactggctct
     3481 acttcatccc tctcatcatc atcggctcct tcttcatgct caacctggtg ctgggtgtgc
     3541 tctcaggaga gtttgctaag gagcgcgaga gagtggagaa ccgccgcgcc ttcctgaagc
     3601 tccggcggca gcagcagatt gagcgggagc tcaacgggta cctggagtgg atcttcaagg
     3661 ctgaggaagt catgctggct gaggaggaca ggaacgcaga ggagaagtcc cctctggatg
     3721 cagtgttgaa gagagcagct gccaagaaga gcagaagcga cctgatccag gccgaggagg
     3781 gggagggccg gctcacaggc ctctgtgctc ccggttcccc gttcgcccgt gccagcctga
     3841 aaagcgggaa gacggagagc tcgtcgtact tccggaggaa ggagaagatg ttccgcttct
     3901 tcatccggcg catggtgaag gcacagagct tctactggac ggtgctgtgt gtggtggcac
     3961 tgaacacact gtgcgtggcc atggtgcact acaaccagcc gcagcggctc accacggcat
     4021 tgtactttgc agagtttgtt ttcctgggtc tcttcctcac agagatgtcc ctgaagatgt
     4081 atggcctggg gcccaggagc tacttccggt cctccttcaa ctgttttgac tttggggtca
     4141 tcgtgggcag tatctttgaa gtggtctggg ctgcttaccc ctacgacgtc cccgactacg
     4201 ccggctaccc gtacgacgtc ccggactacg ccggcgtcga cgtcaagcca gggacctcct
     4261 tcgggatcag tgttctgcgg gctctgcgct tactgagaat cttcaaggtc accaaatact
     4321 ggaactccct acggaacctg gtggtgtctc tgctcaactc catgaagtcc atcatcagcc
     4381 tcctcttcct gctcttcctg ttcattgttg tctttgctct gctggggatg cagctgtttg
     4441 gaggccagtt caactttaaa gacgagaccc caacgaccaa ctttgacacc ttccctgctg
     4501 ctatcctcac cgtcttccag atcctgaccg gagaggactg gaatgcggtc atgtaccacg
     4561 ggatcgagtc gcaaggtggc gtcagcagag gaatgttctc ctccttctac ttcattgtcc
     4621 tcactctgtt tgggaactac accctgctga atgtcttcct ggccattgct gtggacaacc
     4681 ttgccaatgc tcaggagctg accaaggatg aagaggagat ggaagaagca gccaatcaga
     4741 aactcgcctt gcagaaggcc aaggaagtgg ctgaagtcag ccccatgtct gctgcgaaca
     4801 tctccattgc cgccaggcag cagaactcgg ccaaagcgcg ctcggtgtgg gagcagcgag
     4861 ccagccagct gcggctgcag aacctgaggg ccagctgcga ggcgctgtac agcgagatgg
     4921 accccgagga gaggctgcgc tacgccaccg cgcgccacct gcggcccgac gtgaagacgc
     4981 acctggaccg gccgctggtg gttgagcccg gccgcgacgc tccgcggggc cccccggggg
     5041 ggaagtcccg gccggatggc tcggaggccc ccgagggtgc ggatcccccg cgcaggcatc
     5101 accggcaccg agacaaggac aaggctcccg ccacggtccc ctcggcgggg gaacaggaca
     5161 gagccgaagc tctgagggca gagggcgggg agctggggcc ccgcgaggag cgggggcggc
     5221 cacgccgcag ccgcagcaag gaggccccgg gcgccccgga ggtgcgcagc gatcgcggcc
     5281 gtggcccgtg tcctgaaggt ggtcggcgcc accaccggcg cgggtcaccg gaggaggcag
     5341 ctgagaggga gccccgccgc caccgcgcgc accggcacgg gcccgacccg ggcaaggagg
     5401 gcccagcgtc cgggaccagg ggcgagcggc gcgcacgaca ccgcacgggc ccccgggcgt
     5461 gcccccggga ggctgagagc agcgaggagc ctgcgcggcg gcaccgggct cggcacaagg
     5521 cgccgcccac ccaggagacc gcggagaagg acaaggaggc tgccgagaag gggggcgagg
     5581 ccacggaggc cgagaaggac aaggaggctc ggaaccacca gcccaaggag ctgccctgtg
     5641 acctggaggc catcggcatg ctgggagtgg gcgctgtgca tacgctgccc agcacctgtc
     5701 tgcagaaggt ggaggagcag ccagaggatg cggacaacca gcggaacgtc acccgcatgg
     5761 gtagtcagcc cccggacacc agcaccactg tgcacattcc tgtgacgctg accggccccc
     5821 ccggggagac cacggtagtg cccagtggaa acgtggacct ggaaagtcaa gcagagggga
     5881 agaaggaggt ggaaaccagc gatgtgatga ggagcggccc tcggcccatt gtcccgtaca
     5941 gttccatgtt ctgtttaagc ccaaccaacc tgctgcgccg ctgctgccac tacattgtga
     6001 ccatgcgata cttcgagatg gtcatcctcg tggtcatcgc cctgagcagc atcgccctgg
     6061 ctgccgagga ccccgtgaga acagactctc ccaggaataa tgctctgaag tacatggact
     6121 acatcttcac aggcgtcttc accttcgaga tggtgataaa gatgatcgat ttgggactgc
     6181 tgctacaccc tggagcctat ttccgggatc tgtggaacat tctggacttc attgtggtca
     6241 gtggggccct ggtggcattt gctttctcag gatccaaagg gaaagacatc agcaccatca
     6301 aatccctgag agtcctgcgt gtgctgcggc cactcaagac catcaagcgg ctgcccaagc
     6361 tcaaggccgt gtttgactgt gtggtgaact cactgaaaaa cgtcctcaac atcctgatcg
     6421 tctacatgct cttcatgttc atctttgccg tcattgccgt gcagctcttc aaagggaagt
     6481 tcttctactg cacggacgaa tccaaggagc tggagcgtga ctgcaggggt cagtatttgg
     6541 attatgagaa ggaggaagtg gaagctcaac cgaggcagtg gaagaaatat gacttccact
     6601 atgacaacgt gctctgggct ctgctaacgc tcttcacggt gtccacaggg gaagggtggc
     6661 ccatggtgct gaaacactct gtggatgcca cctacgagga gcagggcccg agccccgggt
     6721 accgcatgga gctctccatc ttctacgtgg tctactttgt ggtctttccc ttcttctttg
     6781 tcaacatctt cgtggccttg atcatcatca ccttccagga gcagggggac aaggtgatgt
     6841 ccgagtgcag cctggagaag aacgagaggg cttgcatcga ctttgccatc agcgccagac
     6901 ccctgacgcg gtacatgccc cagaacaagc agtcgttcca gtataagacg tggacgttcg
     6961 tggtgtcccc accttttgag tacttcatca tggctatgat cgccctcaac actgtggtgc
     7021 tgatgatgaa gttctacgat gcgccgtacg agtatgagct aatgctgaaa tgcctgaaca
     7081 tcgtgttcac gtccatgttc tccatggagt gtgtgctgaa gatcatcgcc ttcggggtgc
     7141 tgaactattt cagagatgcc tggaatgtct ttgactttgt cacggtgttg ggaagtatta
     7201 ctgatatttt ggtaacagag attgcgaaca acttcatcaa cctcagcttc ctgcgcctgt
     7261 tccgggccgc gcggctcatc aagctgctgc gccagggcta caccatccgc atcctgcttt
     7321 ggaccttcgt gcagtccttc aaggcgctgc cctacgtgtg tctcctcatc gccatgctct
     7381 tcttcatcta cgccatcatt ggcatgcagg tttttgggaa catcgccctg gacgatgaca
     7441 ccagcatcaa caggcacaat aacttccgga cgtttctgca ggccttgatg ttgctgttca
     7501 ggagcgccac gggggaggcc tggcacgaga taatgctgtc gtgcctcagc agccgggcct
     7561 gcgacgagca ctccaatgcc agcgagtgcg gcagcgactt tgcctacttt tacttcgtct
     7621 ccttcatctt cctctgctcc tttctgatgt tgaacctctt tgtggctgtg atcatggaca
     7681 attttgagta ccttacacgg gactcttcca tcttagggcc tcaccacctg gatgagttca
     7741 tccgggtctg ggctgaatac gacccggccg cgtgtgggcg catcagttac agtgacatgt
     7801 ttgagatgct gaagcacatg tccccacccc tggggctggg gaagaagtgc cctgctcgag
     7861 tcgcgtacaa gcgcctggtc cgcatgaaca tgcccatctc cagtgaggac atgacggtgc
     7921 acttcacatc cacgctgatg gctctcattc ggacagcgct ggacatcaag ctggcaccag
     7981 ctgggaccaa gcagcaccag tgcgacgcag agctgaggaa ggagatttcc tgtgtgtggg
     8041 ccaatctgcc ccagaagacc ctggacttac tggtaccacc ccataagcct gacgagatga
     8101 cggtgggcaa agtctatgca gctctgatga tcttcgactt ctacaaacag aacaaaacca
     8161 gcagagacca gactcagcaa gcccccggag gcctgtccca gctgggcccc gtgtccctgt
     8221 tccacccgct gaaggccacg ctggagcaga ctcagccggc gctgcgtgga gcccgggctt
     8281 tcctgcggca gaagagttct gcctccctga gcaacggcgg ggccgtacaa acccaggaga
     8341 gcggcatcaa ggaatcggtt tcttggggca ctcagaggac ccaggatgtg ctttgcgagg
     8401 ccagggcacc cctggagcgg ggccactcag ctgagatccc agtcgggcag cctgggacac
     8461 tggctgtaga tgtccagatg cagaacatga ctctgagtgg ccctgacgcc gagccgcagc
     8521 cagggctgga gagccagggc cgagccgcct ccatgccccg cctggccgca gaaacacagc
     8581 ccgcgcctga cgccagcccc atgaagcgct ccatctccac gctggccccg cgcccccaca
     8641 cggcccggct cggcagcacc gcgctggacc gcccagcccc cagccaggcc ccgcaccatc
     8701 accaccaccg ctgccaccgc cgcagggaca ggaagcagcg gtccctggag aagggtccca
     8761 gcctgtctgc tgacacagat ggggcccccg acagcacggt ggggccagga ctgcccacgg
     8821 gggagggccc ccccggctgc aggcgggagc gggagcgcag gcaggagcgg ggccggtccc
     8881 aggagcggag gcagccctcg tcctcctcct cggagaagca tcgcttctac tcctgtgacc
     8941 gcttcggggg ccgcgaaccc ccacagccca agccctccct cagtagtcac cccacgtcgc
     9001 cgacggccgg gcaggagccg ggaccccacc cgcaggggag tggttcggtt cacgggagcc
     9061 ccctgctgtc cacatctggt gctagcaccc cgggccgggg gcggaggcag ctcccccaga
     9121 cccccttgac cccccgcccc agcgtcacct acaagacggc caactcctcg cctgtccact
     9181 tcgccggggc accgagcggc ctccccgcct tctcccccgg ccggctcagc cggggcctct
     9241 cagaacacaa cgccctgctg cagagggacc cgctcagccg gcccctggcg cccggctcgc
     9301 gcatcggctc cgacccctac ctggggcagc ggctggacag cgaggcgccc gcccgtgccc
     9361 tgcccgagga cgcgcccgcc tttgaggaga ccgcggccag caactcgggc cgctcctccc
     9421 ggacctcgta cgtgtcctca ctgacctcgc agcccccgcc gctgcgccgc gtgcccaacg
     9481 gctaccactg cactctgggg ctgggcgggg gcggccgggc gcggcgcggc tgccaccacc
     9541 ccgaccgcga ccgccggtgc tagactagtt ctagcaagct tcgacgaatt cgcatgcggc
     9601 cgctagctcg aggtaccgat atcgagctca attcactcct caggtgcagg ctgcctatca
     9661 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     9721 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     9781 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     9841 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     9901 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     9961 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
    10021 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
    10081 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
    10141 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
    10201 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
    10381 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    10441 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    10501 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    10561 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10621 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10681 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10741 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    11341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    11401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    11461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    11521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    11581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    11641 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    11701 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    11761 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11821 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11881 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11941 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12001 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12061 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12121 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12181 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12241 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12301 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    12361 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    12421 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    12481 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    12541 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    12601 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    12661 cgcgcacatt tccccgaaaa gtgccacctg
//
