LOCUS       sequence-409945-        6271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mesoplasma florum oriC plasmid expressing mNeonGreen fluorescent 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6271)
  TITLE     Fluorescent protein expression plasmids - Mesoplasma florum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMflT-o4-mNG
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3936..3956
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        3957..3975
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4855..4875
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        4876..4894
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(5002..5590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5082..5101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5596..5614
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     oriT            5672..5781
                     /note="incP origin of transfer"
ORIGIN
        1 acttttatta ttatgtgttt taatttattt tatgaagggg aattaatatg gaaacaaaag
       61 cgctttgaga aaaattaatt aataagttaa agaaggaaaa attaatagac caagacatta
      121 ttgaagaaca tattgttact tcagaattaa ttaaaatttc aaatacagag ttcgtaatcc
      181 ttgttagaag taatcttggt gtaactattt taaatgaatt taaagaagta tttgtgtacg
      241 aatttaaata agttttaaac agttatgtgt ctgttgattt tttaactaaa gaaatttttg
      301 agaaaaatac aaaaaaagag aataaaaaag aacccataaa tactgtttta tctgaaaatg
      361 cacttacttt tgagaatttt atagttgggt caagtaataa acaagctaat ttagctgcta
      421 aaaatgttgt tgcaaaccct ggtatgagtt ttaatccttt atttatttat ggagattcag
      481 gactgggtaa aacacattta ttacaagcta ttaaaaatca agcagaatta aatggaaaaa
      541 aagttttata tttaacatct gaagaattta caaaaagaat tgttaatgct ttaaacaaag
      601 gtgatttaag tgaaatagaa gaattaaaaa ctgagataaa ttctaatgaa ttctttattt
      661 tagatgatgt tcaattttta agtaaaaaag ataaaacaaa cgagtttttc tttaacatta
      721 ttaacaattt tactgaaaat ggtaaacaat tagttttttc tagtgataag actcctgaat
      781 tgttaaatgg ttttgataaa agaatgataa ctagatttaa ttcaggttta tcaacaccta
      841 ttaatgcttt agatattcca actgccaaat tgataattga agctgaaatt aaaaaacaag
      901 gtttaaaaca aaaaattaaa gaagatgccg ttgtttactt agctcaaaac tttagcgatg
      961 atgttagaaa aattaaaggt ttagttaata gattactttt ctttggtatt caaaacgatt
     1021 taggtcacat aattgatttg gaagatgtta ttgacttatt taaagataca ccttcagcta
     1081 atttaggatt attaaatgtt aaaaaaatta aagaagttgt tgctaaaaaa tatgatgtta
     1141 ctataaaagc catcgatggt aaagcaagaa caactgctat aaaaaatgct agacatcttt
     1201 ctatgtattt tgcaaaaata attttaaacc atacatcaac tcaaattggt gcagaatttg
     1261 gtggaagaga ccatagtaca gttttaagtg ctatttcgcg tattgaaaaa ttaatatata
     1321 aagaaaaaga attcaaaaaa atagttgagt ctttaaaaaa cgaaataata ggaaaataga
     1381 tgtaaattcg catctatttt ttttgtgtgg aaaagaaatt aaacttgttg aataaaaagt
     1441 catttaaacc tttatttacc gttgttttat gagtgttttc cacatatcaa ctgcattata
     1501 aatattactt atataaagaa taatataata tataaaagca tgcgtatttc ttttgttttt
     1561 attttataaa tatgagataa tatatattgt aaataagagg tgttatatga gtccctttag
     1621 tgagggttaa tgtcgtgact gggaaaaccc tggcggaatt cggccagtct acatgtactc
     1681 tttttgataa aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt
     1741 gactatttaa aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt
     1801 gatagcggga acaaataatt agatgtcctt ttttaggagg gcttagtttt ttgtacccag
     1861 tttaagaata cctttatcat gtgattctaa agtatccaga gaatatctgt atgctttgta
     1921 tacctatggt tatgcataaa aatcccggtg ataaaagtat ttatcactgg gatttttatg
     1981 cccttttggg tttttgaatg gaggaaatct agatgaaaat cataaatatc ggtgtattag
     2041 ctcacgttga tgcaggaaaa acaacattaa ctgaatcact tttatataac tctggtgcaa
     2101 ttactgaact tggttcagta gataaaggta ctactcgtac tgataataca ttattagaac
     2161 gtcaacgtgg aatcacaatt caaacaggta tcacatcttt tcaatgggaa aatacaaaag
     2221 taaatattat agatacacct ggacacatgg atttccttgc agaagtatac cgtagtcttt
     2281 cagtattaga tggtgctatt ttacttatca gcgctaaaga tggagttcaa gctcaaactc
     2341 gtatcttatt tcacgcatta cgtaaaatgg gtattccaac aattttcttt ataaacaaaa
     2401 ttgaccaaaa cggaattgat ttaagtacag tttatcaaga tatcaaagaa aaactttctg
     2461 ctgaaatcgt tattaaacaa aaagttgaat tatacccaaa cgtttgcgta acaaatttta
     2521 ctgaatcaga acaatgggat acagttatag aaggtaatga tgatttatta gaaaaataca
     2581 tgtcaggtaa atcattagaa gcattagaat tagaacaaga agaaagtatt cgtttccaaa
     2641 actgttcttt attcccttta taccatggaa gcgctaaaag taacataggt attgataact
     2701 taattgaagt tattactaac aaattttact cttcaactca tcgtggtcct tctgaattat
     2761 gcggtaacgt tttcaaaatt gaatatacaa aaaaacgtca acgtttagct tatatacgtc
     2821 tttatagtgg tgttttacat ttacgtgata gtgttcgtgt tagtgaaaaa gaaaagatta
     2881 aagttacaga aatgtatact tctattaacg gtgaattatg caaaattgac cgtgcatatt
     2941 caggtgaaat tgtaatttta caaaacgaat ttcttaaact taatagtgta cttggtgaca
     3001 caaaactttt accacaacgt aagaaaattg aaaatccaca cccattactt caaacaacag
     3061 tagaaccaag caaacctgaa caacgtgaaa tgcttttaga tgctctttta gaaattagtg
     3121 actctgaccc acttttacgt tactatgtag attctactac tcatgaaatt attctttctt
     3181 tccttggtaa agttcaaatg gaagttattt ctgcattatt acaagaaaaa tatcatgttg
     3241 aaatcgaatt aaaagaacct actgtaattt atatggaacg tccattaaaa aatgctgaat
     3301 atacaattca tattgaagtt ccaccaaatc cattttgggc ttctattggt ctttctgttt
     3361 ctccacttcc acttggtagc ggaatgcaat atgaaagtag cgtaagttta ggttatctta
     3421 atcaaagttt ccaaaacgca gttatggaag gtattcgtta cggttgcgaa caaggtttat
     3481 acggttggaa tgttacagac tgcaaaatct gttttaagta tggactttac tattcacctg
     3541 tatcaacacc tgctgacttt cgtatgcttg caccaattgt tttagaacaa gttttaaaga
     3601 aagctggaac tgaactttta gaaccatacc tttcttttaa aatctatgca ccacaagaat
     3661 acttaagtcg tgcttataac gatgcaccta aatactgtgc taatattgtt gatactcaat
     3721 taaagaacaa cgaagtaatt ttaagcggag aaattcctgc acgttgtatt caagaatatc
     3781 gtagtgattt aacatttttc actaatggac gttctgtttg cttaactgaa ttaaaaggtt
     3841 atcatgttac tactggtgaa cctgtatgcc aaccacgtcg tcctaatagt cgtattgata
     3901 aagttcgtta tatgttcaac aaaatcacat aataatacta gtagcggccg ctgcagtaat
     3961 acgactcact atagggcata cgatttaggt gacactatag tagggataac agggtaattt
     4021 aswwrrymgr gswryakaca agatcttttc gccaggcatg ctcataaaaa atttatttgc
     4081 tttcaggaaa ttttttctgt ataatagatt catacgactc actatactcg agaaggaggc
     4141 aacaagatgg tttctaaagg agaagaaaaa aatatggcaa gtttaccagc aacacatgag
     4201 ctacatatct ttggttcaat aaacggtgta gattttgata tggttggtca aggaacagga
     4261 aatcctaacg atggttacga agaattaaat ttaaaatcaa caaaaggtga tttacaattt
     4321 tctccatgga ttttagtacc tcacattgga tatggttttc atcaatactt accatatcct
     4381 gatggtatga gtcctttcca agctgctatg gttgatggtt ctgggtatca agttcataga
     4441 acaatgcaat tcgaagatgg agcatcttta actgttaatt acaggtatac gtacgaggga
     4501 tcacatatta aaggagaagc tcaagttaaa ggtactggtt ttcctgcaga tggtccagtt
     4561 atgactaatt ccttaactgc agcagattgg tgcagaagta aaaagacata ccctaatgat
     4621 aaaacaatta tttcaacttt taaatggtca tatactacag gtaacggaaa aagatataga
     4681 agtacagcaa gaacaactta tacttttgct aagccaatgg cagctaatta tttaaaaaat
     4741 caaccaatgt atgtttttag aaaaacagaa ttaaaasayw rtraawsmsa ayyrmrtykt
     4801 mmwgaatgkc rwawwgmtww wrytsrtkwt atgttcaaca aaatcacata ataatactag
     4861 tagcggccgc tgcagtaata cgactcacta tagggcatac gatttaggtg acactatagt
     4921 agggataaca gggtaattta gtaggtaggg ctatatggtc gttatgcgat actgatgcat
     4981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5161 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5401 agagttcttg aagttgtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5581 aggatctcaa cgcgcaatta accctcacta aagggaacaa aagctggagc gctgcaggaa
     5641 ttcgatatca agcttatcga taccgtcgac gccggccagc ctcgcagagc aggattcccg
     5701 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     5761 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     5821 accctttggc aaaatcctgt atatcgtgcg aaaaagcatg gatataccga aaaaatcgct
     5881 ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct ggtacccagc gatgaggatg
     5941 aacccactca gggacctgat gtgacgtgcc gtgaacacga gcgtgtttag tttttgatgg
     6001 ttgtcaagtt cttttcattg tattcaaacc cccttttaaa gttgataatt cttgttaatt
     6061 ataatataag tgctggaaaa agtatagttt tttattgtta tttttaagaa aatttatcca
     6121 ttgtgttttt atatgaactt tttcaacatt ttttataacc ttttgtggaa aagcggaaaa
     6181 aagatatata aatactaaac tattgctttt tagtgttatt cattatgttt atttcttttt
     6241 aaaatatttt tttatccaca cattgtggaa g
//
