LOCUS       sequence-410027-       11244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xflag fused mouse miniSOX in mammalian cells, for 
            lentivirus generation. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch
            R
  TITLE     Evaluation of the determinants for improved pluripotency induction 
            and maintenance by engineered SOX17.
  JOURNAL   Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597.
  PUBMED    37607832
REFERENCE   2  (bases 1 to 11244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597."
COMMENT     Sequence Label: pLVTHM-miniSOX-3xflag
FEATURES             Location/Qualifiers
     source          1..11244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2147..3325
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2378..3316
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3273..3293
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3373..3490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4417..4482
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4500..5088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4553..4573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4971..4982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5256..5274,5275..5297,5298..5316,5317..5339,5340..5358,
                     5359..5381,5382..5400,5401..5423,5424..5442,5443..5465,
                     5466..5484,5485..5507,5508..5526)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5555..5769
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5690..5709
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5807..5840)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5860..6040
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6065..6083)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6066..6083)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6373..6392)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6606..6625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6725..6747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6785..6803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6871..6975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6976..7044,7045..7836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7194..7213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8007..8595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8496..8515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8749..8766
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8836..8856)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8841..9170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9021..9156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10304..10369
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10499..10519
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10944..11078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10981..11000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11035..11054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcga tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa
     2101 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2161 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2221 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2281 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2341 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2401 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
     3361 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3481 tcaaaatttt atcgatcacg agactagcct cgaggtttaa acatgtctcg catccggcgg
     3541 ccgatgaacg cctttatggt gtgggccaaa gacgaacgca agcggttggc acagcagaac
     3601 ccagatctgc acaacgcaga gctaagcaag atgctaggca agtcttggaa ggcgttgacc
     3661 tttgcagaga agcggccctt caatgaagag gccgtgcggc tgcgcgtgca gcatatgcag
     3721 gaccacccca actacaagta ccggccgcgg cggcgcaagc aggtgaagcg catgaagcgg
     3781 gtggagggag gcttcctgca cgctctcgtc gagccccagg ccggcgcgct tggtcccgag
     3841 ggcggccgcg tggccatgga tggcctgggt ctgcctttcc cggagccggg ctatccggcc
     3901 ggtcctccgc tgatgtctcc gcacatgggc ccccactatc gggactgcca gggactgggc
     3961 gctcccgcgc tcgacggcta ccctctgccc actccggaca catccccgct ggatggcgtg
     4021 gagcaggacc cggctttctt tgcagccccg ctgccagggg actgcccggc ggccggcacc
     4081 tacacttacg ctccagtctc ggactatgca gtgtccgtag agccgcccgc tggccccatg
     4141 cgagtggggc cggacccctc gggccctgcg atgccgggga tcctggcgcc ccccagcgct
     4201 ctgcacctgt actacggcgc gatgggctcg cccgccgcaa gtgcggggcg cggtttccac
     4261 gcgcaacccc agcagccgct gcaaccgcag gcagagctcc taggggaggt ggaccgcacg
     4321 gaattcgaac agtatctgcc ctttgtgtat aagcccgaga tgggtcttcc ctaccaggga
     4381 cacgactgcg gagtgaacct cacgcgtggc agcggcgact acaaggacca cgacggcgac
     4441 tacaaggacc acgacatcga ctacaaggac gacgacgaca agtagactag tcatatgata
     4501 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4561 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4621 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4681 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4741 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4801 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4861 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     4921 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4981 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5041 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtacgtat
     5101 ggccaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5161 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
     5221 tcaccatgct agtggatccc cctcgagttt accactccct atcagtgata gagaaaagtg
     5281 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5341 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5401 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5461 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     5521 tagagaaaag tgaaagtcgg ggctgcagga attcgaacgc tgacgtcatc aacccgctcc
     5581 aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag
     5641 gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt
     5701 tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat aagttctgta
     5761 tgagaccacg cggcgtctcc aagcttatcg ataccgtcgc atgggaataa cttcgtatag
     5821 catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag
     5881 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5941 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6001 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     6061 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     6121 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     6181 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     6241 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     6301 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     6361 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     6421 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     6481 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     6541 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     6601 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     6661 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6721 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     6781 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6841 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6901 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6961 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7021 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7081 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7141 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7201 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7261 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7321 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7381 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7441 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7501 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7561 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7621 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7681 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7741 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7801 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7861 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     8821 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     8881 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     8941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9001 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9061 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9121 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     9181 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     9241 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     9301 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     9361 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     9421 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     9481 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     9541 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     9601 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     9661 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     9721 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     9781 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     9841 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     9901 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     9961 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    10021 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    10081 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
    10141 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
    10201 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
    10261 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    10321 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    10381 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
    10441 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
    10501 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
    10561 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
    10621 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
    10681 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
    10741 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
    10801 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    10861 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    10921 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    10981 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11041 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11101 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11161 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11221 aaatatgtac tacaaactta gtag
//
