LOCUS       sequence-410076-        3607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMSCV backbone with FLAG tag for protein expression and puromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3607)
  TITLE     Hyonchol Jang Lab Oct4 plasmids 2023
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMSCV-FLAG-puro-Mock
FEATURES             Location/Qualifiers
     source          1..3607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    670..686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(694..700,701..718,719..724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    739..760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(877..894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1048..1636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1128..1147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1807..2598,2599..2667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2430..2449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2668..2772)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2840..2858
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2896..2918)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3018..3037
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3231..3253
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3381..3400
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(3601..3607,1..508)
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
       61 tacataactg agaatagaga agttcagatc aaggttagga acagagagac agcagaatat
      121 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      181 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
      241 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      301 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      361 cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtgtatcc aataaaccct
      421 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      481 tgactacccg tcagcggggg tctttcatgg gtaacagttt cttgaagttg gagaacaaca
      541 ttctgagggt aggagtcgaa tattaagtaa tcctgactca attagccact gttttgaatc
      601 cacatactcc aatactcctg aaatagttca ttatggacag cgcagaaaga gctggggaga
      661 attgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      781 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      841 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      901 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      961 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1021 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1081 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1141 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1201 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1261 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1321 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1381 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1441 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1501 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1561 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1621 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1681 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1741 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1801 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1861 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1921 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1981 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2041 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2101 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2161 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2221 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2281 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2341 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2401 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2461 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2521 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2581 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2641 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2701 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2761 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2821 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2881 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2941 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3001 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3061 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     3121 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3181 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3241 acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     3301 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     3361 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     3421 gcgccggtga tgccggccac gatgcgtccg gcgtagaggc gattagtcca atttgttaaa
     3481 gacaggatat cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct
     3541 atagagtacg agccatagat aaaataaaag attttattta gtctccagaa aaagggggga
     3601 atgaaag
//
