LOCUS       sequence-410271-       13236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal GFP 
            tag and a D122A mutation that disrupts the interaction with ?2?-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Page KM, Heblich F, Davies A, Butcher AJ, Leroy J, Bertaso F, Pratt 
            WS, Dolphin AC
  TITLE     Dominant-negative calcium channel suppression by truncated 
            constructs involves a kinase implicated in the unfolded protein 
            response.
  JOURNAL   J Neurosci. 2004 Jun 9;24(23):5400-9. doi: 
            10.1523/JNEUROSCI.0553-04.2004.
  PUBMED    15190113
REFERENCE   2  (bases 1 to 13236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.0553-04"; journalName: "J Neurosci"; date: 
            "2004-06-9- 9"; volume: "24"; issue: "23"; pages: "5400-9"
COMMENT     Sequence Label: GFP Cav2.2 D122A pcDNA3
FEATURES             Location/Qualifiers
     source          1..13236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      117..545
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(204..223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     414..435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(554..574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        559..888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      739..874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     801..820
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             955..1749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1009..1028)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1619..1638
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1923..2044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1960..1979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2014..2033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2093..2109)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2093..2109)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2106..2128)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2117..2133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2141..2147,2148..2165,2166..2171))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2186..2207
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2324..2341)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2495..3083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2575..2594)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3254..4045,4046..4114))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3877..3896
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4115..4219)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4294..4313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4485..4864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4865..5068
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5019..5039
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5113..5132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5113..5131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5223..5936
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5223..5936
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5253..5281)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5876..5896
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(13039..13057)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(13040..13057)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(13077..13094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(13083..13236,1..71)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
       61 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
      121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      541 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
      841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
      901 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     1021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     1081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     1141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     1201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     1261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     1321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     1381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     1441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     1801 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1861 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1921 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2041 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     2101 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2161 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2221 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2281 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2341 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2401 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2461 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2521 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2581 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2641 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2701 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2761 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2821 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2881 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2941 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3001 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3061 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3121 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3181 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3241 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3301 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3361 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3421 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3481 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3541 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3601 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3661 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3721 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3781 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3841 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3901 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3961 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4021 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4081 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4141 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4201 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     4261 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4321 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     4381 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     4441 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     4501 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     4561 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     4621 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     4681 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     4741 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     4801 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4861 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4921 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4981 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     5041 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
     5101 gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat
     5161 ccactagtaa cggccgccag tgtgctggaa ttcctcgagg gtaccgagct cggatctaaa
     5221 aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     5281 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     5341 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     5401 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     5461 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     5521 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     5581 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     5641 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     5701 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     5761 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     5821 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     5881 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaatgg
     5941 tccgcttcgg ggacgagctg ggcggccgct atgggggcgc gggcggcgcg gagcgggccc
     6001 ggggcggcgg ggccggcggg gctggcggcc cgggccccgg ggggctgccg ccgggccagc
     6061 gggtcctcta caaacagtcg atcgcgcagc gcgcgcggac catggcgctg tacaacccca
     6121 tcccggtcaa gcagaactgc ttcaccgtca accgctcgct cttcgtcttc agcgaggaca
     6181 acgtcgtccg caaatacgcc aagcgcatca ccgagtggcc tccgttcgag tacatgatcc
     6241 tggccaccat catcgccaac tgcatcgtgc tggccctgga gcagcacctc cctgacgggg
     6301 ccaagacgcc tatgtccgag cgcctggatg acaccgagcc ctacttcatt gggatcttct
     6361 gcttcgaggc ggggatcaag atcctggccc tgggctttgt tctgcacaag ggctcctatc
     6421 tgcggaacgg ctggaacgtc atggacttcg tggtagtcct cacggggatt cttgccacag
     6481 ccggaaccga cttcgacctg cgaacgctga gggctgtgcg tgtgctaagg cccctgaagc
     6541 tggtgtctgg gattccgagc ttgcaagtgg tgctcaagtc catcatgaag gccatggtcc
     6601 cgctgctgca gatcgggctg ctgctcttct tcgccatcct catgtttgcc atcatcggcc
     6661 tcgagttcta catgggcaag ttccacaagg cctgcttccc caacagcaca gacccagatc
     6721 ccgtgggtga cttcccctgt ggcaaggagg ccccggcccg gctctgtgag ggagacacgg
     6781 agtgccggga gtactgggcc ggacccaact ttggtatcac caactttgac aacatcctgt
     6841 ttgccatctt gaccgtgttc cagtgcatca ccatggaggg ctggactgac atcctctaca
     6901 atacaaacga tgcagccggc aacacgtgga actggctcta cttcatccct ctcatcatca
     6961 tcggctcctt cttcatgctc aacctggtgc tgggtgtgct ctcaggagag tttgctaagg
     7021 agcgcgagag agtggagaac cgccgcgcct tcctgaagct ccggcggcag cagcagattg
     7081 agcgggagct caacgggtac ctggagtgga tcttcaaggc tgaggaagtc atgctggctg
     7141 aggaggacag gaacgcagag gagaagtccc ctctggatgc agtgttgaag agagcagctg
     7201 ccaagaagag cagaagcgac ctgatccagg ccgaggaggg ggagggccgg ctcacaggcc
     7261 tctgtgctcc cggttccccg ttcgcccgtg ccagcctgaa aagcgggaag acggagagct
     7321 cgtcgtactt ccggaggaag gagaagatgt tccgcttctt catccggcgc atggtgaagg
     7381 cacagagctt ctactggacg gtgctgtgtg tggtggcact gaacacactg tgcgtggcca
     7441 tggtgcacta caaccagccg cagcggctca ccacggcatt gtactttgca gagtttgttt
     7501 tcctgggtct cttcctcaca gagatgtccc tgaagatgta tggcctgggg cccaggagct
     7561 acttccggtc ctccttcaac tgttttgact ttggggtcat cgtgggcagt atctttgaag
     7621 tggtctgggc tgctgtcaag ccagggacct ccttcgggat cagtgttctg cgggctctgc
     7681 gcttactgag aatcttcaag gtcaccaaat actggaactc cctacggaac ctggtggtgt
     7741 ctctgctcaa ctccatgaag tccatcatca gcctcctctt cctgctcttc ctgttcattg
     7801 ttgtctttgc tctgctgggg atgcagctgt ttggaggcca gttcaacttt aaagacgaga
     7861 ccccaacgac caactttgac accttccctg ctgctatcct caccgtcttc cagatcctga
     7921 ccggagagga ctggaatgcg gtcatgtacc acgggatcga gtcgcaaggt ggcgtcagca
     7981 gaggaatgtt ctcctccttc tacttcattg tcctcactct gtttgggaac tacaccctgc
     8041 tgaatgtctt cctggccatt gctgtggaca accttgccaa tgctcaggag ctgaccaagg
     8101 atgaagagga gatggaagaa gcagccaatc agaaactcgc cttgcagaag gccaaggaag
     8161 tggctgaagt cagccccatg tctgctgcga acatctccat tgccgccagg cagcagaact
     8221 cggccaaagc gcgctcggtg tgggagcagc gagccagcca gctgcggctg cagaacctga
     8281 gggccagctg cgaggcgctg tacagcgaga tggaccccga ggagaggctg cgctacgcca
     8341 ccgcgcgcca cctgcggccc gacgtgaaga cgcacctgga ccggccgctg gtggttgagc
     8401 ccggccgcga cgctccgcgg ggccccccgg gggggaagtc ccggccggat ggctcggagg
     8461 cccccgaggg tgcggatccc ccgcgcaggc atcaccggca ccgagacaag gacaaggctc
     8521 ccgccacggt cccctcggcg ggggaacagg acagagccga agctctgagg gcagagggcg
     8581 gggagctggg gccccgcgag gagcgggggc ggccacgccg cagccgcagc aaggaggccc
     8641 cgggcgcccc ggaggtgcgc agcgatcgcg gccgtggccc gtgtcctgaa ggtggtcggc
     8701 gccaccaccg gcgcgggtca ccggaggagg cagctgagag ggagccccgc cgccaccgcg
     8761 cgcaccggca cgggcccgac ccgggcaagg agggcccagc gtccgggacc aggggcgagc
     8821 ggcgcgcacg acaccgcacg ggcccccggg cgtgcccccg ggaggctgag agcagcgagg
     8881 agcctgcgcg gcggcaccgg gctcggcaca aggcgccgcc cacccaggag accgcggaga
     8941 aggacaagga ggctgccgag aaggggggcg aggccacgga ggccgagaag gacaaggagg
     9001 ctcggaacca ccagcccaag gagctgccct gtgacctgga ggccatcggc atgctgggag
     9061 tgggcgctgt gcatacgctg cccagcacct gtctgcagaa ggtggaggag cagccagagg
     9121 atgcggacaa ccagcggaac gtcacccgca tgggtagtca gcccccggac accagcacca
     9181 ctgtgcacat tcctgtgacg ctgaccggcc cccccgggga gaccacggta gtgcccagtg
     9241 gaaacgtgga cctggaaagt caagcagagg ggaagaagga ggtggaaacc agcgatgtga
     9301 tgaggagcgg ccctcggccc attgtcccgt acagttccat gttctgttta agcccaacca
     9361 acctgctgcg ccgctgctgc cactacattg tgaccatgcg atacttcgag atggtcatcc
     9421 tcgtggtcat cgccctgagc agcatcgccc tggctgccga ggaccccgtg agaacagact
     9481 ctcccaggaa taatgctctg aagtacatgg actacatctt cacaggcgtc ttcaccttcg
     9541 agatggtgat aaagatgatc gatttgggac tgctgctaca ccctggagcc tatttccggg
     9601 atctgtggaa cattctggac ttcattgtgg tcagtggggc cctggtggca tttgctttct
     9661 caggatccaa agggaaagac atcagcacca tcaaatccct gagagtcctg cgtgtgctgc
     9721 ggccactcaa gaccatcaag cggctgccca agctcaaggc cgtgtttgac tgtgtggtga
     9781 actcactgaa aaacgtcctc aacatcctga tcgtctacat gctcttcatg ttcatctttg
     9841 ccgtcattgc cgtgcagctc ttcaaaggga agttcttcta ctgcacggac gaatccaagg
     9901 agctggagcg tgactgcagg ggtcagtatt tggattatga gaaggaggaa gtggaagctc
     9961 aaccgaggca gtggaagaaa tatgacttcc actatgacaa cgtgctctgg gctctgctaa
    10021 cgctcttcac ggtgtccaca ggggaagggt ggcccatggt gctgaaacac tctgtggatg
    10081 ccacctacga ggagcagggc ccgagccccg ggtaccgcat ggagctctcc atcttctacg
    10141 tggtctactt tgtggtcttt cccttcttct ttgtcaacat cttcgtggcc ttgatcatca
    10201 tcaccttcca ggagcagggg gacaaggtga tgtccgagtg cagcctggag aagaacgaga
    10261 gggcttgcat cgactttgcc atcagcgcca gacccctgac gcggtacatg ccccagaaca
    10321 agcagtcgtt ccagtataag acgtggacgt tcgtggtgtc cccacctttt gagtacttca
    10381 tcatggctat gatcgccctc aacactgtgg tgctgatgat gaagttctac gatgcgccgt
    10441 acgagtatga gctaatgctg aaatgcctga acatcgtgtt cacgtccatg ttctccatgg
    10501 agtgtgtgct gaagatcatc gccttcgggg tgctgaacta tttcagagat gcctggaatg
    10561 tctttgactt tgtcacggtg ttgggaagta ttactgatat tttggtaaca gagattgcga
    10621 acaacttcat caacctcagc ttcctgcgcc tgttccgggc cgcgcggctc atcaagctgc
    10681 tgcgccaggg ctacaccatc cgcatcctgc tttggacctt cgtgcagtcc ttcaaggcgc
    10741 tgccctacgt gtgtctcctc atcgccatgc tcttcttcat ctacgccatc attggcatgc
    10801 aggtttttgg gaacatcgcc ctggacgatg acaccagcat caacaggcac aataacttcc
    10861 ggacgtttct gcaggccttg atgttgctgt tcaggagcgc cacgggggag gcctggcacg
    10921 agataatgct gtcgtgcctc agcagccggg cctgcgacga gcactccaat gccagcgagt
    10981 gcggcagcga ctttgcctac ttttacttcg tctccttcat cttcctctgc tcctttctga
    11041 tgttgaacct ctttgtggct gtgatcatgg acaattttga gtaccttaca cgggactctt
    11101 ccatcttagg gcctcaccac ctggatgagt tcatccgggt ctgggctgaa tacgacccgg
    11161 ccgcgtgtgg gcgcatcagt tacagtgaca tgtttgagat gctgaagcac atgtccccac
    11221 ccctggggct ggggaagaag tgccctgctc gagtcgcgta caagcgcctg gtccgcatga
    11281 acatgcccat ctccagtgag gacatgacgg tgcacttcac atccacgctg atggctctca
    11341 ttcggacagc gctggacatc aagctggcac cagctgggac caagcagcac cagtgcgacg
    11401 cagagctgag gaaggagatt tcctgtgtgt gggccaatct gccccagaag accctggact
    11461 tactggtacc accccataag cctgacgaga tgacggtggg caaagtctat gcagctctga
    11521 tgatcttcga cttctacaaa cagaacaaaa ccagcagaga ccagactcag caagcccccg
    11581 gaggcctgtc ccagctgggc cccgtgtccc tgttccaccc gctgaaggcc acgctggagc
    11641 agactcagcc ggcgctgcgt ggagcccggg ctttcctgcg gcagaagagt tctgcctccc
    11701 tgagcaacgg cggggccgta caaacccagg agagcggcat caaggaatcg gtttcttggg
    11761 gcactcagag gacccaggat gtgctttgcg aggccagggc acccctggag cggggccact
    11821 cagctgagat cccagtcggg cagcctggga cactggctgt agatgtccag atgcagaaca
    11881 tgactctgag tggccctgac gccgagccgc agccagggct ggagagccag ggccgagccg
    11941 cctccatgcc ccgcctggcc gcagaaacac agcccgcgcc tgacgccagc cccatgaagc
    12001 gctccatctc cacgctggcc ccgcgccccc acacggcccg gctcggcagc accgcgctgg
    12061 accgcccagc ccccagccag gccccgcacc atcaccacca ccgctgccac cgccgcaggg
    12121 acaggaagca gcggtccctg gagaagggtc ccagcctgtc tgctgacaca gatggggccc
    12181 ccgacagcac ggtggggcca ggactgccca cgggggaggg cccccccggc tgcaggcggg
    12241 agcgggagcg caggcaggag cggggccggt cccaggagcg gaggcagccc tcgtcctcct
    12301 cctcggagaa gcatcgcttc tactcctgtg accgcttcgg gggccgcgaa cccccacagc
    12361 ccaagccctc cctcagtagt caccccacgt cgccgacggc cgggcaggag ccgggacccc
    12421 acccgcaggg gagtggttcg gttcacggga gccccctgct gtccacatct ggtgctagca
    12481 ccccgggccg ggggcggagg cagctccccc agaccccctt gaccccccgc cccagcgtca
    12541 cctacaagac ggccaactcc tcgcctgtcc acttcgccgg ggcaccgagc ggcctccccg
    12601 ccttctcccc cggccggctc agccggggcc tctcagaaca caacgccctg ctgcagaggg
    12661 acccgctcag ccggcccctg gcgcccggct cgcgcatcgg ctccgacccc tacctggggc
    12721 agcggctgga cagcgaggcg cccgcccgtg ccctgcccga ggacgcgccc gcctttgagg
    12781 agaccgcggc cagcaactcg ggccgctcct cccggacctc gtacgtgtcc tcactgacct
    12841 cgcagccccc gccgctgcgc cgcgtgccca acggctacca ctgcactctg gggctgggcg
    12901 ggggcggccg ggcgcggcgc ggctgccacc accccgaccg cgaccgccgg tgctagacta
    12961 gttctagcaa gcttcgacga attctgcaga tatccatcac actggcggcc gctcgagcat
    13021 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
    13081 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    13141 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    13201 tctgagtagg tgtcattcta ttctgggggg tggggt
//
