LOCUS       sequence-410489-        4579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DV1 prM-E structural cassette in pVRC8400.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Chao LH, Jang J, Johnson A, Nguyen A, Gray NS, Yang PL, Harrison SC
  TITLE     How small-molecule inhibitors of dengue-virus infection interfere 
            with viral membrane fusion.
  JOURNAL   Elife. 2018 Jul 12;7. pii: 36461. doi: 10.7554/eLife.36461.
  PUBMED    29999491
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-07-12"; volume: "7. pii: 36461. doi"; pages: " 
            10.7554/eLife.36461"
COMMENT     Sequence Label: DV1 VLPs pVRC8400
FEATURES             Location/Qualifiers
     source          1..4579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2218..2240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2434..2453)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2553..2575
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2706..2725)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2717..3526
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2784..2803)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3704..4292
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4193..4212
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4446..4463
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacaccaag cttgcatgca ggcctctgca gtcgacgggc ccgggatccg atgcggccgc
      181 ttatgcctgc accatcacgc ccagatacag agtcaccatc cccactgcga tacaagtcat
      241 tgacagagag gtggagcggc tattcaggcc cagccaagtc agcaggatgc caattccgat
      301 cttcatggtc catgacactc cactaaacag gactccatag gctgtcccga agatctggtg
      361 aatcagtttc cccacgctgg taaagacccc gccgattgat ccgaaatccc atgcagtgtc
      421 ccccagaatt gccattcgtc gagctcctcg agcggttgcc tcaaacatct tgccgataga
      481 ggatcctttc ttgaaccagg acagtttcag ggccttttcc cctgcgccca cgacgatgta
      541 gctctcgcca aaagggggtt cggcctcaat gttcactggt ttctccttat ctgtgacgat
      601 aggatttgcg gtaatcagtc gcccgttctg ggtcacgccc ttctcatcct ggcttgagaa
      661 agggatttta cagggagcgt ctgtgccttc atacttcacc tggaccagca cggttccatg
      721 ctgagtctcg gccacttctt tctccagctt aaaactccca gtacacatca cgtaagacat
      781 gcccttcagt gtcagtttgt ccatcttcag cctgcatttc aggtggccgg cgaagatagt
      841 tgtggttcca ctagactgaa tctcagtagc gcctgtcagg gcggtatgca ttgctccttc
      901 ctggcttccc agcacgacca cctcctgttt ctttgcgtga gcagtcttga atgtcaccag
      961 cagatcctgg cgattccatg tctcctgtga ggtactggct ccgctggtcc atggcagtgg
     1021 caggtccaga aaccactgtt tatgcaccag ccagcttttc ttttccattg tcagcagcac
     1081 catctcgttg aaatccagtc cggtccttgg actgcaatcc agagtcagag ccccgtagtc
     1141 tgtcagctgg atctcagagg ttggagcctg aggtgtaatg gtggcagttg tgccgtgttc
     1201 ggtagtctca tttcccactt gatgctggtc cccggtgtgg acagtcacga tgacagaata
     1261 cttcaggttt tcgtactgca caattttccc ctccagctta gtgacacatt taaacttggc
     1321 gcatgtgatc agggatcctt tcccgaacag gccacatcca ttcccccagc ccctatccac
     1381 aaaggtgcgc ctgcagacga agttagtgtc ctgttcctcc accagagtag cttcgccctg
     1441 tgtaggacat ctggagtctg tggtagtatt gctgattttt gcctcaatgc acagcttccg
     1501 cagcacagca gggtttgtga cttcggtctt cagcagctcg atatccaggg tgggtttgtc
     1561 ctttgccatt gtggtcacac atgagccgtg ttccaggacc acatcgaccc atgtagctcc
     1621 actcagcccc tccacaaagt ccctgtttcc gatcccgacg catctcatgg ccatagaggg
     1681 ggtcaccagc atcagcagaa tgaaaatgat ccccttctga gtgatggatg tgccaatggc
     1741 atgtgccaga aacagagcga tcactgtgaa gcctgggtgt ctcagggccc aggtttccac
     1801 tttctgaatc tgcttccagg ctccctcgga gctcatccaa gtttctgttc gggtctccag
     1861 tcccagtccc acatgtggag ccagggcgac agatcgctta tctctccggt gttcgccggt
     1921 ctgggaacag gttccataag tcacccaagt ctctgtggca ttacaccagc agtcgacatc
     1981 gtcgggttcg gtctcagtga tccgtggaca cttgtatgtc atggtatctt cgcacagctc
     2041 ccccaggtcc atggcaatca gggtgcacat gttcacgcct gcgctggttt tgaacagcag
     2101 actttttcct cgctcctgtt ttgagacgat catgtgtggc tcgcctcccc tggttgtcag
     2161 atggaagcta gcatctagat gtattcgcga ggtaccgagc tcgaattctc tggccgtcgt
     2221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2341 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     2401 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     2461 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     2521 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2581 accgtcatca ccgaaacgcg cgatgcagct ctggcccgtg tctcaaaatc tctgatgtta
     2641 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2701 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2761 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2821 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2881 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2941 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3001 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3061 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3121 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3181 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3241 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3301 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3361 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3421 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3481 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3541 ttggttgtaa cattattcag attgggcttg atttaaaact tcatttttaa tttaaaagga
     3601 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3661 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3721 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3781 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3841 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3901 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3961 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4021 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4081 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4141 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4201 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4261 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4321 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4381 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4441 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4501 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4561 gcagtgagcg caacgcaat
//
