LOCUS       sequence-410750-        9763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0230
FEATURES             Location/Qualifiers
     source          1..9763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2701..3289
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2754..2774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3172..3183)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3361..3594
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3672..3793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3709..3728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3763..3782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3833..3968
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3988..4007)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3989..4007)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4017..4034)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4017..4033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4026..4048)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4175..4630
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4262..4281)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4472..4493
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4656..4760
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4761..4829,4830..5621)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4979..4998)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5792..6380
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6281..6300
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6534..6551
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6668..6689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6704..6709,6710..6727,6728..6734)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6742..6758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6747..6769
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6766..6782
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6766..6782
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6801..6821
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6803..6821
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6849..7075
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7076..7256
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7303..7428
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7921..8154
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8339..8383
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8614..8824
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8636..8832
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8683..8818
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8864..9466
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8864..8883)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9360..9380
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9509..9626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 acacctaact caacatacag gaattcatgc tggagagaaa ccctacaaat gtgaaaaatg
     1261 tggcaaagcc tttaataggt ccacatcact tagtaaacat aagagaattc atactggaga
     1321 gaaaccctac acatgtgaag aatgtggcaa agcctttaga cggtccacag ttctgaacga
     1381 acataagaaa attcatactg gagagaaacc ctacaaatgt gaagaatgtg gcaaagcctt
     1441 tacaaggtcc acaacactga atgaacacaa gaaaattcat actggagaga aaccctacaa
     1501 atgtaaagaa tgtggcaaag cctttagatg gtccacaagc ctgaatgaac ataagaatat
     1561 tcatactgga gagaaaccct acaaatgtaa agaatgtggc aaagccttta gacagtccag
     1621 gagcctgaat gaacataaaa atattcatac tggcgaaaaa ccctacacat gtgaaaaatg
     1681 tggcaaagct tttaaccaat cctcaagtct tattatacac aggagcattc attctgaaca
     1741 aaaactttac aaatgtgaag aatgtggcaa agcctttact tggtcctcat cccttaataa
     1801 acataagaga attcatactg gagagaaacc ctacacatgt gaagaatgtg gcaaagcttt
     1861 ttataggtcc tcacaccttg ctaaacataa gagaattcat actggagaga aaccctacac
     1921 gtgcgaagaa tgtggcaaag cttttaacca atcctcaact cttatattac acaagagaat
     1981 ccattctggg caaaaacctt acaaatgtga agaatgtggc aaagccttta cacggtccac
     2041 aacactgaac gaacataaga aaattcatac tggcgagaaa ccctacaaat gtgaagaatg
     2101 tggcaaagct ttcatatggt ccgcaagcct gaatgaacat aagaatattc atactggaga
     2161 gaaaccctac aaatgtaaag aatgtggcaa agcttttaac caatcctcag gccttattat
     2221 acacaggagc attcattctg aacaaaaact ttacaaatgt gaagaatgtg gcaaagcctt
     2281 tactcggtcc acagccctga atgaacataa gaaaattcat tctggagaga aaccctacaa
     2341 atgcaaagaa tgtggcaaag cctataactt atcctcaacc cttactaaac ataagagaat
     2401 tcatactgga gagaaaccct tcacatgtga agaatgtggc aaagccttca attggtcctc
     2461 atcccttact aaacataaga taattcatac tggagagaaa tcctacaaat gtgaagaatg
     2521 tggcaaagct tttaatcggc cctcaaccct tactgtacac aagcgaattc atactggcaa
     2581 ggaacatagt tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     2641 tatatcttgt ggaaaggacg aggcgatgat agtgcaggcg gtcgtacgcg ttaagtcgac
     2701 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2821 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2881 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2941 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3001 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3061 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3121 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3181 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3241 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     3301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3361 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     3601 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     3661 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3721 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3781 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     3841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3901 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3961 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4021 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4081 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4141 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4201 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4261 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4321 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4381 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4441 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4501 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4561 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4621 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4681 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4741 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4801 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4861 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4921 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4981 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5041 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5101 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5161 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5221 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5281 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5341 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5401 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5461 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5521 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5581 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5641 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5701 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5761 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5821 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5881 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5941 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6001 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6061 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6121 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6181 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6241 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6301 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6361 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6421 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6481 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6541 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6601 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6661 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6721 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6781 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     6841 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     6901 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     6961 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7021 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7081 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7141 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7201 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7261 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7321 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7381 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7441 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7501 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7561 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     7621 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     7681 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     7741 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     7801 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     7861 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     7921 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     7981 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8041 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8101 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8161 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8221 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8281 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8341 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8401 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8461 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8521 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     8581 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     8641 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8701 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8761 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8821 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     8881 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     8941 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     9001 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9061 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9121 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9181 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9241 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     9301 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     9361 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     9421 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
     9481 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9541 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     9601 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
     9661 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
     9721 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
