LOCUS       sequence-410751-       13986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KAN(1-131)-NpuDnaE(N)_GFPuv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13986)
  AUTHORS   Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan 
            GA, Yang X
  TITLE     Split selectable marker systems utilizing inteins facilitate gene 
            stacking in plants.
  JOURNAL   Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8.
  PUBMED    37237044
REFERENCE   2  (bases 1 to 13986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-05-26"; volume: "6(1):567. doi"; pages: " 
            10.1038/s42003-023-04950-8"
COMMENT     Sequence Label: pAXY0008
FEATURES             Location/Qualifiers
     source          1..13986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2400..2648
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3337..3353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3337..3353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3350..3372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3361..3377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3385..3391,3392..3409,3410..3415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3430..3451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3642..4318
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4425..4444)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    5077..5251
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5329..5353
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5778..6572
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6659..7247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7148..7167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7401..7418
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7433..7573
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7499..7518)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7659..7681
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7917..8111
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8177..9250)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9679..10308)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11609..11633
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11655..11907)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11939..11956)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11963..13780,13971..13985))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13781..13970
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagat
     2401 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     2461 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     2521 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2581 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2641 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2701 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2761 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2821 tttagtacta attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg
     2881 cgaacctgat tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt
     2941 tttggtagtc tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt
     3001 tgcaatttgt tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat
     3061 gaagttatat accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact
     3121 tatgattgtc ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc
     3181 ttttgtcgtt gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa
     3241 agaagagctc aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta
     3301 gatgccacct gacgtctaag aagaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3541 tttgcgtatt ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc
     3601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     3661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     3721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     3781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt
     3901 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3961 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4021 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4081 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4141 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4201 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     4261 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     4321 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatatg gggattgaac
     4381 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4441 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4501 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caggacgagg
     4561 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4621 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4681 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4741 atacgcttga tccggctacc tgcttgtcct acgagaccga gattcttact gttgagtatg
     4801 gattgcttcc aatcggcaag atcgtagaga aacgtattga gtgtacagtt tattctgttg
     4861 acaacaacgg aaatatctat actcagcctg ttgctcaatg gcacgataga ggggaacaag
     4921 aggttttcga gtattgcctt gaggatggta gcctcattcg agcaacaaaa gatcacaaat
     4981 ttatgacagt ggatggtcag atgttaccta tcgatgaaat tttcgaaagg gaattagacc
     5041 tcatgagagt ggataacctc cctaattagg ctcgagtttc tccataataa tgtgtgagta
     5101 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
     5161 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     5221 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta
     5281 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     5341 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     5401 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     5461 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     5521 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     5581 gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt
     5641 gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt
     5701 gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa
     5761 gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg
     5821 aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg
     5881 tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct
     5941 atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa
     6001 aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg
     6061 gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt
     6121 atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata
     6181 gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg
     6241 attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt
     6301 taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca
     6361 acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg
     6421 cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag
     6481 aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa
     6541 aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt
     6601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6781 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7261 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7321 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7381 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     7441 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     7501 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     7561 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7621 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7681 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     7741 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     7801 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     7861 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     7921 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     7981 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     8041 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     8101 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     8161 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     8221 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     8281 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     8341 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     8401 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     8461 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     8521 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     8581 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     8641 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     8701 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     8761 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     8821 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     8881 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     8941 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     9001 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
     9061 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     9121 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     9181 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
     9241 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc
     9301 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
     9361 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
     9421 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
     9481 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
     9541 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
     9601 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
     9661 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
     9721 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
     9781 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
     9841 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
     9901 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
     9961 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    10021 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    10081 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    10141 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    10201 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    10261 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    10321 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    10381 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    10441 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    10501 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
    10561 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
    10621 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    10681 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    10741 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    10801 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    10861 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    10921 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    10981 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    11041 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    11101 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    11161 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    11221 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    11281 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    11341 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    11401 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    11461 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    11521 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
    11581 tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg
    11641 atagtttaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt
    11701 tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat
    11761 aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta
    11821 attcaacaga aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa
    11881 actttattgc caaatgtttg aacgatcggg gaaattcgag ctggtcacca attcacacgt
    11941 gatggtgatg gtgatggcta gcgttcttgt agccgaaatc tggaatgttg gtccagcgct
    12001 cgcgaaagac gtgcgcggcg agcttcggct tgcggtcacg agtgaacacg cccttcttgt
    12061 ttccttggac gcgcatcacg ccctgagagg tcgcgaagtc cgcgaagttc cacgcttgct
    12121 cacccacgaa gttctcaaac tcatcgaaca cgacgtggtt cgcctggtag tactcgactt
    12181 gatattcctc ggtgaacatc actggatcaa tgtcgtgaaa gcccgcaacg gtgtctgcgc
    12241 cgtactcagt gatcatgatc ggctttcctg ggcaacgctt gttccacgcg tgaaattcct
    12301 ggcggagatg gactttggcc gcttcgagat caccgccatc gaagtaccat ccgttatagc
    12361 gattgagcgc gatgacgtca atcagttcgg cgactttgtc cgtctccggg gtagccatca
    12421 caaacagcac gatcgtgacc ggacgcttct gtgggtcgag ttccttggtc agctccacca
    12481 acggcttgaa gtactcgtac gcgccctctt cctcagtcgc cgcctcgttg gcgatgctcc
    12541 acatcacgac gcttggatgg ttcttgtcac gagacaccag ttcacggaga acgtcttgat
    12601 ggtgctcaaa cgtccgaatc ttctcccagg tactgacgcg ctcgctgcct tcgccgagtc
    12661 ccgtggtggc catgaagttg aggtgcacgc caactgccgg agtctcgtcg atcacgacca
    12721 gaccctcgcg atccgcaaga cgcatcaact cttcagagta cggatagtgt gcggtccgga
    12781 agctgttggc gccgatccat ttgaggatat tgaaatccat cacattgctc gcttcgttaa
    12841 agccacggcc gttgatagga gtgtcctcat gtttgccaaa gcccttgaag tagaacggtt
    12901 tgttgttgat gaggaacttg ccgtcgttga cttccacggt ccgcacgccg aacggctctt
    12961 catagacatc gatggtcagt ccgtcgttca ccagttccac tttgatctgg tagagatacg
    13021 tgttcagtgg ttcccagagg atgacattcg gaatctccac gttaccgctc aggccctcgg
    13081 tgcttgcgac cactttgcct tcctcatcca cgaccgacac tttcacggtc tcggctttgc
    13141 cttgaaagtc caccgtatag gtcacagtcc cggttgggcc attgaagtcg gtcacaaccg
    13201 agatgtcctc gacgtacgta aacggggtcg tgtagatttt caccggacgg tgcaggcctg
    13261 catagttgaa gaagtcgaag ttcggcttgt tacgaatgac ttttccgagg ccctcttcgt
    13321 ggcgctcgct gtacagcccc accgggaggg tgctatcgtc gaggatgttg tccacggcga
    13381 cggtgacgcg attcatgcca tcacgcagcg agttgttgat ttccgcttcg aatggcagga
    13441 atccgccctt gtgctccacg accagctcac cattgacata gacaattgct ttgtgagttg
    13501 cagagccgaa gcggagcacg atacgctgat ccttcagata ggccggcacc gtgaactcac
    13561 gttcgtacca gacatatccg atatggttgc ggatttcctt ggtcacgcca atgtcattgt
    13621 aactgcttgg gacggccata ctaatagtgt cggtcagctt gctttcgtac cacttctctt
    13681 ccagtccttt cccgtagtcc agcttgaagt tccagacgcc attgaggtcg aagacgccac
    13741 gggtctcggt gttgatcggg tacagactag ttcgtcggtt ctgtaactat catcatcatc
    13801 atagacacac gaaataaagt aatcagatta tcagttaaag ctatgtaata tttacaccat
    13861 aaccaatcaa ttaaaaaata gatcagttta aagaaagatc aaagctcaaa aaaataaaaa
    13921 gagaaaaggg tcctaaccaa gaaaatgaag gagaaaaact agaaatttac cctcagatct
    13981 accatg
//
