LOCUS       sequence-410775-       13749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HYG(1-52)-NpuDnaE(N)_GFPuv.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Yuan G, Lu H, De K, Hassan MM, Liu Y, Islam MT, Muchero W, Tuskan 
            GA, Yang X
  TITLE     Split selectable marker systems utilizing inteins facilitate gene 
            stacking in plants.
  JOURNAL   Commun Biol. 2023 May 26;6(1):567. doi: 10.1038/s42003-023-04950-8.
  PUBMED    37237044
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-05-26"; volume: "6(1):567. doi"; pages: " 
            10.1038/s42003-023-04950-8"
COMMENT     Sequence Label: pAXY0012
FEATURES             Location/Qualifiers
     source          1..13749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2400..2648
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3337..3353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3337..3353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3350..3372)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3361..3377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3385..3391,3392..3409,3410..3415))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3430..3451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3642..4318
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     polyA_signal    4840..5014
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5092..5116
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5541..6335
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6422..7010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6911..6930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7164..7181
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7196..7336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7262..7281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7422..7444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7680..7874
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7940..9013)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9442..10071)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11372..11396
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(11418..11670)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(11702..11719)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(11726..13543,13734..13748))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          13544..13733
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctatcttcat gctgcaacga acggaggtta
     1981 caccaacacc aggaaggaga tctatgaaga cggcggcatc ttggaggtca acttccgtta
     2041 cacttacgag ttcaacaaga tcatcggtga cgtcgagtgc attggacatg gattcccaag
     2101 tcagagtccg atcttcaagg acacgatcgt gaagagttgt cccacggtgg acctgatgtt
     2161 gccaatgtcc gggaacatca tcgccagctc ctacgcttac gccttccaac tgaaggacgg
     2221 ctctttctac acggcagaag tcaagaacaa catagacttc aagaatccaa tccacgagtc
     2281 cttctcgaag tcagggccca tgttcaccca cagacgtgtc gaggagactc tcaccaagga
     2341 gaaccttgcc atagtggagt accagcaggt tttcaacagc gccccaagag acatgtagat
     2401 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     2461 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     2521 aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2581 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2641 aagataagat gggctcatag agtaaaacga ggcgagggac ctataaacct cccttcatca
     2701 tgctatttca tgatctattt tataaaataa agatgtagaa aaaagtaagc gtaataaccg
     2761 caaaacaaat gatttaaaac atggcacata atgaggagat taagttcggt ttacgtttat
     2821 tttagtacta attgtaacgt gagactacgt atcgggaatc gcctaattaa agcattaatg
     2881 cgaacctgat tagattcacc gaccctccta tcgtgtcgac ctttctgttt cttagaattt
     2941 tttggtagtc tatgtactaa taatgtcagc ttcgtattta tttcataagc aatttgcatt
     3001 tgcaatttgt tttttacttt tatttttatt gtattgtgga atgtggactc gtaccaacat
     3061 gaagttatat accaccaaaa aaattacagt tagtcaaaag attcacgagt gagagctact
     3121 tatgattgtc ttttacgtat atgtctaatt gtctatttgc tcaataatct ttgtactttc
     3181 ttttgtcgtt gataaaatca caaagttcca aaagtaatcg aatgatttgc ttttaagaaa
     3241 agaagagctc aataattcaa catatatctg tacacaatta ccgcctttga gtgagctcta
     3301 gatgccacct gacgtctaag aagaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     3361 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3421 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3481 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3541 tttgcgtatt ggctagagca gcttgccaac atggtggagc acgacactct cgtctactcc
     3601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     3661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     3721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     3781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgaacatggt
     3901 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     3961 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4021 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4081 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4141 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4201 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     4261 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     4321 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatatg aaaaagcctg
     4381 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     4441 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     4501 gatatgtcct gcgggtaaat agctgcttgt cctacgagac cgagattctt actgttgagt
     4561 atggattgct tccaatcggc aagatcgtag agaaacgtat tgagtgtaca gtttattctg
     4621 ttgacaacaa cggaaatatc tatactcagc ctgttgctca atggcacgat agaggggaac
     4681 aagaggtttt cgagtattgc cttgaggatg gtagcctcat tcgagcaaca aaagatcaca
     4741 aatttatgac agtggatggt cagatgttac ctatcgatga aattttcgaa agggaattag
     4801 acctcatgag agtggataac ctccctaatt aggctcgagt ttctccataa taatgtgtga
     4861 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
     4921 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     4981 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
     5041 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     5101 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     5161 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     5221 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     5281 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     5341 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
     5401 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
     5461 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
     5521 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
     5581 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     5641 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     5701 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     5761 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     5821 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     5881 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     5941 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     6001 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     6061 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     6121 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     6181 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     6241 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     6301 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
     6361 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6421 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6481 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6541 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6601 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6661 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6721 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6781 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6841 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6901 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6961 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7081 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7141 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     7201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     7261 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7321 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7381 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     7441 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     7501 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     7561 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     7621 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     7681 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     7741 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     7801 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     7861 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     7921 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     7981 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     8041 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     8101 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     8161 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     8221 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     8281 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     8341 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     8401 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     8461 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     8521 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     8581 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     8641 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     8701 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     8761 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     8821 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     8881 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     8941 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     9001 tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca
     9061 ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac
     9121 ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca
     9181 tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca
     9241 gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg
     9301 ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg
     9361 cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca
     9421 ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg
     9481 gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc
     9541 agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc
     9601 ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac
     9661 aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag
     9721 gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc
     9781 tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa
     9841 gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac
     9901 ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga
     9961 atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat
    10021 ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc
    10081 ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg
    10141 ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag
    10201 ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc
    10261 acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg
    10321 atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt
    10381 cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac
    10441 ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc
    10501 ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt
    10561 cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc
    10621 caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta
    10681 gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg
    10741 tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc
    10801 gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg
    10861 tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc
    10921 tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc
    10981 gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg
    11041 cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc
    11101 gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa
    11161 cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga
    11221 ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg
    11281 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
    11341 gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca
    11401 ctgatagttt aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta
    11461 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
    11521 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
    11581 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
    11641 gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gagctggtca ccaattcaca
    11701 cgtgatggtg atggtgatgg ctagcgttct tgtagccgaa atctggaatg ttggtccagc
    11761 gctcgcgaaa gacgtgcgcg gcgagcttcg gcttgcggtc acgagtgaac acgcccttct
    11821 tgtttccttg gacgcgcatc acgccctgag aggtcgcgaa gtccgcgaag ttccacgctt
    11881 gctcacccac gaagttctca aactcatcga acacgacgtg gttcgcctgg tagtactcga
    11941 cttgatattc ctcggtgaac atcactggat caatgtcgtg aaagcccgca acggtgtctg
    12001 cgccgtactc agtgatcatg atcggctttc ctgggcaacg cttgttccac gcgtgaaatt
    12061 cctggcggag atggactttg gccgcttcga gatcaccgcc atcgaagtac catccgttat
    12121 agcgattgag cgcgatgacg tcaatcagtt cggcgacttt gtccgtctcc ggggtagcca
    12181 tcacaaacag cacgatcgtg accggacgct tctgtgggtc gagttccttg gtcagctcca
    12241 ccaacggctt gaagtactcg tacgcgccct cttcctcagt cgccgcctcg ttggcgatgc
    12301 tccacatcac gacgcttgga tggttcttgt cacgagacac cagttcacgg agaacgtctt
    12361 gatggtgctc aaacgtccga atcttctccc aggtactgac gcgctcgctg ccttcgccga
    12421 gtcccgtggt ggccatgaag ttgaggtgca cgccaactgc cggagtctcg tcgatcacga
    12481 ccagaccctc gcgatccgca agacgcatca actcttcaga gtacggatag tgtgcggtcc
    12541 ggaagctgtt ggcgccgatc catttgagga tattgaaatc catcacattg ctcgcttcgt
    12601 taaagccacg gccgttgata ggagtgtcct catgtttgcc aaagcccttg aagtagaacg
    12661 gtttgttgtt gatgaggaac ttgccgtcgt tgacttccac ggtccgcacg ccgaacggct
    12721 cttcatagac atcgatggtc agtccgtcgt tcaccagttc cactttgatc tggtagagat
    12781 acgtgttcag tggttcccag aggatgacat tcggaatctc cacgttaccg ctcaggccct
    12841 cggtgcttgc gaccactttg ccttcctcat ccacgaccga cactttcacg gtctcggctt
    12901 tgccttgaaa gtccaccgta taggtcacag tcccggttgg gccattgaag tcggtcacaa
    12961 ccgagatgtc ctcgacgtac gtaaacgggg tcgtgtagat tttcaccgga cggtgcaggc
    13021 ctgcatagtt gaagaagtcg aagttcggct tgttacgaat gacttttccg aggccctctt
    13081 cgtggcgctc gctgtacagc cccaccggga gggtgctatc gtcgaggatg ttgtccacgg
    13141 cgacggtgac gcgattcatg ccatcacgca gcgagttgtt gatttccgct tcgaatggca
    13201 ggaatccgcc cttgtgctcc acgaccagct caccattgac atagacaatt gctttgtgag
    13261 ttgcagagcc gaagcggagc acgatacgct gatccttcag ataggccggc accgtgaact
    13321 cacgttcgta ccagacatat ccgatatggt tgcggatttc cttggtcacg ccaatgtcat
    13381 tgtaactgct tgggacggcc atactaatag tgtcggtcag cttgctttcg taccacttct
    13441 cttccagtcc tttcccgtag tccagcttga agttccagac gccattgagg tcgaagacgc
    13501 cacgggtctc ggtgttgatc gggtacagac tagttcgtcg gttctgtaac tatcatcatc
    13561 atcatagaca cacgaaataa agtaatcaga ttatcagtta aagctatgta atatttacac
    13621 cataaccaat caattaaaaa atagatcagt ttaaagaaag atcaaagctc aaaaaaataa
    13681 aaagagaaaa gggtcctaac caagaaaatg aaggagaaaa actagaaatt taccctcaga
    13741 tctaccatg
//
