LOCUS       sequence-410838-        4457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Near-infrared fluorescent protein miRFP704nano in bacterial plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Oliinyk OS, Pletnev S, Baloban M, Verkhusha VV
  TITLE     Development of bright red-shifted miRFP704nano using structural 
            analysis of miRFPnano proteins.
  JOURNAL   Protein Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709.
  PUBMED    37347539
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2023 Jun 22:e4709. doi: 10.1002/pro.4709."
COMMENT     Sequence Label: pBAD/His-miRFP704nano
FEATURES             Location/Qualifiers
     source          1..4457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(27..44)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(198..786)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(278..297)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(957..1748,1749..1817))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1580..1599
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1818..1909)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(1928..1955)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2047..2133)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2286..2303
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(2816..2833)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(2876..3160)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2934..2953)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             3187..4065
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(4221..4243)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    join(4329..4457,1..12)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4384..4403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1561 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaaa gagtttgtag
     1921 aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta
     1981 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc
     2041 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     2101 ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat
     2161 ggggagaccc cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt
     2221 caggtgggac caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc
     2281 gttctgattt aatctgtatc aggctgaaaa tcttctctca tccaccaaaa cagccaagct
     2341 tcgaattcag ctagctctgc tggatggcga tgcccatcac cacggcttgc tgtttcagga
     2401 actcgatttc ccaggtctgc cagctccgag gggcagccag ttggtgggcc accagcaggc
     2461 cccacagctt cttgcccttg atgatgggga cagccagaat ggcccgcacc tgaaactggc
     2521 ccagcaggtc tctgtagctc tccagcagat tggcctggta gatgtcggcg atggcctggt
     2581 atctgccgtg caagtattcc tcacctctgg tttccatgaa gtagcagtcc cgcacctggg
     2641 tcttcaggat gctgttccat ctgtcgtcca cggcttccac gacgacgctg ccggagtaat
     2701 cttcctcgaa ctggaacacg cacactctgt ccacttgcag gaactggcgc acctcggtca
     2761 cgatggtgtt cagcatcttg tccaggtttg ccatggtacc agctgcagat ctgccatggt
     2821 gatggtgatg gtggctcccc atggttaatt cctcctgtta gcccaaaaaa cgggtatgga
     2881 gaaacagtag agagttgcga taaaaagcgt caggtaggat ccgctaatct tatggataaa
     2941 aatgctatgg catagcaaag tgtgacgccg tgcaaataat caatgtggac ttttctgccg
     3001 tgattataga cacttttgtt acgcgttttt gtcatggctt tggtcccgct ttgttacaga
     3061 atgcttttaa taagcggggt taccggtttg gttagcgaga agagccagta aaagacgcag
     3121 tgacggcaat gtctgatgca atatggacaa ttggtttctt ctctgaatgg cgggagtatg
     3181 aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taatgcccat
     3241 ctggtggcgg gtttaacgcc gattgaggcc aacggttatc tcgatttttt tatcgaccga
     3301 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
     3361 aatcagggac gagaatttgt ttgccgaccg ggtgatattt tgctgttccc gccaggagag
     3421 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
     3481 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggg
     3541 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
     3601 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
     3661 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
     3721 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
     3781 agcgtcgcac agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag
     3841 ttagggatta gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc gaagctgctt
     3901 ttgagcacca cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa
     3961 ctctatttct cgcgggtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc
     4021 ggttgtgaag aaaaagtgaa tgatgtagcc gtcaagttgt cataattggt aacgaatcag
     4081 acaattgacg gcttgacgga gtagcatagg gtttgcagaa tccctgcttc gtccatttga
     4141 caggcacatt atgcatgccg cttcgccttc gcgcgcgaat tgatctgctg cctcgcgcgt
     4201 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     4261 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4321 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     4381 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4441 gatgcgtaag gagaaaa
//
