LOCUS       sequence-410956-       10943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG-tagged ABE8e fused to Gag through a linker sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Haldrup J, Andersen S, Labial ARL, Wolff JH, Frandsen FP, Skov TW, 
            Rovsing AB, Nielsen I, Jakobsen TS, Askou AL, Thomsen MK, Corydon 
            TJ, Thomsen EA, Mikkelsen JG
  TITLE     Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of 
            CRISPR/Cas ribonucleoprotein complexes supporting base editing, 
            prime editing and in vivo gene modification.
  JOURNAL   Nucleic Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676.
  PUBMED    37678882
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Sep 8:gkad676. doi: 10.1093/nar/gkad676."
COMMENT     Sequence Label: pLVNP3.0-ABE8e
FEATURES             Location/Qualifiers
     source          1..10943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        161..540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        541..744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     695..715
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     741..765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          878..1353
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    1419..1475
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1476..1485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3315..3380
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3390..3416
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3426..3446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4041..4064,4065..4067,4068..8141)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4041..8141
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8184..8204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8285..8679
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(9018..9035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9189..9777)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9269..9288)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9948..10739,10740..10808))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10571..10590
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10809..10913)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatcccctg agggggcccc catgggctag aggatccggc ctcggcctct gcataaataa
       61 aaaaaattag tcagccatga gcttggccca ttgcatacgt tgtatccata tcataatatg
      121 tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      781 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccc ctcgaagctt
      841 acatgtggta ccgagctcgg atcctgagaa cttcagggtg agtctatggg acccttgatg
      901 ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga gaagtaacag
      961 ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa aatgctttct
     1021 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
     1081 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
     1141 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
     1201 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
     1261 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
     1321 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
     1381 ctggcccatc actttggcaa agcacgtgag atctgctcag atctcgagct caagcttcga
     1441 attctgcagt cgacggtacc gcgggcccgg gatccgccac catgggtgcc agggccagcg
     1501 tgctgagcgg cggcgagctg gacaggtggg agaagatcag gctgaggccc ggcggcaaga
     1561 agaagtataa gctgaagcac atcgtgtggg ccagcaggga gctggagagg ttcgccgtga
     1621 accccggcct gctggagacc agcgagggct gcaggcagat cctgggccag ctgcagccca
     1681 gcctgcagac cggcagcgag gagctgagga gcctgtacaa caccgtggcc accctgtact
     1741 gcgtgcacca gaggatcgag atcaaggaca ccaaggaggc cctggacaag atcgaggagg
     1801 agcagaacaa gtccaagaag aaggcccagc aggccgccgc cgacaccggc cacagcagcc
     1861 aggtgagcca gaactacccc atcgtgcaga acatccaggg ccagatggtg caccaggcca
     1921 tcagccccag gaccctgaac gcctgggtga aggtggtgga ggagaaggcc ttcagccccg
     1981 aggtgatccc catgttcagc gccctgagcg agggagccac cccccaggac ctgaacacca
     2041 tgctgaacac cgtgggcggc caccaggccg ccatgcagat gctgaaggag accatcaacg
     2101 aggaggccgc cgagtgggac agggtgcacc ccgtgcacgc cggccccatc gcccccggcc
     2161 agatgaggga gccccgcggc agcgacatcg ccggcaccac cagcaccctg caggagcaga
     2221 tcggctggat gaccaacaac ccccccatcc ccgtgggcga aatctacaag aggtggatca
     2281 tcctgggcct gaacaagatc gtgaggatgt acagccccac cagcatcctg gatatcaggc
     2341 agggccccaa agagcccttc agggactacg tggacaggtt ctacaagacc ctgcgcgccg
     2401 agcaggccag ccaggaggtg aagaactgga tgaccgagac cctgctggtg cagaacgcca
     2461 accccgactg caagaccatc ctgaaggccc tgggacccgc cgccaccctg gaggagatga
     2521 tgaccgcctg ccagggcgtg ggcggccccg gccacaaggc cagggtgctg gccgaggcca
     2581 tgagccaggt gaccaacacc gccaccatca tgatgcagag gggcaacttc aggaaccaga
     2641 ggaagatggt gaagtgcttc aactgcggca aggagggcca caccgccagg aactgccgct
     2701 cccccaggaa gaagggctgc tggaagtgcg gcaaggaggg ccaccagatg aaggactgca
     2761 ccgagaggca ggctaatttt ttagggaaga tctggccttc ctacaaggga aggccaggga
     2821 attttcttca gagcagacca gagccaacag ccccaccatt tcttcagagc agaccagagc
     2881 caacagcccc accagaagag agcttcaggt ctggggtaga gacaacaact ccccctcaga
     2941 agcaggagcc gatagacaag gaactgtatc ctttaacttc cctcagatca ctctttggca
     3001 acgacccctc gtcacaatta agatcggtgg ccagctcaag gaggccctgc tggacaccgg
     3061 cgccgacgac accgtgctgg aggagatgtc cctgcccggc aggtggaagc ccaagatcat
     3121 cggcggcatc ggcggcttca tcaaggtcag gcagtacgac cagatcctca tcgagatctg
     3181 cggccacaag gccatcggca ccgtgctggt gggacctaca cctgtcaaca tcatcggcag
     3241 gaacctgctc acccagatcg gctgcaccct caacttctaa ctttagccag aactatccga
     3301 ttgtgcagac cggagactat aaggaccacg acggagacta caaggatcat gatattgatt
     3361 acaaagacga tgacgataag ggcggctccc ctgctgctaa acgtgttaag cttgatgggg
     3421 gtagcccaaa gaagaagcgg aaagtctctg aggtggagtt ttcccacgag tactggatga
     3481 gacatgccct gaccctggcc aagagggcac gggatgagag ggaggtgcct gtgggagccg
     3541 tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc ggcctgcacg
     3601 acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc atgcagaact
     3661 acagactgat tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg
     3721 ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca aaaagaggcg
     3781 ccgcaggctc cctgatgaac gtgctgaact accccggcat gaatcaccgc gtcgaaatta
     3841 ccgagggaat cctggcagat gaatgtgccg ccctgctgtg cgatttctat cggatgccta
     3901 gacaggtgtt caatgctcag aagaaggccc agagctccat caactccgga ggatctagcg
     3961 gaggctcctc tggctctgag acacctggca caagcgagag cgcaacacct gaaagcagcg
     4021 ggggcagcag cggggggtca gacaagaagt acagcatcgg cctggccatc ggcaccaact
     4081 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     4141 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     4201 gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     4261 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     4321 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     4381 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     4441 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     4501 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     4561 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     4621 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     4681 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     4741 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     4801 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     4861 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     4921 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     4981 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     5041 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     5101 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     5161 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     5221 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     5281 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     5341 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     5401 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     5461 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     5521 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     5581 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     5641 gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     5701 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     5761 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     5821 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     5881 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     5941 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     6001 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     6061 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     6121 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     6181 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     6241 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     6301 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     6361 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     6421 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     6481 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     6541 actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca
     6601 aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     6661 tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga
     6721 gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     6781 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     6841 tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag
     6901 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     6961 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     7021 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     7081 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     7141 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     7201 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg
     7261 ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg
     7321 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     7381 acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     7441 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     7501 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     7561 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     7621 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     7681 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     7741 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     7801 aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg
     7861 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca
     7921 acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc
     7981 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     8041 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     8101 tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctctggcggc tcaaaaagaa
     8161 ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtctaaaca ccccaccagt
     8221 gcaggctgcc tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtatca
     8281 ctaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa
     8341 ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa
     8401 catttatttt cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga
     8461 atgtgggagg tcagtgcatt taaaacataa agaaatgaag agctagttca aaccttggga
     8521 aaatacacta tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc taatgcacat
     8581 tggcaacagc ccctgatgcc tatgccttat tcatccctca gaaaaggatt caagtagagg
     8641 cttgatttgg aggttaaagt tttgctatgc tgtattttac attacttatt gttttagctg
     8701 tcctcatgaa tgtcttttca ctacccattt gcttatcctg catctctcag ccttgactcc
     8761 actcagttct cttgcttaga gataccacct ttcccctgaa gtgttccttc catgttttac
     8821 ggcgagatgg tttctcctcg cctggccact cagccttagt tgtctctgtt gtcttataga
     8881 ggtctacttg aagaaggaaa aacagggggc atggtttgac tgtcctgtga gcccttcttc
     8941 cctgcctccc ccactcacag tgacccggaa tccctcgaca tggcagtcta gcactagtgc
     9001 ggccgcagat ctgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9061 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9121 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9181 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9241 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9301 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9361 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9421 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9481 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9541 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9601 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9661 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9721 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9781 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9841 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9901 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9961 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10021 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10081 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10141 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10201 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10261 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10321 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10381 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10441 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10501 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10561 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10621 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10681 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10741 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10801 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10861 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10921 ccccgaaaag tgccacctga cgt
//
