LOCUS       sequence-411028-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1695..1721
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1728..1754
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1761..1787
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1803..1805,1806..2519)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1803..1805,1806..2516)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2106..2125)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2453..2474
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2564..2581)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2570..2794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2840..3268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2927..2946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3137..3158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3277..3297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3282..3611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3462..3597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3524..3543
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3678..4472
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3732..3751)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4342..4361
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4646..4767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4683..4702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4737..4756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4816..4832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4816..4832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4829..4851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4840..4856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4864..4870,4871..4888,4889..4894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4909..4930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5047..5064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5218..5806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5298..5317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5977..6768,6769..6837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6600..6619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6838..6942)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7017..7036)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7208..7587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7588..7791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7742..7762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7836..7855
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7836..7854
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      7880..7889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggctgacct ggacctggcc cggggagact ttgcccaggc tgcccagcag ctgtggctgg
       61 ccctgcgggc actgggccgg cccctgccca cctcccacct ggacctggct tgtagcctcc
      121 tctggaacct catccgtcac ctgctgcagc gtctctgggt gggccgctgg ctggcaggcc
      181 gggcaggggg cctgcagcag gactgtgctc tgcgagtgga tgcaagcgcc agcgcccgag
      241 acgcagccct ggtctaccat aagctgcacc agctgcacac catggggaag cacacaggcg
      301 ggcacctcac tgccaccaac ctggcgctga gtgccctgaa cctggcagag tgtgcagggg
      361 atgccgtgtc tgtggcgacg ctggccgaga tctatgtggc ggctgcattg agagtgaaga
      421 ccagtctccc acgggccttg cattttctga cacgcttctt cctgagcagt gcccgccagg
      481 cctgcctggc acagagtggc tcagtgcctc ctgccatgca gtggctctgc caccccgtgg
      541 gccaccgttt cttcgtggat ggggactggt ccgtgctcag taccccatgg gagagcctgt
      601 acagcttggc cgggaaccca gtggaccccc tggcccaggt gactcagcta ttccgggaac
      661 atctcttaga gcgagcactg aactgtgtga cccagcccaa ccccagccct gggtcagctg
      721 atggggacaa ggaattttcg gatgccctcg ggtacctgca gctgctgaac agctgttctg
      781 atgctgcggg ggctcctgcc tacagcttct ccatcagttc cagcatggcc accaccaccg
      841 gcgtagaccc ggtggccaag tggtgggcct ctctgacagc tgtggtgatc cactggctgc
      901 ggcgggatga ggaggcggct gagcggctgt gcccgctggt ggagcacctg ccccgggtgc
      961 tgcaggagtc tgagagaccc ctgcccaggg cagctctgca ctccttcaag gctgcccggg
     1021 ccctgctggg ctgtgccaag gcagagtctg gtccagccag cctgaccatc tgtgagaagg
     1081 ccagtgggta cctgcaggac agcctggcta ccacaccagc cagcagctcc attgacaagg
     1141 ccgtgcagct gttcctgtgt gacctgcttc ttgtggtgcg caccagcctg tggcggcagc
     1201 agcagccccc ggccccggcc ccagcagccc agggcaccag cagcaggccc caggcttccg
     1261 cccttgagct gcgtggcttc caacgggacc tgagcagcct gaggcggctg gcacagagct
     1321 tccggcccgc catgcggagg gtgttcctac atgaggccac ggcccggctg atggcggggg
     1381 ccagccccac acggacacac cagctcctcg accgcagtct gaggcggcgg gcaggccccg
     1441 gtggcaaagg aggcgcggtg gcggagctgg agccgcggcc cacgcggcgg gagcacgcgg
     1501 aggccttgct gctggcctcc tgctacctgc cccccggctt cctgtcggcg cccgggcagc
     1561 gcgtgggcat gctggctgag gcggcgcgca cactagagaa acttggcgat cgccggctgc
     1621 tgcacgactg tcagcagatg ctcatgcgcc tgggcgctgg gaccactgtc acttccagcg
     1681 gagaattcgg atcataccca tacgatgttc cagattacgc tagtggctat ccttatgacg
     1741 tacctgatta tgcaggcagt tacccatacg atgtaccaga ctacgctggt accgtatcgg
     1801 gcgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1861 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1921 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1981 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2041 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2101 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2161 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2221 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2281 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2341 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2401 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2461 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     2521 cggccgctcg agtctagtgg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2581 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2641 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2701 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2761 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2821 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3181 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3241 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3361 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3421 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3481 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3541 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3601 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     3661 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3721 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3781 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3841 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3901 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3961 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4021 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4081 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4141 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4201 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4261 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4321 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4381 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4441 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4501 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4561 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4621 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4681 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4741 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4801 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4861 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4921 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4981 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6961 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     7021 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7081 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7141 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     7201 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7261 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     7321 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     7381 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     7441 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     7501 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     7561 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     7621 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     7681 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     7741 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     7801 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
     7861 caagctggct agcggatccg ccaccatggc ctctatgacc ggcggccagc aaatgggtat
     7921 ggcctctatg accggcggcc agcaaatggg agcatcgatg gacgagccac ccttcagcga
     7981 ggcggctttg gagcaggcgc tgggcgagcc gtgcgatctg gacgcggcgc tgctgaccga
     8041 catcgaaggt gaagtcggcg cggggagggg tagggccaac ggcctggacg ccccaagggc
     8101 gggcgcagat cgcggagcta tggattgcac tttcgaagac atgcttcagc ttatcaacaa
     8161 ccaagacagt gacttccctg gcctatttga cccaccctat gctgggagtg gggcaggggg
     8221 cacagaccct gccagccccg ataccagctc cccaggcagc ttgtctccac ctcctgccac
     8281 attgagctcc tctcttgaag ccttcctgag cgggccgcag gcagcgccct cacccctgtc
     8341 ccctccccag cctgcaccca ctccattgaa gatgtacccg tccatgcccg ctttctcccc
     8401 tgggcctggt atcaaggaag agtcagtgcc actgagcatc ctgcagaccc ccaccccaca
     8461 gcccctgcca ggggccctcc tgccacagag cttcccagcc ccagccccac cgcagttcag
     8521 ctccacccct gtgttaggct accccagccc tccgggaggc ttctctacag gaagccctcc
     8581 cgggaacacc cagcagccgc tgcctggcct gccactggct tccccgccag gggtcccgcc
     8641 cgtctccttg cacacccagg tccagagtgt ggtcccccag cagctactga cagtcacagc
     8701 tgcccccacg gcagcccctg taacgaccac tgtgacctcg cagatccagc aggtcccggt
     8761 cctgctgcag ccccacttca tcaaggcaga ctcgctgctt ctgacagcca tgaagacaga
     8821 cggagccact gtgaaggcgg caggtctcag tcccctggtc tctggcacca ctgtgcagac
     8881 agggcctttg ccgaccctgg tgagtgccgc aaccatcttg gcaacagtcc cactggtcgt
     8941 agatgcggag aagctgccta tcaaccggct cgcagctgcc agcaaggccc cggcctctgc
     9001 ccagagccgt ggagagaagc gcacagccca caacgccatt gagaagcgct accgctcctc
     9061 catcaatgac aaaatcattg agctcaagga tctggtggtg ggcactgagg caaagctgaa
     9121 taaatctgct gtcttgcgca aggccatcga ctacattcgc tttctgcaac acagcaacca
     9181 gaagcttaag caggagaacc taagtctgcg cactgctgtc cacaaaagca aatctctgaa
     9241 ggatctggtg tcggcctgtg gcagtggagg gaacacagac gtgctcatgg agggcgtgaa
     9301 gactgaggtg gaggacacac tgaccccacc cccctcggat gctggctcac ctttccagag
     9361 cagccccttg tcccttggca gcaggggcag tggcagcggt ggcagtggca gtgactcgga
     9421 gcctgacagc ccagtctttg aggacagcaa ggcaaagcca gagcagcggc cgtctctgca
     9481 cagccgcggc atgctggacc gctcccgcct ggccctgtgc acgctcgtct tcctctgcct
     9541 gtcctgcaac cccttggcct ccttgctggg ggcccggggg cttcccagcc cctcagatac
     9601 caccagcgtc taccatagcc ctgggcgcaa cgtgctgggc accgagtcta gagatggccc
     9661 tggctgggcc cagtggctgc tgcccccagt ggtctggctg ctcaatgggc tgttggtgct
     9721 cgtctccttg gtgcttctct ttgtctacgg tgagccagtc acacggcccc actcaggccc
     9781 cgccgtgtac ttctggaggc atcgcaagc
//
