LOCUS       sequence-411169-        6634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of the N-terminus, Domain I and the I-II loop (amino 
            acids 1-483) of rabbit Cav2.2 calcium channel.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin 
            AC
  TITLE     Dominant-negative synthesis suppression of voltage-gated calcium 
            channel Cav2.2 induced by truncated constructs.
  JOURNAL   J Neurosci. 2001 Nov 1;21(21):8495-504.
  PUBMED    11606638
REFERENCE   2  (bases 1 to 6634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2001-11-1"; volume: "21(21)"; pages: "8495-504"
COMMENT     Sequence Label: Cav2.2 DomI pMT2
FEATURES             Location/Qualifiers
     source          1..6634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..330)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15..150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     315..335
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          799..1028
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1011..1030
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2683..3246
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    3433..3567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3470..3489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3524..3543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4240..4257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4411..4999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4491..4510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5170..5961,5962..6030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5793..5812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6031..6135)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6203..6221
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6259..6281)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6381..6400
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6594..6616
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6608..6625
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6609..6625
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
       61 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
      121 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
      181 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
      241 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
      301 tggggacttt ccacacccta actgacacac attccacagg atccggtcgc gcgaatttcg
      361 agcggtgttc cgcggtcctc ctcgtataga aactcggacc actctgagac gaaggctcgc
      421 gtccaggcca gcacgaagga ggctaagtgg gaggggtagc ggtcgttgtc cactaggggg
      481 tccactcgct ccagggtgtg aagacacatg tcgccctctt cggcatcaag gaaggtgatt
      541 ggtttatagg tgtaggccac gtgaccgggt gttcctgaag gggggctata aaagggggtg
      601 ggggcgcgtt cgtcctcact ctcttccgca tcgctgtctg cgagggccag ctgttgggct
      661 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      721 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      781 aacctctcga ctgttggggt gagtactccc tctcaaaagc gggcatgact tctgcgctaa
      841 gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg gtgatgcctt
      901 tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca agcttgaggt
      961 gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact ttgcctttct
     1021 ctccacaggt gtccactccc aggtccaact gcaggcgagc ctgaattcct cgagggtacc
     1081 gggccccccc tcgaggtcga agcttgatca tggtccgctt cggggacgag ctgggcggcc
     1141 gctatggggg cgcgggcggc gcggagcggg cccggggcgg cggggccggc ggggctggcg
     1201 gcccgggccc cggggggctg ccgccgggcc agcgggtcct ctacaaacag tcgatcgcgc
     1261 agcgcgcgcg gaccatggcg ctgtacaacc ccatcccggt caagcagaac tgcttcaccg
     1321 tcaaccgctc gctcttcgtc ttcagcgagg acaacgtcgt ccgcaaatac gccaagcgca
     1381 tcaccgagtg gcctccgttc gagtacatga tcctggccac catcatcgcc aactgcatcg
     1441 tgctggccct ggagcagcac ctccctgacg gggacaagac gcctatgtcc gagcgcctgg
     1501 atgacaccga gccctacttc attgggatct tctgcttcga ggcggggatc aagatcctgg
     1561 ccctgggctt tgttctgcac aagggctcct atctgcggaa cggctggaac gtcatggact
     1621 tcgtggtagt cctcacgggg attcttgcca cagccggaac cgacttcgac ctgcgaacgc
     1681 tgagggctgt gcgtgtgcta aggcccctga agctggtgtc tgggattccg agcttgcagg
     1741 tggtgctcaa gtccatcatg aaggccatgg tcccgctgct gcagatcggg ctgctgctct
     1801 tcttcgccat cctcatgttt gccatcatcg gcctcgagtt ctacatgggc aagttccaca
     1861 aggcctgctt ccccaacagc acagacccag atcccgtggg tgacttcccc tgtggcaagg
     1921 aggccccggc ccggctctgt gagggagaca cggagtgccg ggagtactgg gccggaccca
     1981 actttggtat caccaacttt gacaacatcc tgtttgccat cttgaccgtg ttccagtgca
     2041 tcaccatgga gggctggact gacatcctct acaatacaaa cgatgcagcc ggcaacacgt
     2101 ggaactggct ctacttcatc cctctcatca tcatcggctc cttcttcatg ctcaacctgg
     2161 tgctgggtgt gctctcagga gagtttgcta aggagcgcga gagagtggag aaccgccgcg
     2221 ccttcctgaa gctccggcgg cagcagcaga ttgagcggga gctcaacggg tacctggagt
     2281 ggatcttcaa ggctgaggaa gtcatgctgg ctgaggagga caggaacgca gaggagaagt
     2341 cccctctgga tgcagtgttg aagagagcag ctgccaagaa gagcagaagc gacctgatcc
     2401 aggccgagga gggggagggc cggctcacag gcctctgtgc tcccggttcc ccgttcgccc
     2461 gtgccagcct gaaaagcggg aagacggaga gctcgtcgta cttccggagg aaggagaaga
     2521 tgttccgctt cttcatccgg cgcatggtga aggcacagta aactagtgcg gccgcaattc
     2581 cggggggggg gggggggggg acagctcagg gctgcgattt cgcgccaaac ttgacggcaa
     2641 tcctagcgtg aaggctggta ggattttatc cccgctgcca tcatggttcg accattgaac
     2701 tgcatcgtcg ccgtgtccca aaatatgggg attggcaaga acggagacct accctggcct
     2761 ccgctcagga acgagttcaa gtacttccaa agaatgacca caacctcttc agtggaaggt
     2821 aaacagaatc tggtgattat gggtaggaaa acctggttct ccattcctga gaagaatcga
     2881 cctttaaagg acagaattaa tatagttctc agtagagaac tcaaagaacc accacgagga
     2941 gctcattttc ttgccaaaag tttggatgat gccttaagac ttattgaaca accggaattg
     3001 gcaagtaaag tagacatggt ttggatagtc ggaggcagtt ctgtttacca ggaagccatg
     3061 aatcaaccag gccacctcag actctttgtg acaaggatca tgcaggaatt tgaaagtgac
     3121 acgtttttcc cagaaattga tttggggaaa tataaacttc tcccagaata cccaggcgtc
     3181 ctctctgagg tccaggagga aaaaggcatc aagtataagt ttgaagtcta cgagaagaaa
     3241 gactaacagg aagatgcttt caagttctct gctcccctcc taaagctatg catttttata
     3301 agaccatggg acttttgctg gctttagatc ataatcagcc ataccacatt tgtagaggtt
     3361 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3421 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3541 atcaatgtat cttatcatgt ctggatcccc ggccaacggt ctggtgaccc ggctgcgaga
     3601 gctcggtgta cctgagacgc gagtaagccc ttgagtcaaa gacgtagtcg ttgcaagtcc
     3661 gcaccaggta ctgatatccc accaaaaagt gcggcggcgg ctggcggtag aggggccagc
     3721 gtagggtggc cggggctccg ggggcgaggt cttccaacat aaggcgatga tatccgtaga
     3781 tgtacctgga catccaggtg atgccggcgg cggtggtgga ggcgcgcgga aagtcgcgga
     3841 cgcggttcca gatgttgcgc agcggcaaaa agtgctccat ggtcgggacg ctctggccgg
     3901 tgaggcgtgc gcagtcgttg acgctctaga ccgtgcaaaa ggagagcctg taagcgggca
     3961 ctcttccgtg gtctggtgga taaattcgca agggtatcat ggcggacgac cggggttcga
     4021 accccggatc cggccgtccg ccgtgatcca tgcggttacc gcccgcgtgt cgaacccagg
     4081 tgtgcgacgt cagacaacgg gggagcgctc cttttggctt ccttccaggc gcggcggctg
     4141 ctgcgctagc ttttttggcg agctcgaatt aattctgcat taatgaatcg gccaacgcgc
     4201 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5161 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5221 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     5281 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     5341 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     5401 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     5461 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     5521 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     5581 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5641 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5701 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5761 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5821 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5881 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5941 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6001 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6061 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6121 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     6181 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     6241 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     6301 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     6361 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6421 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
     6481 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6541 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6601 acgacgttgt aaaacgacgg ccagtgccaa gctt
//
