LOCUS       sequence-41137-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   O'Leary R, Reilly J, Hanson HH, Kang S, Lou N, Phillips GR
  TITLE     A variable cytoplasmic domain segment is necessary for 
            gamma-protocadherin trafficking and tubulation in the 
            endosome/lysosome pathway.
  JOURNAL   Mol Biol Cell. 2011 Sep 14.
  PUBMED    21917590
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2011 Sep 14."
COMMENT     Sequence Label: VCDstub-GFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(445..468)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(680..779)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(709..729)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tccctgaggc agcgtggggt cttctttcgt
       61 ttcaagtaaa tcttgaggta ttataagagg ctcgcttttc ccacagctct cctggctgat
      121 gagcgtgtct gcgtagttgg gttgtgggaa gatcaggtga ctcttccgag agcctgcggt
      181 aagtgaaact tcctgggagt aggtctgcag gaaagcttgc accccatcta gacccacaaa
      241 gtgagaggct ggtatgccag ccaatccatt tcctgaagcc tgtaggcgtg atgagtacca
      301 acgtcgaagc ctaagggcca gcagcacaat gacaaaggcc aggaagatgc aggagaccac
      361 agccactgcc accaccaggt agagtgtcag gtctgaattt tgggggttag caggggtatg
      421 gatgctttcc aggtcccggg atcctttgtc gtcgtcgtct ttgtagtctc cggtcagggc
      481 ctggcatccg ggtaggccat agacagcaga cagcaggaag aggaggaaga tggtggcagg
      541 cagagcttgg aggactcctg gacccccagg catcttctcc cagatcgatc cgagctcggt
      601 accgtcgact gcagaattcg aagcttgagc tcgagatctg agtccggtag cgctagcgga
      661 tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      721 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
      781 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      841 accgctatcc acgcccattg atgtactg
//
