LOCUS       sequence-411470-        5823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of full-length human WDR81. C-terminal NanoLuc 
            luciferase tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, 
            Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella
            PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A,
            Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR,
            Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, 
            Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, 
            Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, 
            Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, 
            Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, 
            Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, 
            Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards 
            AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, 
            Arrowsmith CH
  TITLE     A Target Class Ligandability Evaluation of WD40 Repeat-Containing 
            Proteins.
  JOURNAL   J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 
            10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4.
  PUBMED    39495097
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jmedchem.4c02010"; journalName: "J Med Chem"; date: 
            "2025-01-23- 23"; volume: "68"; issue: "2"; pages: "1092-1112"
COMMENT     Sequence Label: pNLF1-C_WDR81:1-1941
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcacagg gctctggggg tcgagaaggt gcactccgga cgcctgctgg tggatggcac
       61 agcccgcctt cccctgacat gcaggaattg cttcgctccg tagaaaggga tctctctata
      121 gatccacgcc agcttgcgcc ggcacctgga ggtacgcatg tcgtagccct ggtaccggcc
      181 aggtggctcg ccagtctgag agatagacga ctcccattgg gaccttgccc cagggctgag
      241 ggacttggtg aggcggaggt gcgaaccttg ctgcagaggt ctgtccagag gcttcctgcg
      301 ggttggacaa gggtagaggt gcacggtctc cgaaaacggc ggttgtccta tccgctgggt
      361 ggtggcctcc cttttgagga cggaagttgc ggccctgaaa ctctcacccg gtttatgcaa
      421 gaagtagccg cacaaaacta ccgaaatctg tggaggcatg cttatcatac gtacgggcaa
      481 ccgtattccc attcccctgc gccgagcgca gtacccgcac tggatagtgt gcgacaagct
      541 cttcagcggg tttatggttg ttcttttttg cccgttggcg aaacgacgca atgcccctct
      601 tacgctcgag agggaccttg tcctccacga gggagccctg cctgtccttc cttgttgcgg
      661 gcagaggctc tcctcgagag tccggagatg ctgtatgttg tgcaccctta cgtgcagttt
      721 agcctgcatg atgtggtgac cttcagccca gcaaagttga ctaatagtca ggcaaaagtg
      781 ctctttatct tgtttcgagt cctgcgagcc atggacgctt gccaccggca aggtctcgcc
      841 tgcggtgcat tgtctcttta ccatattgcc gtcgatgaga agctctgcag tgagcttaga
      901 ttggatctga gtgcttacga acggcctgag gaggacgaga atgaggaggc cccagtggcg
      961 cgagacgaag cgggtatcgt atcccaagag gagcagggtg gccaacccgg tcaacccacg
     1021 ggtcaagaag agcttagatc tctcgtgctt gactgggtcc acggcagaat ctccaacttc
     1081 cactatttga tgcaactcaa cagactggcg gggcggaggc agggtgaccc gaattaccat
     1141 ccagtcctgc cgtgggtggt agatttcaca accccacatg gtcggttccg agacctccgg
     1201 aaatctaagt tcaggttgaa caagggcgat aagcaactgg actttaccta tgaaatgact
     1261 cggcaggcct ttgtagcggg cggagcaggg gggggagagc ctccccacgt gccgcatcat
     1321 atctcagatg tcctgtccga catcacctac tatgtgtaca aagccagaag gactcctcgg
     1381 tctgttttgt gtggacacgt tcgcgcccaa tgggaacccc atgaatatcc agcgtcaatg
     1441 gagcgaatgc agaattggac gccagacgaa tgtattcctg agttctacac cgacccgagt
     1501 atatttcgat ctattcaccc cgatatgcct gacttggatg tgcccgcatg gtgctcaagt
     1561 agccaggagt tcgtagctgc ccatcgcgcc ttgttggaat cacgggaagt aagccgggat
     1621 ctgcaccatt ggattgacct tacattcgga tacaaattgc agggtaaaga ggcagttaag
     1681 gaaaagaacg tttgtcttca tctggttgac gcacacacgc atcttgcgtc ctacggggtt
     1741 gtccaattgt tcgatcaacc ccatccgcaa aggctcgctg gcgccccagc tcttgcccca
     1801 gaaccgccgc ttatacctaa acttctggtg cagacgatac aagaaaccac tggtcgagag
     1861 gattttaccg aaaatcctgg gcaattgccc aacggagtcg gcagaccagt attggaagca
     1921 accccctgtg aagcttcttg gactagggat agacctgtgg ccggtgaaga tgacctggag
     1981 caagccaccg aagcactgga tagtataagc ctcgcgggaa aggccgggga ccagctcgga
     2041 agcagctctc aagccagtcc tggcctcctc agcttttcag tcgcgagtgc ctcaagacct
     2101 gggcggcgca acaaagccgc cggtgccgac ccaggcgaag gggaggaagg gaggatattg
     2161 cttccagaag ggttcaatcc tatgcaggcc ttggaggagc tggaaaaaac aggaaacttc
     2221 cttgctaagg gccttggcgg cctgctggag gtcccagagc agcctagggt acagcccgcg
     2281 gtacccttgc agtgcctgtt gcaccgagac atgcaggcct tgggtgtcct cctcgcggaa
     2341 atggtgtttg cgacgcgagt ccgaacgctc caacctgacg ctcctttgtg ggtgcgcttt
     2401 caggcagtgc gcggactttg tacacggcac ccgaaggagg tgccagtttc ccttcagcct
     2461 gtattggata cattgttgca gatgtccgga ccggaggttc ccatgggtgc cgagcggggt
     2521 aagcttgacc aattgttcga gtatcgaccg gtgagtcaag gacttccacc cccatgccca
     2581 tcacagctgc tgtccccctt tagctcagta gttcccttcc ctccttattt cccggcactg
     2641 cataggttta ttctgcttta tcaggctcga cgcgtcgagg atgaggccca aggccgggag
     2701 ttggtattcg ccctctggca acagctgggc gctgtgctta aagacattac cccagaggga
     2761 cttgagatat tgctgccctt tgtcctcagc cttatgtccg aggaacatac cgcggtgtat
     2821 acagcctggt acctctttga gcctgttgcg aaggctctgg gacctaagaa cgcaaataag
     2881 tacctcctga aacccctcat aggagcttat gagtcccctt gccaattgca cgggaggttt
     2941 tatttgtata cagattgttt tgtggcccag ctcatggtaa gattgggcct gcaggccttt
     3001 ttgacgcacc tgttgccgca cgtactccaa gtattggccg gagctgaagc atcacaggaa
     3061 gagtcaaagg atctggcggg agctgctgaa gaagaagagt ccggtcttcc aggggcgggc
     3121 ccaggcagtt gcgcatttgg agaggaaatc ccgatggatg gcgaacctcc agcgtcttca
     3181 ggtctcggac tcccagacta tacaagcggg gttagcttcc acgatcaggc tgacctcccc
     3241 gagaccgaag atttccaagc tggtctgtat gtaaccgaaa gtccacaacc gcaagaagcg
     3301 gaggcagtgt ccttgggtcg actcagcgac aaatcttcta catccgaaac ttctctgggc
     3361 gaggagaggg caccggatga aggaggggcc cctgttgaca aaagctcact taggtccgga
     3421 gatagtagtc aagatctcaa acagtctgaa ggatccgaag aagaagagga ggaagaggat
     3481 tcctgtgttg tgctggagga ggaagaggga gaacaggagg aagttaccgg cgcaagtgaa
     3541 ctgacactta gtgatacggt gctctcaatg gagaccgtag tcgccggtgg ctcaggagga
     3601 gacggggaag aggaagagga ggccctgcca gaacaatcag aaggtaaaga acaaaagatc
     3661 ctccttgaca ccgcctgtaa gatggtccgc tggctctccg ctaagttggg accaacggtt
     3721 gcgagtcggc acgtggcgcg gaatctcctg agactgctca caagctgcta cgttggccct
     3781 actaggcaac agttcacagt ctcctctgga gaatcccccc cactctccgc tggaaacatc
     3841 taccagaaga gaccagtact gggtgacatc gtctctgggc cagtgttgag ctgcttgttg
     3901 cacatcgcac gattgtatgg cgaaccggta cttacgtatc aatacctgcc atatatttct
     3961 tacctcgtgg cccccggctc agcttctggg ccctcccgcc tcaatagccg aaaagaagcg
     4021 ggtctgcttg ctgctgtcac gttgactcaa aaaattattg tataccttag cgacacgaca
     4081 ctgatggaca tcttgccaag aatcagccat gaggtgctgc ttccagtctt gtcatttttg
     4141 accagtcttg ttacggggtt tccgtccggt gcccaagcaa ggactatcct ttgtgtaaaa
     4201 acaatatcat tgatagcact catttgcctt aggattggac aggagatggt acaacaacat
     4261 ttgtccgaac ccgtagctac ttttttccaa gtctttagtc agctgcacga gctccgccag
     4321 caggacttga agttggatcc tgctggtagg ggagagggcc aactgcccca ggtcgttttc
     4381 agcgatgggc aacaaaggcc agtcgacccg gccctcctgg atgaactcca aaaagttttc
     4441 accctggaaa tggcctacac gatatacgtg cctttttcat gccttttggg tgatataatc
     4501 agaaaaatta tccctaatca tgaactcgtt ggagaacttg ctgccctgta tcttgaaagc
     4561 atttcaccca gttcaaggaa tccagcaagt gttgaaccga caatgcctgg tacgggaccc
     4621 gagtgggatc ctcatggtgg tggctgcccg caagacgacg ggcattctgg tacattcggt
     4681 agcgtccttg tcggcaatcg cattcagata cctaatgact cccgccccga gaatcctggt
     4741 cctttgggac cgatatcagg ggtgggtggg ggaggactcg gaagcggctc cgacgacaat
     4801 gccctcaaac aggaactgcc acgaagtgtc cacggcctgt caggaaactg gctggcatac
     4861 tggcaatatg aaattggtgt tagtcagcaa gatgcccact tccatttcca tcaaatccga
     4921 cttcaatcct tccctggaca ctcaggtgca gtcaagtgtg tggcgcctct ttccagcgaa
     4981 gacttttttc tctcaggatc aaaagatcgg actgtacgct tgtggccact ctacaactat
     5041 ggtgatggca cttccgaaac agctcctagg ctggtttata ctcaacaccg aaaatcagta
     5101 tttttcgtag gacagctcga agcgccacag cacgttgtaa gctgtgacgg ggccgtgcac
     5161 gtatgggatc ctttcaccgg taagaccctg cgcactgttg agcctctcga ctctagggta
     5221 cccttgactg ccgtcgcagt tatgccggcc ccacatacgt ctatcaccat ggctagcagc
     5281 gactctacgt tgcggtttgt agactgtcgg aagcccggtc tgcagcacga attcagattg
     5341 ggaggtggac ttaaccccgg gctggtcaga gctttggcta tctctccatc aggccgctca
     5401 gtagtggccg gattttctag tgggttcatg gtcctgctcg acacgcggac cggtctggtt
     5461 cttcggggct ggcccgcgca tgaaggtgac attttgcaga tcaaagccgt tgaaggaagt
     5521 gtgctggtat catcatccag cgatcacagt ctcacggtgt ggaaagagct ggaacagaaa
     5581 ccgacacatc attacaaatc agcctcagat ccgatccata ccttcgattt gtacgggagt
     5641 gaagttgtga cgggcacggt ttccaacaag atcggcgttt gtagtttgct cgaaccaccc
     5701 tcacaggcca cgaccaagct cagtagtgag aattttcgag gaaccctcac aagtcttgcc
     5761 ctgctcccga caaaacgcca tctgctcttg ggatcagata atggcgttat taggttgctt
     5821 gca
//
