LOCUS       sequence-412467-        6461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_rDA3m in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell 
            MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang 
            H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring dopaminergic 
            activity in vivo.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w.
  PUBMED    38036855
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w."
COMMENT     Sequence Label: pAAV-hSyn-GRAB-rDA3m
FEATURES             Location/Qualifiers
     source          1..6461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2580..3168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2633..2653)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3051..3062)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3200..3676
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3399..3418)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3716..3856
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3716..3845
                     /label=AAV2 ITR
     rep_origin      3931..4386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4018..4037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4228..4249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4403..4422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4522..4544
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4582..4600)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4668..4772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4773..4841,4842..5633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4991..5010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5804..6392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6293..6312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   join(6454..6461,1..122)
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt cgggcgacct ttggtcgccc
       61 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
      121 ctgcggccgc acgcgtatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
      181 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
      241 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
      301 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
      361 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
      421 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
      481 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
      541 gtcgtgcctg agagcgcagt cgagaaaccg gtgccaccat gaggactctg aacacctctg
      601 ccatggacgg gactgggctg gtggtggaga gggacttctc tgttcgtatc ctcactgcct
      661 gtttcctgtc gctgctcatc ctgtccacgc tcctggggaa cacgctggtc tgtgctgccg
      721 ttatcaggtt ccgacacctg cggtccaagg tgaccaactt ctttgtcatc tccttggctg
      781 tgtcagatct cttggtggcc gtcctggtca tgccctggaa ggcagtggct gagattgctg
      841 gcttctggcc ctttgggtcc ttctgtaaca tctgggtggc ctttgacatc atgtgctcca
      901 ctgcatccat cctcaacctc tgtgtgatca gcgtggacag gtattgggct atctccagcc
      961 ctgagcggta tgagagaaag atgaccccca aggcagcctt catcctgatc agtgtggcat
     1021 ggaccttgtc tgtactcatc tccttcatcc cagtgcagct cagctggcac aaggcaaaac
     1081 ccacaagccc ctctgatgga aatgccactt ccctggctga gaccatagac aactgtgact
     1141 ccagcctcag caggacatat gccatctcat cctctgtaat aagcttttac atccctgtgg
     1201 ccatcatgat tgtctgctac accaggatct acaggattgc tcagaaaaag ctctcctcct
     1261 tcatggtttc cgagcggatg taccccgagg acggcgccct gaagagcgag atcaagaagg
     1321 ggctgaggct gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca
     1381 agaagcccgt gcagctgccc ggcgcctaca tcgtggacat caagttggac atcgtgtccc
     1441 acaacgagga ctacaccatc gtggaacagt gcgaacgcgc cgagggccgc cactccaccg
     1501 gcggcatgga cgagctgtac aagggaggta caggcgggag tctggtgagc aagggcgagg
     1561 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1621 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gaggcctttc
     1681 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     1741 cccctcagtt catgtacggc tccaaggcct acattaagca cccagccgac atccccgact
     1801 acttcaagtc gtccttcccc gagggcttca ggtgggagcg cgtgatgaac ttcgaggacg
     1861 gcggcattat tcacgtgaac caggactcct ccctgcagga cggcgtattc atctacaagg
     1921 tgaagctgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag aaaactatgg
     1981 ggtgggaaca tcggaaggac gaccttaaaa gagaaactaa agtcctgaag actctgtcgg
     2041 tgatcatggg tgtgtttgtg tgctgttggc tacctttctt catcttgaac tgcattttgc
     2101 ccttctgtgg gtctggggag acgcagccct tctgcattga ttccaacacc tttgacgtgt
     2161 ttgtgtggtt tgggtgggct aattcatcct tgaaccccat catttatgcc tttaatgctg
     2221 attttcggaa ggcattttca accctcttag gatgctacag actttgccct gcgacgaata
     2281 atgccataga gacggtgagt atcaataaca atggggccgc gatgttttcc agccatcatg
     2341 agccacgagg ctccatctcc aaggagtgca atctggttta cctgatccca catgctgtgg
     2401 gctcctctga ggacctgaaa aaggaggagg cagctggcat cgccagaccc ttggagaagc
     2461 tgtccccagc cctatcggtc atattggact atgacactga cgtctctctg gagaagatcc
     2521 aacccatcac acaaaacggt cagcacccaa cctgagaatt cgatatcaag cttatcgata
     2581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3001 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     3181 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     3241 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     3301 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3361 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3421 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3481 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3541 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3601 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3661 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     3721 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3781 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3841 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3901 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3961 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4021 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4081 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4141 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4201 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4261 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4321 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4381 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4441 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4501 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4561 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4621 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4681 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4741 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4801 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4861 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4921 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4981 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5041 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5101 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5161 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5221 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5281 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5341 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5401 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5461 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5521 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5581 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5941 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6421 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag g
//
