LOCUS       sequence-412503-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine STING gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmSTING
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(264..368)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(443..462)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        634..1013
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1015..1213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1168..1188
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1231..1411
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1458..1583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2076..2309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2494..2538
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2836..2953
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3004..3252
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3004..3024
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3175..3194
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3274..3349
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3411..3622
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3648..3668
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3669..4694
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             4695..4751
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4752..4754,4755..4757,4758..5468)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4752..4754,4755..4757,4758..5468)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5058..5077)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5405..5426
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5484..5999
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    6015..6603
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6068..6088)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6486..6497)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6675..6908
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6934..6951)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6940..7164
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7210..7638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7297..7316)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7507..7528
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7647..7667)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7652..7981
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7832..7967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7894..7913
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8029..8076
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8095..8469
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8599..8720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8636..8655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8690..8709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8769..8785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8769..8785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8782..8804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8793..8809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8817..8823,8824..8841,8842..8847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8862..8883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9000..9017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9171..9759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9251..9270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9930..10527,1..194,195..263))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
       61 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
      121 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
      181 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
      241 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
      301 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
      361 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
      421 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
      481 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
      541 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      601 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      661 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      721 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      781 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      841 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      901 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      961 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     1021 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     1081 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     1141 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     1201 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     1261 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1321 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1381 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     1441 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1501 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1561 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1621 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1681 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1741 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1801 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1861 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1921 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1981 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     2041 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     2101 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     2161 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     2221 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     2281 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     2341 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     2401 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     2461 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     2521 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2581 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2641 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2701 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2761 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     2821 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     2881 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     2941 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggt
     3001 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     3061 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     3121 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     3181 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3241 ggacgaaaca ccgcggcagt tatttcgaga ctcgttttag agctagaaat agcaagttaa
     3301 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt
     3361 cgctagctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     3421 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     3481 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     3541 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     3601 cgggtttgcc gccagaacac aggaccggtt ctagaggatc taccatgccc aagaagaaga
     3661 ggaaggtgtc caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
     3721 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
     3781 agcatacctg gaaaatgctt cagtccgttt gccggtcgtg ggcggcatgg tgcaagttga
     3841 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
     3901 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
     3961 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
     4021 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
     4081 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
     4141 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
     4201 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
     4261 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
     4321 aactggtcga gcgatggatt tccgtatctg gtgtagctga tgatccgaat aactacctgt
     4381 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
     4441 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
     4501 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
     4561 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     4621 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
     4681 tggaagatgg cgatgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga
     4741 atcctggacc gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     4801 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     4861 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     4921 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     4981 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     5041 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     5101 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     5161 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     5221 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     5281 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     5341 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     5401 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     5461 tgtacaagga tatctccgga ggtatggtct tcacactcga agatttcgtt ggggactggc
     5521 gacagacagc cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt
     5581 ttcagaatct cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg
     5641 ggctgaagat cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg
     5701 gccagatcga aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga
     5761 tcctgcacta tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg
     5821 gacggccgta tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc
     5881 tgtggaacgg caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt
     5941 tccgagtaac catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaaa
     6001 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6061 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6121 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6181 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6241 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6301 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6361 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6421 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6481 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6541 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6601 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6661 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6721 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6901 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6961 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     7021 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     7081 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     7141 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     7201 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7261 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7321 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7381 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7441 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7501 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7561 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7621 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7681 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7741 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7801 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7861 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7921 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7981 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     8041 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     8101 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     8161 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     8221 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     8281 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     8341 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     8401 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     8461 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8521 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8581 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8701 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     8761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    10501 tgagaatagt gtatgcggcg accgagt
//
